目的 分析立体定向体部放射治疗(SBRJ)应用于孤立性肺肿瘤的疗效,探讨其临床价值。方法 采用拓能(TOPSLANE)全身X线立体定向放射治疗系统治疗15例孤立性肺肿瘤患者,其中I期非小细胞肺癌患者8例,单个肺转移瘤患者7例。单次剂量为5~8Gy／次,每天1次,每周3次,共8~10次,总剂量50~64Gy,生物有效剂量(BED)75~115Gy。分析治疗的近期疗效、急性放射损伤和局部控制率。结果 3例患者完全缓解(20.0%),10例患者部分缓解(66.7%),2例患者病灶稳定(13.3%),没有疾病进展的患者。15例患者总有效率为86.7%(13/15)。BED<90Gy的患者有效率为33.3%,而BED>90Gy的患者有效率为100%,差异有统计学意义(P＜0.05)。3例患者出现2级急性放射损伤,未见3级以上毒副反应。1年和2年局控率分别为92.3%和81.2%。结论 SBRT治疗孤立性肺肿瘤可获得较好的近期疗效和局部控制率,急性放射损伤较轻。

Objective To analyze the efficacy of stereotactic body radiation therapy(SBRT) onsolitary pulmonary tumor and explore its clinical value. Methods TOPSLANE X-ray stereotactic body radiation therapy system was used for treatment of fifteen patients with solitary pulmonary tumor, including eight patients with stage I non-small cell lung cancer and seven patients with single lung metastasis. Fraction dose of 5 to 8Gy was prescribed once a day, three times a week, totally 8 to 10 times to a total dose of 50 to 64 Gy and biological effective dose(BED) of 75 to 115Gy. Short-term efficacy, acute radiation injury and local control rate were analyzed. Results After treatment, there were three patients with complete response (CR) (20.0%), ten patients with partial response (PR) (66.7%), tow patients with stable disease (SD) (13.3%), and no patients with progressive disease (PD). The total response rate was 86.7% (13/15). The response rate was 33.3% in patients with BED <90 Gy, while it was 100% in patients with BED> 90 Gy, and the difference was statistically significant (P<0.05). Grade 2 acute radiation injuries were observed in three patients, and no toxicity greater than grade 3 was observed. The 1-year and 2-year local control rate was 92.3% and 81.2%, respectively. Conclusion SBRT on solitary pulmonary tumor has good short-term efficacy and local control rate with mild acute radiation injury.

目的 设计并验证适合Beckman AU5811全自动生化分析仪试剂针和搅拌棒的交叉污染分析方法,找到产生的原因并制定有效的污染避免措施。方法 以反应盘内圈项目(ALT、TBIL、GGT、ALB、TBA、LDH1、CK、Cr、GLU、CO2、TC、HDL-C、APOA1、Ca 、Fe、Cu、HCY 、AFU、PA)作为实验项目,按设计的检测顺序进行交叉污染实验,各项目间的污染率以不超过95%～105%为判断标准。结果 内圈项目中TC、HCL-C对TBA分别存在试剂针和搅拌棒的污染,Ca对Cr存在搅拌棒的污染,Cu对Fe存在试剂针的污染,GGT、ALB、LDH1对CO2都有搅拌棒的污染,TC、TBIL对Cu也存在搅拌棒的污染。结论 采用该方法能有效快速地对Beckman AU5811生化分析仪上的所有项目进行交叉污染实验,确定交叉污染产生的关联项目并采取污染避免措施减少交叉污染,保证检验结果的准确。

Objective To design and verify an analytical method for the cross contamination of Beckman AU5811 automatic biochemical analyzer's reagent needle and stir bar and find the reasons, and make effective measures to avoid contamination. Methods Use the inner ring items (ALT,TBIL,GGT,ALB, TBA,LDH1,CK,Cr,GLU,CO2,TC,HDL-C,APOA1,Ca,Fe,Cu,HCY,AFU,PA) as theexperiment objects and do the cross contamination experiment according to the test order. The standard for the cross contamination level is limited to 95-105% among all items. Results TC and HCL-C were found to have contaminated TBA both in reagent needle and stir bar model, Ca contaminated Cr in the stir bar model; Cu contaminated Fe in the reagent needle model; GGT,ALB and LDH1 contaminated CO2 in stir bar model; TC and TBIL contaminated Cu in the stir bar model. Conclusion The method used in this experiment can effectively identify and analyze the cross contamination of all the items in Beckman AU5811 automatic biochemical analyzer. In addition, it can help to adopt corresponding measures to reduce cross contamination.

目的 调查广州市在校大学生AIDS相关知识及对HIV感染者态度现状,为学校更有效地开展大学生AIDS健康教育提供依据。方法 采用目的抽样方法,对广州市某高校 1200名在校大学生进行关于AIDS相关知识、态度的问卷调查。结果 不同年级、生源地,AIDS相关知识差异均有统计学意义(P值均＜0.01);多数学生对HIV感染者持积极态度,但仅5.73%的学生愿意与HIV感染者共餐;2.70%愿意与感染HIV的同学同住一个寝室。网络是大学生获取AIDS相关知识的最主要途径。结论 大学生AIDS相关知识知晓率水平较高,但缺乏系统性和全面性;多数学生对HIV感染者持积极态度,但是一旦与自我卷入较深,即表现出对HIV感染者的回避甚至歧视。高校应加强对大学生AIDS健康教育的系统性、全面性和深刻性,以消除对AIDS的心理恐惧,达到对HIV感染者的零歧视。

Objective To investigate the AIDS related knowledge of the Guangzhou undergraduates and the attitudes on HIV infection status, for the school to provide basis for AIDS health education and to carry out AIDS health education during college students more effectively. Methods 1200 college students in a university in Guangzhou were surveyed by a questionnaire on HIV/AIDS related knowledge and attitudes, using the way of purposive of sampling. Results There was statistical significant difference on the AIDS-related knowledge in the different grades and origin of students (P<0.01). The majority of students has a positive attitude to the HIV-infected, but only 5.73% of them wer willing to share a meal with HIV-infected persons, and 2.70% of them were willing to live with HIV-infected. The network is the most important way to access to HIV/AIDS related knowledge for college students. Conclusion The level awareness during college student on AIDS-related knowledge is high, but lack of systematic and comprehensive. The majority of students had a positive attitude towards the HIV-infetected, however once self-involved, they may avoid even discriminate the HIV-infected individuals. Therefore, the universities should strengthen AIDS health education of college students comprehensively and profoundly, in order to eliminate fear of AIDS, and reached zero discrimination HIV-infected persons in the future.

目的 对86例青春期多囊卵巢综合症(polycystic ovary syndrome,PCOS)患者进行随访,探讨患者长期治疗的依从性。方法 选择2009年11月—2012年6月在我院门诊首次诊断为青春期PCOS患者进行前瞻性队列研究。按随诊方法不同分为常规随访组和可及与连贯病人服务(ACC)组,前瞻随访6个月。分别在初次就诊时、3个月及6个月后对比两组的身体质量指数(Body Mass Index, BMI)、复诊率和遵医嘱服药率。结果 共86名患者入组,其中常规随访组43人,ACC组43人。两组基线资料基本一致。随访3月时,两组的BMI、复诊率和遵医嘱服药率的差异无统计学意义(P>0.05)。随诊6月时,ACC组的复诊率和服药依从性均优于常规随访组分别为(97.7% 比 81.4%,97.7% 比 76.7%,P＜0.05),BMI比常规随访组下降(22.33±7.31 比 24.59±7.8 kg/m²,t<0.05)。结论 可及与连贯的病人服务可以改善青春期PCOS患者BMI及长期随诊的复诊率和服药依从性。

Objective To investigate the compliance of adolescent patients with polycystic ovary syndrome (PCOS) in long-term treatment by access to care and continuity of care(ACC) on 86 cases. Methods A prospective cohort study was taken for 6 months on adolescent patients diagnosed as PCOS for the first time in out-patient department from November 2009 to June 2012. These patients were selected and divided into ACC group and regular follow-up group according to the different modes of follow-up visit. The relevant data including body mass index (BMI), clinic visit rate and rate of medication adherence were compared in the first visit and 3rd, 6th month in the follow-up visit. Results 86 patients were enrolled with 43 in ACC group and 43 in regular follow-up group. The baseline information was similar between the two groups. There were no statistically significant difference between groups in BMI, clinic visit rate and rate of medication adherence after 3 months (P>0.05). However, after 6 months, BMI, clinic visit rate and rate of medication adherence were significantly improved in the ACC group compared to the regular follow-up group (22.33±7.31 vs 24.59±7.8 kg/m², 97.7% vs 81.4%, 97.7% vs 76.7%, P＜0.05, respectively). Conclusion ACC could improve BMI, clinic visit rate and medication adherence of adolescent patients with polycystic ovary syndrome in long-term treatment.

目的 获得广东省保健食品违法广告所针对目标消费人群的现况,为加强保健食品广告的管理提供研究基础。方法 将2009年1月—2014年10月广东省食品药品监督管理局官方网站公布总计40期保健食品违法广告的公告汇总,以目标消费群体的年龄性别作为分析因素,建立统计表进行相关研究分析。结果 2009年1月—2014年10月广东省保健食品违法广告主要是针对中老年人消费人群,并侧重于女性消费群体。结论 针对广东省保健食品违法广告存在问题,相关职能部门应增补保健品广告法规的操作细则,加强部门间协调分工及相互监督,并利用网络媒体平台开展相关保健食品知识宣传工作。

Objective This study the characters of consumers targeted by health food advertisements in Guangdong province. The results will be helpful to enhance the standard management to the advertisements of health food in Guangdong Province. Methods Forty issues announcements of illegal health food advertisements, which published in the official website of Guangdong food and drug administration center from January 2009 to October 2014, were collected and statistically analyzed by the age and gender of the target consumer groups. Results The old people, especially female consumers, were the target consumers of illegal health food advertisements in Guangdong province from January 2009 to October 2014. Conclusion Regulations and rules for the health food advertisements should be developed. And the coordination and supervision among advertizing departments, as well as knowledge to the health food advertisements based on the internet technique, should be strengthened.

目的 探讨COOK双球囊导管和欣普贝生引产的优劣性。方法 选择住院分娩的孕妇388例,根据引产方式分为两组,研究组应用COOK双球囊导管,对照组应用欣普贝生。根据宫颈Bishop评分,研究组分为研究组1和研究组2,对照组分为对照组1和对照组2,研究组1和对照组1宫颈Bishop评分小于或等于3分,研究组2和对照组2宫颈Bishop评分大于3分而小于或等于6分。分别对各组促宫颈成熟及引产情况、妊娠结局进行比较。结果 各组放置COOK宫颈双球囊或欣普贝生前后,宫颈Bishop评分有差异,低宫颈Bishop评分组欣普贝生效果更好。研究组与对照组比较,阴道分娩和剖宫产病例差异无统计学意义;急产、产后出血、羊水污染病例差异有统计学意义。研究组新生儿窒息率低于对照组,体温>37.5℃发生率高于对照组,差异无统计学意义。结论 对宫颈条件较差者,建议选用欣普贝生诱导宫颈成熟;COOK双球囊导管引产,作用温和,安全性好,成功率高。

Objective To evaluate the advantage of the COOK double balloon catheter and Propess induced labor. Methods We collected 388 cases with pregnant women. The patients were divided into the research and control groups. The research group applied COOK double balloon catheter while the control group applies Propess. According to cervical Bishop rating, the research team was divided into research group 1 and research group 2. The control group was divided into control group 1 and the control group 2. The cervical Bishop score of the research group 1 and control group 1 was less than or equals 3 points. The cervical Bishop score of the research group 2 and the control group 2 was greater than 3 and less than or equal 6 points. We separately compared the pregnancy outcome for each group to promote cervical mature and induced labor situation. Results The cervical Bishop scores have significant difference before and after COOK cervical double balloon and Propess are positioned between two groups.The effect of low cervical Bishop scores group is better than the high cervical Bishop by propess. The rates of urgent production, postpartum hemorrhage and amniotic fluid pollution reduce significant in the research group. The neonatal asphyxia rate, incidence of temperature > 37.5, vaginal delivery cases and cesarean section cases have not significant differences between the research group and the control group. Conclusion We suggest that patients whose cervical condition is poor should choose Propess induced cervical mature; The COOK double balloon catheter induced labor is safe and has a high success rate.

神经元特异性烯醇化酶(neuron-specific enolase, NSE)是糖酵解途径中一种重要的同工酶;特异性位于神经元和神经内分泌细胞中。小细胞肺癌(small cell lung cancer,SCLC)细胞浆内含有神经内分泌颗粒,具有神经内分泌分化的特征,为恶性程度高的神经内分泌系统肿瘤。因此,NSE是SCLC诊断中最敏感的肿瘤标志物,在SCLC的临床诊断、治疗、预后均有重要应用价值,科学合理联合检测肿瘤标记物,将能为临床诊疗工作提供有力的帮助。

目的 了解CHPS患儿经胃镜下幽门肌切开术治疗后幽门部黏膜近期各种并发症的发生情况及对预后的影响。方法 选取2006—2012年我院新生儿科确诊的34例CHPS患儿。以超细胃镜进入十二指肠乳头处,使用电切刀按上行性方向,沿幽门管纵轴切割肥厚狭窄的幽门管黏膜及环形肌以解除幽门部梗阻。术后第1、3、6月复查胃镜。结果 34例CHPS患儿共行胃镜下幽门肌切开术48次,均未发生胃及幽门穿孔、撕裂等严重并发症。24例患儿术后通过胃镜进行了复查,胃镜见幽门部及黏膜改变主要有黏膜充血肿胀、切口未完全愈合、切口疤痕形成、幽门部息肉形成等。结论 经胃镜下幽门肌切开术是一种安全性较高的治疗CHPS的新技术。术后黏膜充血肿胀、切口未完全愈合等幽门部黏膜改变的发生率随时间下降。切口疤痕形成并未影响幽门功能。

目的 探讨用捏脊联合中药外洗治疗脾胃气虚型儿童功能性消化不良的效果。方法 随机选取脾胃气虚型功能性消化不良的儿童120例,分为联合组、捏脊组、外洗组、对照组4个组,每组30例,分别采用捏脊、中药外洗、捏脊联合中药外洗、吗丁啉进行治疗,两周后观察4组治疗效果及唾液淀粉酶活性比值、D-木糖排泄率的变化,进行分析。结果 联合组的有效率优于捏脊组、外洗组、对照组,差异有统计学意义(P<0.05)。治疗后联合组的唾液淀粉酶活性比值比捏脊组、外洗组、对照组提高,差异有统计学意义(P<0.05)。治疗后D-木糖排泄率均较治疗前有增加,两两比较,联合组与对照组比较差异有统计学意义(P<0.05)。结论 捏脊联合中药外洗可改善脾胃气虚型儿童功能性消化不良,疗效良好,其机理同改善唾液淀粉酶活性有关。

目的 分析乳腺癌细胞中Snail与MTDH基因的作用,明确Snail是否通过结合于MTDH的启动子区域促进乳腺癌转移。方法 克隆、转染Snail基因至乳腺癌细胞,观察过表达Snail的乳腺癌细胞中MTDHmRNA及蛋白表达的变化;再使用免疫共沉淀法检测Snail与MTDH基因的共作用。结果 转染Snail基因进入乳腺癌MDA-MB-435细胞后,转染组、空白组和对照组中MTDHmRNA的表达水平分别为1.61±0.22、1.02±0.18、0.99±0.20,转染组高于空白组和对照组,差异有统计学意义(P<0.05),而后两组表达无差异(P>0.05);Westren blot检测结果显示,Snail可促进MTDH蛋白的表达;免疫共沉淀显示,Snail与MTDH在细胞内存在相互结合作用。结论 Snail在乳腺癌细胞中可通过结合于MTDH基因的启动子区域,促进MTDHmRNA转录及相关蛋白的表达,从而导致乳腺癌转移。

Objective To investigate the function of Snail to MTDH gene in breast cancer cells. Methods We observed the changement of MTDHmRNA and protein expression in breast cancer cell line MDA-MB-435 after transfected with Snail gene. Then, we used co-immunoprecipitation to determine the domain of Snail and MTDH binding in vitro. Results After transfected with Snail gene into MDA-MB-435 cell, the expression levels of MTDHmRNA in transfection group, blank group and control group were 1.61±0.22,1.02±0.18,0.99±0.20. The level of transfection group was significantly higher than the other groups(P< 0.05). Western blot showed that the expression of MTDH protein can be promoted by Snail. Co-immunoprecipitation showed that Snail and MTDH are binding interactions in breast cancer cell line MDA-MB-43. Conclusion Snail can promote transcription and expression of MTDH in breast cancer cells by binding to the promoter region of the MTDH gene resulting in metastasis of breast cancer.