[摘要] 目的 对比2种不同表面材质培养瓶培养的人脐带间充质干细胞（human umbilical cord mesenchymal stem cells, hUC-MSCs）的培养特性。 方法 采集10条新鲜脐带，每条脐带用组织块法分离脐带组织并贴壁培养12d，收集原代细胞，分别取2*106个细胞用225cm2高粘培养瓶（简称A组）和225cm2普通瓶子（简称B组）细胞培养72h至P1代,收集P1代细胞继续培养72h至P2代，实验重复五次，观察比较两组实验组的P1和P2代培养瓶的细胞镜下形态、P2代比较两组实验组细胞消化时长、细胞扩增曲线、检测表面标志物、三系分化潜能，分别收集细胞培养至P4代比较SA?β?半乳糖苷酶染色阳性率。结果 2组细胞形态均为扁平长梭形。 P2代A组细胞消化时间为（123.8 ±3.09）s，B组细胞消化时间（38.5 ±2.20）s,差异有统计学意义(P<0.001)。A组的P0到P2代细胞扩增倍数为（129.49±0.89）倍，显著高于B组（101.4±1.67）倍，差异有统计学意义(P<0.001)。A组SA?β?半乳糖苷酶染色阳性率（2.58±0.44）%显著低于B组（4.79±0.33）%，且均符合间充质干细胞的质量标准。结论 相同接种密度的条件下，高粘培养瓶比普通培养瓶扩增倍数更高，且细胞衰老水平更低，优化了培养体系和培养效率，提高了细胞质量。
[Abstract] Objective: To compare the culture characteristics of human umbilical cord mesenchymal stem cells (hUC-MSCs) cultured in two different surface-material flasks. Methods: Ten fresh umbilical cords were collected, and umbilical cord tissue was isolated using the tissue block method and cultured adherently for 12 days. Primary cells were collected, and 2×10^6 cells were respectively seeded into 225 cm2 high-adhesion flasks (Group A) and 225 cm2 ordinary flasks (Group B) for culture for 72 hours until passage 1 (P1). P1 cells were collected and further cultured for 72 hours until passage 2 (P2). The experiment was repeated five times. The morphology of cells at P1 and P2 in both groups was observed under a microscope, and P2 cells were compared for digestion duration, cell growth curves, surface marker expression, and trilineage differentiation potential. Cells were also cultured to P4 to compare the SA?β-galactosidase positivity rate. Results: Cells in both groups displayed a flat spindle-shaped morphology. The digestion time of P2 cells in Group A was (123.8 ± 3.09) seconds, while in Group B it was (38.5 ± 2.20) seconds, showing a statistically significant difference (P<0.001). The cumulative cell amplification from P0 to P2 in Group A was (129.49 ± 0.89)-fold, significantly higher than that in Group B [(101.4 ± 1.67)-fold, P<0.001]. The SA?β-galactosidase positivity rate in Group A was (2.58 ± 0.44)%, significantly lower than in Group B(4.79 ± 0.33)%, and both met the quality standards for mesenchymal stem cells. Conclusion: Under the same seeding density, high-adhesion flasks yield higher cell amplification, lower cell senescence, and optimize the culture system and efficiency, thereby improving cell quality.
目的 分析2016—2024年宜春市5岁以下儿童死亡水平、死因构成、县区分布特征及变化趋势，为制定儿童健康干预策略提供依据。方法 回顾性收集2016—2024年宜春市5岁以下儿童死亡监测资料，统计各年度新生儿、婴儿及5岁以下儿童死亡率，分析死因构成比、顺位变化及县区分布特征，采用线性趋势χ2检验分析死亡率年度变化趋势。结果 2016—2024年宜春市各年龄段儿童死亡率均呈下降趋势（均P＜0.05），新生儿、婴儿、5岁以下儿童9年平均死亡率分别为1.35‰、2.62‰、5.02‰，较2016年分别下降26.79%、43.55%、43.06%。死因顺位整体呈现小幅变迁，肺炎占比逐步下降，溺水、意外窒息等意外伤害相关死因顺位持续前移。各县区死亡率存在差异，高死亡率县区集中在袁州区、奉新县，低死亡率县区以铜鼓县为主。结论 2016—2024年宜春市5岁以下儿童死亡率呈下降趋势，但意外伤害已成为主要死因，县区差异客观存在。应重点加强意外伤害预防工作，针对高死亡率县区实施差异化干预。
摘要：目的 分析2022—2024年深圳市福田区危重症孕产妇不同亚组（不同并发症/合并症）的流行病学特征、病因构成及相关因素，为优化区域孕产妇健康管理提供依据。方法 基于福田区危重症孕产妇监测网络，对2022年1月1日至2024年12月31日期间107921例孕产妇个案资料进行回顾性描述性分析，采用描述性流行病学方法及卡方检验分析不同因素与危重症主要并发症的关联。结果 深圳市福田区3年间危重症孕产妇共729例，总体发生率为6.75‰。各年度发生率分别为2022年6.67‰、2023年7.38‰和2024年6.33‰。危重症孕产妇中，≥35岁者占33.7%，本科及以上学历者占53.8%。初检妊娠风险评级为黄色者占比最高（41.98%），高危者占21.9%。最常见并发症为产后出血（43.89%），其次为宫缩乏力（17.28%）、前置胎盘（16.59%）、子痫/子痫前期（14.67%）和胎盘植入（13.99%）。血液系统疾病为最常见合并症（57.75%），内分泌系统疾病次之（24.42%）。单因素分析显示，产后出血的发生与高龄、产检次数不足5次、经产妇身份存在统计学关联（均P<0.05）；前置胎盘的发生与高龄、低学历、非汉族、初检高危评级、产检不足5次、经产存在统计学关联（均P<0.05）。结论 深圳市福田区危重症孕产妇发生率约为6.75‰，产后出血和前置胎盘为主要并发症。单因素分析显示，高龄、产检不足、初检高危分级及经产与上述主要并发症的发生相关。应加强动态妊娠风险管理和多学科协作，完善产科早期预警体系，以降低危重症孕产妇发生率，提高母婴安全水平。
Abstract: Objective To analyze the epidemiological characteristics, etiology composition, and related factors of critically ill pregnant women in Futian District, Shenzhen, from 2022 to 2024. Methods Based on the surveillance network, a retrospective descriptive analysis was conducted on 107,921 pregnant women. Chi-square tests were used to analyze the association between different factors and major complications. Results A total of 729 critically ill pregnant women were identified (overall incidence 6.75‰).??The most common complication was postpartum hemorrhage (43.89%), followed by uterine atony (17.28%), placenta previa (16.59%), eclampsia/preeclampsia (14.67%), and placenta accreta (13.99%). Univariate analysis showed that postpartum hemorrhage was statistically associated with advanced age, <5 antenatal visits, and multiparity (all P<0.05). Placenta previa was statistically associated with advanced age, low education level, non-Han ethnicity, high-risk initial assessment, <5 antenatal visits, and multiparity (all P<0.05). Conclusion The incidence of critically ill pregnant women in Futian District is approximately 6.75‰. Univariate analysis suggested that advanced age, insufficient antenatal visits, high-risk classification, and multiparity were associated with the main complications. Dynamic risk management and multidisciplinary collaboration should be strengthened.
目的: 探讨RNF213基因敲除对hCMEC/D3细胞分泌的外泌体circRNAs表达谱的影响。方法: 采用尺寸排阻法分别从RNF213-/- hCMEC/D3细胞（KO组）和野生型细胞（WT组）培养上清中提取外泌体，用Illumina Novaseq 6000 平台检测外泌体circRNAs表达谱，并用生物信息学方法进行分析。结果: 两组细胞存在134种差异表达的外泌体circRNAs，其中116种上调，18种下调。GO分析结果中，cellular nitrogen compound metabolic process (BP), intracellular anatomical structure (CC), heterocyclic compound binding (MF)和organic cyclic compound binding (MF)是最丰富的术语。Polycomb repressive complex通路是KEGG分析最显著富集的通路。circHIPK3可能通过ceRNA机制和/或与RNA结合蛋白相互作用参与烟雾病的发生发展，并成功构建circRNA-miRNA-mRNA通路。结论: 本研究确定了RNF213-/- hCMEC/D3细胞与野生型细胞外泌体circRNAs的差异表达谱，从外泌体的视角，为烟雾病的发病分子机制提供了新的见解。
