目的 探讨胃癌组织中刺猬信号通路(Hedgehog signaling pathway, Hh)中的音猬因子(Sonic hedgehog, Shh)和胶质瘤相关癌基因同源物-1(Glioma-associated oncogene homolog -1, Gli-1)与金属基质蛋白酶-2(Matrix metalloproteinase-2, MMP-2)的表达和临床意义。方法 采用免疫组织化学方法检测40例人胃癌组织、人胃息肉组织和40例正常胃黏膜组织中音猬因子、胶质瘤相关癌基因同源物-1、金属基质蛋白酶-2蛋白的表达。结果 胃癌组织中音猬因子、胶质瘤相关癌基因同源物-1、金属基质蛋白酶-2的阳性表达率分别为62.5%、67.5%、72.5%,高于胃息肉组织(阳性表达率分别为27.5%、37.5%、32.5%)和正常胃黏膜组织(阳性表达率分别为22.5%、17.5%、12.5%)(P<0.05);以上三者的表达与患者性别、年龄、组织学类型无关(P>0.05);而与分化程度、浸润深度、淋巴结转移相关(P<0.05);音猬因子、胶质瘤相关癌基因同源物-1、金属基质蛋白酶-2表达呈正相关。结论 刺猬信号通路可能通过某些机制可上调金属基质蛋白酶-2的表达,从而增强胃癌的侵袭性。联合检测胃癌组织中音猬因子、胶质瘤相关癌基因同源物-1、金属基质蛋白酶-2的表达水平在一定程度上可以作为胃癌预后的客观参考指标。

Objective To investigate the expression and clinical significance of Sonic hedgehog(Shh), Glioma-associated oncogene homolog -1(Gli-1) and Matrix metalloproteinase-2(MMP-2) in gastric cancer.Shh and Gli-1 are the molecules of Hedgehog(Hh) signaling pathway. MMP-2 is the member of matrix metalloproteinase family. Methods The expression of Shh,Gli-1 and MMP-2 proteins was examined by immunohistochemistry in the human gastric cancer tissues and the human gastric polyp and the normal gastric mucosa tissues of 40 cases. Results The positive expression rates of Shh,Gli-1 and MMP-2 in gastric cancer were 62.5%,67.5% and 72.5% respectively, which were significantly higher than those in the gastric polyp tissues (the positive expression rates were 27.5%,37.5% and 32.5% respectively) and normal gastric mucosa tissues (the positive expression rates were 22.5%,17.5% and 12.5% respectively),P<0.05.The expression of Shh,Gli-1 and MMP-2 was not correlated with the sex,age or histological type(P>0.05),but was correlated with depth of invasion,differentiation level and lymphonode metastasis in gastric cancer(P<0.05). The expression of Shh and Gli-1 was positive correlated with MMP-2. Conclusion Hedgehog(Hh)signaling pathway may have great effects on enhancing the invasive ability of gastric cancer by upregulating MMP-2 protein through some unknown mechanisms.The combined detection of the expression level of Shh,Gli-1 and MMP-2 in gastric cancer tissues might be used as an Objective references for assessing the prognosis of gastric cancer.

目的 探讨脉管炎围手术期出现焦虑症状及影响因素和护理以提高在围手术期的生活质量。方法 通过对脉管炎患者进行问卷调查焦虑发生及影响因素。结果 脉管炎围手术期患者出现焦虑是十分常见的,生活质量下降直接或间接影响手术的成功率。结论 有效的护理干预能改善各方面的焦虑因素,使患者在围手术期保持心情舒畅,从而适应截肢形象的改变,提高生活质量。

目的 探索在神经外科护理教学中使用周期目标教学模式对学生理论知识、护理技能的作用与意义。方法 采用统一标准百分制操作与理论考核,比较周期目标教学模式和一对一带教模式下护理学生护理理论与技能掌握、应用的差异。结果 实验组学生理论考核和操作考核成绩比一对一带教学生成绩高。结论 周期目标教学模式使学生的实习思路清晰,提高了学生实习的积极主动性;实习目标明确,促进了护生在专科技能操作上的提高。对提高我科护理教学质量起到积极的促进作用。

目的 探索外科硕士研究生医患沟通培养教育方法。方法 通过对56位外科硕士研究生的医患沟通培养实践,研究医患沟通教育的培养模式和评估方法。结果 通过课程培养,56位外科硕士研究生有92.8%的外科硕士研究生认为课程“有很大帮助”;培养前后对“信息采集”、“尊重隐私”、“语言艺术”、“情感沟通”、“诊疗作用”等方面认知有差异(P＜0.05),而在“专业知识”方面培养前后无差异(P＞0.05)。结论 应当在外科硕士研究生中建立医患沟通培养课程和相应的评估体系。

Objective To explore graduates training and education methods of communicating between doctors and patients in surgery department. Methods 56 graduates of surgery were trained for communicating between doctors and patients as special education practice.Experiences of the training were summed up to identify educational methods for graduates. Results After the end of the training programs,52 graduates,92.8% of 56,considered the training “great help”. Around the training of “Information Gathering”,“Respect for Privacy”,“Language Arts”,“Emotional Communication”,“Treatment Effects ” cognition of the graduates were significantly different from the beginning(P＜0.05),while in the “Professional Knowledge” no significant difference(P＞0.05). Conclusion Patient communication training courses and the corresponding evaluation system should be established.

目的 “看病难”这个与社会民生密切相关的难题,长期困扰广大民众。主要表现在看病流程复杂,挂号、候诊排队时间长,就诊指引不清晰,服务水平低。移动互联网时代,各个传统行业都在转变运营模式,医院应如何因势利导,寻求“破冰”良策。本文结合佛山市中医院的应用案例,对利用微信公众号^[1]改造现有业务流程,有效解决“看病难”问题进行讨论分析。

The problem “proper health care is difficult to get” is closely related to the people's livelihood,which has plagued the people for a long period of time.The difficulties mainly focused on the complex process,long-time waiting,unclear instruction and the low level of service.In the time of mobile internet,each traditional industry is shifting the operation mode.What the hospital should do to is to make the best use of the advantages to solve the problems.The purpose of the research is to optimize the current processes and to solve the problem of “Proper health care is difficult to get” by using Wechat public number combined with the cases in Foshan hospital of traditional Chinese medicine.

目的 调查分析病案首页疾病诊断存在的问题,以提出针对性的干预对策。方法 由病案科编码员根据《卫生部关于修订下发住院病案首页的通知》相关规定,对抽取的4200份病案首页的疾病诊断进行检查,对疾病诊断问题分类记录,同时对其发生原因进行问卷调查,建立相应数据库,应用Excel统计工具进行统计分析。结果 共748份的疾病诊断存在问题,发生率高达17.8%,包括疾病诊断中主要诊断选择错误8.3%、疾病诊断的名称不规范7.6%,以及漏诊问题1.8%。疾病诊断存在问题发生率较高的临床科室为内分泌科38.0%,骨科科32.8%,肿瘤科31.6%。在读研究生最容易出现疾病诊断问题,发生率高达48.0%。结论 对疾病诊断存在问题的高发临床医师群体和临床科室进行针对性干预对策,有望降低病案首页疾病诊断问题的发生率。

Objective To investigate the defects analysis and targeted intervention on the diseases diagnosis in the home pages of medical records. Methods To conduct an inspection analysis on the four thousand and two hundred medical records according to the related requirements of “Notification on Standard writing in Home Pages of Hospitalization Medical Records announced by the Ministry of Health” by professional medical records staff.They found out and classified the defects on the diseases diagnosis in the home pages of medical records,at the same time,questionnaire survey were carried on to investigate the related causes,and the corresponding database establish ed.Excel statistical tools were applied for statistical analysis. Results There were defects on the diseases diagnosis in a total of seven hundred and forty-eight cases,the incidence of which was 17.8%,including 8.3% of mistakes of main diagnosis,7.6% of lack of standardization in diagnosis names,1.8% of missed diagnosis respectively.Defects on the diseases diagnosis occurred in a higher rate in the clinical departments,such as department of endocrine with 38.0%,department of orthopedics with 32.8%,and department of oncology with 31.6%.Graduate students most prone to make mistakes with defects on the diseases diagnosis,the incidence of which was 48.0%. Conclusion Targeted interventions to clinician groups and clinical departments with high incidence of defects on the diseases diagnosis,is expected to reduce the incidence of which in the home pages of medical records.

目的 研究低视力儿童“学校-社区-家庭”三位一体综合康复干预工作的具体效果。方法 2013年1月,选择广州市盲人学校新入学80名低视力儿童,随机分为两组,为期二年的跟踪研究。其中A组执行“学校-社区-家庭”三位一体综合康复干预,B组执行传统康复干预。就知识积累、心理健康、生活能力三个方面对两组儿童的具体数据收集,研究“学校-社区-家庭”三位一体综合康复干预与传统干预方式之间的区别。结果 A组低视力儿童较B组儿童的文化知识积累与生活能力训练成绩均有提高,两者差距在正常分布带内,A组低视力儿童的心理素质优于B组低视力儿童。A组低视力儿童的综合评价效果高于B组儿童。结论 低视力儿童“学校-社区-家庭”三位一体综合康复干预工作的具体效果优于传统康复干预方式。

目的 观察采用桂枝茯苓汤联合甲氨蝶呤治疗异位妊娠的效果。方法 纳入90例异位妊娠患者,随机分为3组各30例,A组服用甲氨蝶呤,B组服用桂枝茯苓汤,C组采用桂枝茯苓汤联合甲氨蝶呤治疗,观察3组的治疗效果及附件包块变化情况。结果 3组治愈率整体比较,差异有统计学意义(P<0.05)。C组的治愈率与A组、B组分别比较,差异均有统计学意义(P<0.05)。3组附件包块治疗有效率整体比较,差异有统计学意义(P<0.05)。C组的有效率与A组、B组分别比较,差异均有统计学意义(P<0.05)。结论 桂枝茯苓汤联合甲氨蝶呤治疗异位妊娠,在降低β-HCG、症状改善、缩小包块方面均有较好效果,且副反应少、经济、方便。

目的 探讨青少年过敏性鼻炎与血清维生素D水平的相关性。方法 收集2012年4月—2014年4月经本院耳鼻喉科确诊的青少年过敏性鼻炎(AR)患者368例,为实验组;另选择同期本院健康体检者370例,为对照组。分别使用串联质谱仪检测其血清总维生素D、维生素D₂及维生素D₃浓度。结果 实验组中血清总维生素D、维生素D₂及维生的素D₃的检测结果分别为:20.92±3.35 ng/ml、3.64±0.82 ng/ml、18.83±3.62 ng/ml;对照组中血清总维生素D、维生素D₂及维生的素D₃的检测结果分别为:31.45±2.86 ng/ml、3.85±0.58 ng/ml、28.97±3.18 ng/ml;检测结果显示实验组与对照组总维生素D及维生素D₃水平均存在差异(P＜0.05),具有统计学意义,维生素D₂水平无差异(P＞0.05)。结论 青少年过敏性鼻炎血清维生素D水平比正常人群偏低,且以维生素D₃水平偏低为主,为临床诊治过敏性鼻炎患者提供重要依据。