目的 通过比较炎症性肠病(IBD)患者的血脂水平,探讨炎症性肠病疾病活动程度与血脂的相关性。方法 收集2013年1月—2018年5月在南方医科大学附属南海医院住院的159例IBD患者和159例健康对照为研究对象,检测分析两组的血浆TG、TC、LDL-C、HDL-C、脂蛋白a、白蛋白和超敏C反应蛋白(hCRP)水平差异,分析IBD患者疾病活动程度与血脂异常的关系。结果 与对照组比较,IBD患者的TG、TC、LDL-C、HDL-C和白蛋白均下降,但脂蛋白a升高(P＜0.05),且CD组的TC、LDL-C、HDL-C、白蛋白均较UC组更低(P＜0.05)。TC、LDL-C、HDL-C等胆固醇水平随IBD疾病活动程度加重而逐渐下降,且与hCPR呈负相关,脂蛋白a与hCRP呈正相关性,但未见TG水平与疾病活动相关。结论 IBD患者的胆固醇水平下降,脂蛋白a升高,CD患者更明显,胆固醇水平随IBD病情加重逐渐下降,且与hCRP呈负相关。

Objective To explore the correlations between disease activity of inflammatory bowel disease(IBD) and lipid profiles levels by compare the levels of plasma lipids in patients with IBD. Methods A total of 159 IBD patients admitted to Nanhai Hospital of Southern Medical University from January 2013 to May 2018 were included in the study and the clinical data were collected. There were 159 healthy people recruited in the control group. The differences of plasma levels of triglycerides(TG), total cholesterol(TC), low-density lipoprotein cholesterol(LDL-C), high-density lipoprotein cholesterol(HDL-C), lipoprotein(a), albumin and high-sensitivity C-reactive protein(hCRP) between these two groups were analyzed respectively. The relationships between lipids levels and the severity of IBD were analyzed. Results Plasma levels of TG,TC,LDL-C,HDL-C and albumin were lower in IBD group than those in control group,but lipoprotein(a) was higher than control group(P＜0.05). The levels of TC,LDL-C,HDL-C and albumin were lower in CD patients compared to those of UC(P＜0.05). Plasma levels of TC,LDL-C,HDL-C gradually decreased with the severity of IBD. TC,LDL-C,HDL-C values were negatively correlated with hCRP levels in IBD patients. And lipoprotein(a) values was positively correlated with hCRP levels in IBD patient. However, there was no association between TG levels and the severity of IBD. Conclusion TG、TC、LDL-C、HDL-C levels are decreased and lipoprotein(a) is increased in IBD patients, especially CD patients, compared with healthy controls. Moreover, the cholesterol levels are negatively associated with more severe disease activity.

目的 了解广州市海珠区肺结核患者登记情况及流行特征,为进一步做好海珠区肺结核防治提供依据。方法 收集2008—2017年海珠区结核病信息管理系统中按现住址登记的肺结核患者资料,采用描述性流行病学方法和相关统计方法对肺结核患者登记资料进行分析。结果 2008—2017年海珠区共报告登记肺结核患者 14 384 例,年报告发病率由2008年的105.3/10万下降到2017年的73.1/10万,呈下降趋势(χ²=164.973,P<0.001);男女发病相对比为2:1;各个年龄组均有发病,其中25～34岁年龄组发病人数最多,占总发病数的21.61%;职业分布方面,家务及待业人群最多,占总发病率34.48%,其次分别是其他(不固定职业),占17.29%,退休人群,占15.12%,学校学生占比5.52%。地区分布方面,各街道年平均发病率相差较大,最高为131.23/10万,最低的为66.40/10万,差异有统计学意义(χ² =164.973,P<0.001)。结论 广州市海珠区肺结核疫情呈逐年下降趋势,但部分街道肺结核发病率仍达100/10万以上。男性人群、25~34岁年龄组和低收入人群(家务及待业、不固定职业、退休人群)是防控的主要人群,学生肺结核发病人数多,要加强学校结核病防控和筛查工作。

Objective To understand the registration situation and epidemic characteristics of tuberculosis patients in Haizhu district of guangzhou, and to provide a basis for further prevention and treatment of tuberculosis in Haizhu district. Methods The data of tuberculosis patients registered at present address in the TB information management system of Haizhu district from 2008 to 2017 were collected, and the registration data of tuberculosis patients were analyzed using descriptive epidemiological methods and relevant statistical methods. Results From 2008 to 2017, 14 384 cases of tuberculosis patients were reported in Haizhu district. The annual reported incidence rate decreased from 105.3/100,000 in 2008 to 73.1/100,000 in 2017, showing a down ward trend (χ² = 164.973, P<0.001). The relative incidence of male and female was 2:1; all age groups had morbidity, of which 25~34 years old group had the largest number of cases, accounting for 21.61% of the total number of cases; in occupational distribution, household chores and unemployed were the most, accounting for 34.48 %, followed by other (non-fixed occupations), accounting for 17.29%, retired people, accounting for 15.12%, and school students accounting for 5.52%. In terms of regional distribution, the average annual incidence rate of each street varied greatly, with the highest being 131.23/100,000 and the lowest being 66.40/100,000. The difference is statistically significant(χ²=164.973,P<0.001). Conclusion The incidence of tuberculosis in Haizhu district of Guangzhou has been decreasing year by year, but the incidence of tuberculosis in some streets is still more than 100/100,000. Male population, 25~34 years old group and low-income population (domestic and underemployed, irregular occupation, retired population) are the main population for prevention and control. Students have a large number of cases of tuberculosis, so we need to strengthen prevention, control and screening of tuberculosis in schools.

目的 本研究旨在探讨2型糖尿病不同程度肾病与chemerin、SOD及MDA的相关性。方法 选取2016年1月—2017年12月期间于广州市第一人民医院内分泌科门诊和住院的患者100例,根据尿白蛋白/肌酐比(ACR)分为正常尿蛋白组(NA组,n=33),微量白蛋白尿组(MA组,n=34)及大量蛋白尿组(CA组,n=33),另选取32例我院体检中心体检结果正常的正常健康人作为对照组(NC组,n=32),测定血糖、糖化血红蛋白、血肌酐、ACR、24小时尿蛋白定量、胆固醇、甘油三酯、SOD、MDA、chemerin等水平。结果 SOD的水平:NC组> NA组> MA组>CA组(P<0.05);MDA的水平:CA组>MA组>NA组>NC组(P<0.05);Chemerin水平:CA组>MA组>NA组>NC组(P<0.05)。相关性分析提示ACR与血清SOD呈负相关,与MDA、chemerin呈正相关。多元回归分析显示,病程、胆固醇、糖化血红蛋白、chemerin是影响ACR的主要因素。结论 Chemerin、MDA、SOD可能参与糖尿病肾病的发生发展,检测其水平可以在一定程度上反映2型糖尿病肾病患者的病情严重程度。

Objective To explore the relationship between different type of Type 2 diabetes nephropathy and chemerin,SOD,MDA. Methods A total of 100 inpatients and outpatients were enrolled in this study between January 2016 and December 2017 in Guangzhou First People's Hospital. They were divided into normal urinary protein group (NA group, n=33), microalbuminuria group (MA group, n=34) and massive proteinuria group (CA group, n=33) based on ACR. Another 32 healthy people were collected as a control group in medical examination center (NC group, n=32). The levels of blood sugar, glycated hemoglobin, serum creatinine, ACR, 24-hour urinary protein, cholesterol, triglyceride, SOD, MDA and chemerin were measured. Results The level of SOD: NC group > NA group > MA group > CA group (P< 0.05). The level of MDA: CA group > MA group > NA group > NC group (P< 0.05). The level of chemerin: CA group > MA group > NA group > NC group (P< 0.05). Correlation analysis showed that ACR was negatively correlated with serum SOD and positively correlated with MDA and chemerin. Multivariate logistic regression demonstrated that course of disease, CHOL, HbA1c and chemerin were the main factors affecting ACR. Conclusion Chemerin, MDA and SOD may be involved in the occurrence and development of diabetic nephropathy. Chemerin, MDA and SOD may reflect the severity of type 2 diabetic nephropathy

目的 采用生物信息学方法预测低氧预处理人胎盘绒毛膜间充质干细胞环状RNAs相对应的miRNA及其靶基因,并分析靶基因所参与的生物学过程和信号通路。方法 用Arraystar公司的商业软件为环状RNAs预测其相对应的miRNAs,分别用targetScan7.1和mirdbV5数据库预测miRNAs的靶基因,并取两个预测结果的合集,应用在线网站http://www.geneontology.org和http://www.genome.ad.jp/kegg对靶基因进行功能富集分析和信号通路富集分析。结果 功能富集分析表明,circRNAs的靶基因主要涉及到细胞发育、细胞分化和细胞发育调控。东京基因和基因组百科全书信号通路富集分析表明肿瘤中转录失控和有丝分裂原激活蛋白激酶(MAPK)信号通路最有意义,而且分析发现MAPK信号通路为核心通路。本研究表明,低氧预处理使得间充质干细胞中部分circRNAs的表达量发生差异性变化。结论 低氧预处理人胎盘绒毛膜间充质干细胞环状RNAs同低氧预处理间充质干细胞的生物学特性变化密切有关,为了解低氧预处理影响间充质干细胞特性发生变化的分子机制提供新思路。

Objective To predict the miRNA and its target genes of circular RNAs in hypoxia- preconditioned human palcenta chorionic mesenchymal stem cells using bioinformatics, and analyze the biological process and signaling pathway. Methods Arraystar's commercial software was used to predict the corresponding miRNAs of circular RNAs. The target genes of miRNAs were predicted by targetScan7.1 and mirdbV5 databases respectively, and an intersection of two prediction results was obtained. The online databases http://www. geneontology.org and http://www.genome.ad.jp/kegg performed functional enrichment analysis and signal pathway enrichment analysis of target genes. Results Functional enrichment analysis indicated that the target genes of circRNAs mainly involved cell development, cell differentiation and cell development regulation. The signal enrichment analysis of the Tokyo Gene and Genome Encyclopedia indicates that transcriptional misregulation in cancer and mitogen-activated protein kinase (MAPK) signaling pathway are most meaningful, and the MAPK signaling pathway is found to be the core pathway. This study showed that hypoxic preconditioning caused significant changes in the expression of mesenchymal stem cell circRNAs. Conclusion The changes of circular RNAs in hypoxia-preconditioned human placental chorionic mesenchymal stem cell is closely related to the biological characteristics of hypoxia-preconditioned mesenchymal stem cells. This study provides a new idea for understanding the molecular mechanism of hypoxic preconditioning affecting the changes of biological characteristics in mesenchymal stem cells.

目的 探讨利用微信群保留机采血小板无偿献血者的应用效果。方法 从本血站穿越安全输血标准化管理系统中调取2015年1月1日—2018年12月31日捐献机采血小板无偿献血者信息,根据初次捐献机采血小板后是否自愿加入机采微信群的机采血小板无偿献血者分为实验组和对照组,统计分析各组机采血小板无偿献血者重复献血 2～3 次和≥4 次以上机采血小板无偿献血者人数及平均献血次数。结果 2015—2018年间机采血小板采集量、献血人数及平均献血次数逐年上升;重复献血 2～3 次和≥4 次以上人数,实验组均高于对照组,机采血小板平均献血次数实验组均高于对照组,其中2016年和2018年实验组机采血小板平均献血次数高于对照组(P<0.05)。结论 通过机采微信群预约与管理机采血小板无偿献血者可提高机采血小板招募的工作效率,有效扩大和稳定机采血小板无偿献血者固定队伍。

目的 探讨市区院前急救流行病学特征,为制定院前急救策略和合理调配急救资源提供参考依据。方法 采用回顾性资料收集方法选取我院2013—2014年院前急救患者14000例(对照组),2017年院前急救患者9 630例(观察组)作为研究对象,对两组的性别、年龄、死亡原因等建立数据库,采用描述性研究和圆形分布方法进行统计分析,并对比两组数据,分析院前急救患者变化趋势。结果 对照组急救患者性别男性所占比例高于女性,年龄45~55岁所占比例最高,死亡原因创伤所占比例最高,心脑血管疾病其次;观察组急救患者性别男性所占比例高于女性,但低于对照组男性所占比例,年龄45~55岁所占比例最高,但比例低于对照组,死亡原因创伤所占比例最高,心脑血管疾病其次。结论 根据院前急救时间分布规律合理调配急救资源,充分发挥院前急救功能,提高院前急救水平。

目的 探讨职业性噪声暴露对广州市黄埔区某企业噪声作业工人听力损失状况的影响。方法 观察2016年8月—2018年8月,广州市黄埔区某企业噪声作业工人年度职业健康体检1 600例为调查对象,对该群体的纯音听阈测试检查结果进行分析。结果 随工龄不同听力损失发生率相比,差异有统计学意义(P<0.05),还会随着工龄的增长而增加听力损失几率;佩戴听力防护用品工人听力损失发生率低于不佩戴听力防护用品的工人,差异有统计学意义(P<0.05)。结论 噪声作业工人工龄时间越长听力损失发生率越高,防护听力用品的使用可有效降低听力损伤发生率,故在噪声作业时,企业应做好听力损伤防护措施,保障工人身心健康。

目的 探讨影响初次机采血小板献血者再次捐献的相关因素,为制定机采献血者保留策略提供依据。方法 选择2016年1月1日—2016年12月31日在广州血液中心首次成功捐献机采血小板的13 899例献血者为研究对象,分析每个献血者献血时性别、年龄、文化程度、是否发生献血不良反应与下一年度是否再次捐献机采血小板的相关性。结果 男性献血者再次捐献率(10.68%)高于女性献血者再次捐献率(8.94%),差异有统计学意义(P＜0.01);献血者年龄18～25岁组再次捐献率最高为12.86%,46～60岁组再次捐献率最低为6.46%,差异有统计学意义(P＜0.001);献血者文化程度高中或职中组再次捐献率最高为11.23%,本科或以上组再次捐献率最低为8.94%,差异有统计学意义(P＜0.001);未发生不良反应献血者再次捐献率(10.37%)高于发生不良反应献血者再次捐献率(5.56%),差异有统计学意义(P＜0.01)。结论 男性、年龄较小、文化程度较低和未发生献血不良反应的献血者更愿意返回再次捐献机采血小板,对这部分献血者采取针对性保留措施,有利于建立起一支固定捐献机采血小板的献血者队伍。

Objective To explore the related factors affecting the re-donation of the first apheresis platelet donors, and to provide a basis for the retention strategy. Methods A total of 13 899 blood donors who successfully donated apheresis platelets for the first time in Guangzhou Blood Center from January 1, 2016 to December 31, 2016 were selected as subjects. The correlation among gender, age, education level, adverse reactions of blood donation and whether platelet collection was re-donated in the next year was analyzed. Results Re-donation rate of male blood donors (10.68%) was higher than that of female blood donors (8.94%). The difference was statistically significant (P<0.01). Re-donation rate of blood donors aged 18～25 was the highest 12.86%, and that of 46～60 was the lowest 6.46%. The difference was significant (P<0.001). Re-donation rate of high school or vocational college blood donors was the highest 11.23%. The lowest re-donation rate was 8.94% in the undergraduate group or above, and the difference was statistically significant (P< 0.001). The re-donation rate of donors without adverse reactions (10.37%) was higher than that of donors with adverse reactions (5.56%). The difference was statistically significant (P<0.01). Conclusion Male, younger, less educated and no adverse reactions to blood donation are more willing to return to donate platelet again. Targeting these donors for reserve measure will conducive to the establishment of a fixed platelet donor team.

目的 分析抽检血液成分关键指标结果并采用趋势分析方法进行评估。方法 收集整理2015—2017年每月1次的8种血液成分质量控制的检测数据,选择关键指标并制作折线图,联合行动限和警戒限进行趋势分析。结果 未能100%达到GB 18469—2012《全血及成分血质量要求》的项目包括悬浮红细胞容量及Hct、单采血小板及浓缩血小板血小板含量、新鲜冰冻血浆及冷沉淀VIII因子含量。新鲜冰冻血浆及冷沉淀的VIII因子含量项目分别出现10次和5次偏离数据;单采血小板及浓缩血小板的血小板含量项目分别出现3次和4次偏离数据。结论 根据血液成分的关键指标结果进行趋势分析发现问题,主动寻找原因并及时采取相应措施,是确保血液质量的一种有效手段。

Objective To analyze and evaluate the results of key indexes of the blood component by trend analysis. Methods Collected and arranged the data of eight kinds of blood component quality control,which was did once month in 2015-2017.Selected the key indexes and made the line charts.Allied with the action limits and warning limits for trend analysis. Results The key indexes could not 100% meet the “Quality requirements for whole blood and component blood”,including capacity and Hct of suspended red blood cell,platelet content of apheresis platelets and platelets concentrate,factor VIII content of fresh frozen plasma and cryoprecipitate.The factor VIII of fresh frozen plasma and cryoprecipitate showed ten and five deviation data, respectively, the platelet content of apheresis platelet and platelets concentrate showed three and four deviations, respectively. Conclusion It's an effective way to ensure blood quality by trend analysis based on the results of key indexes of blood components, and actively find the cause and take corresponding measures in time.