低氧预处理人胎盘绒毛膜间充质干细胞环状RNAs的生物信息学分析

来源期刊：广州医药 | 1-6 发布时间：2021-12-17 收稿时间：2025/11/13 17:31:55 阅读量：22

作者：: 郝文革,

孙逊沙,

吴洁莹,

陈劲松,

喻秋霞,

李焱,

吴韶清,

关键词：: 环状circRNA 低氧间充质干细胞生物信息学; Circular RNAs Hypoxia Mesenchymal stem cells Bioinformatics

DOI：: 10.3969/j.issn.1000-8535.2019.02.001

收稿时间：: 2018-11-10
修订日期：
接收日期：
引用总数：: 0

目的采用生物信息学方法预测低氧预处理人胎盘绒毛膜间充质干细胞环状RNAs相对应的miRNA及其靶基因,并分析靶基因所参与的生物学过程和信号通路。方法用Arraystar公司的商业软件为环状RNAs预测其相对应的miRNAs,分别用targetScan7.1和mirdbV5数据库预测miRNAs的靶基因,并取两个预测结果的合集,应用在线网站http://www.geneontology.org和http://www.genome.ad.jp/kegg对靶基因进行功能富集分析和信号通路富集分析。结果功能富集分析表明,circRNAs的靶基因主要涉及到细胞发育、细胞分化和细胞发育调控。东京基因和基因组百科全书信号通路富集分析表明肿瘤中转录失控和有丝分裂原激活蛋白激酶(MAPK)信号通路最有意义,而且分析发现MAPK信号通路为核心通路。本研究表明,低氧预处理使得间充质干细胞中部分circRNAs的表达量发生差异性变化。结论低氧预处理人胎盘绒毛膜间充质干细胞环状RNAs同低氧预处理间充质干细胞的生物学特性变化密切有关,为了解低氧预处理影响间充质干细胞特性发生变化的分子机制提供新思路。

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