目的 评价实时荧光定量PCR分析低氧性肺动脉高压大鼠肺动脉平滑肌基因表达时12个候选内参基因表达的稳定性,获得最适合的内参基因。方法 以低氧性肺动脉高压大鼠肺动脉平滑肌为研究对象,选择文献报道的常用12种内参基因为候选内参基因,利用geNorm和NormFinder程序分析实时荧光定量PCR数据,筛选出最适内参基因。结果 12个候选内参基因在低氧性肺动脉高压大鼠肺动脉平滑肌表达稳定性由强到弱顺序为:TBP>B2M>HPRT1>HMBS>RPL13a>18sRNA>PPIA>ACTB>GUSB>TFRC>GAPDH>PGK1,平均表达稳定度(M值)均<0.5,geNorm和NormFinder评估后推荐使用TBP和B2M一起作为该研究时的内参基因。结论 同时使用TBP和B2M是实时荧光定量PCR分析低氧性肺动脉高压大鼠肺动脉平滑肌基因表达的最适合内参基因,为低氧性肺动脉高压相关基因研究提供最优内参基因。
Objective To compare and select the suitable reference genes in real-time quantitative PCR analysis of rat pulmonary artery smooth muscle cells mRNA expression level of pulmonary hypertension. Methods To choose appropriate reference gene, the expression of twelve commonly use housekeeping genes were examined in rat pulmonary artery smooth muscle cells of hypoxia-induced pulmonary hypertension by using geNorm and NormFinder programs. Results The expression consistency of 12 genes was (from high to low): TBP>B2M>HPRT1>HMBS>RPL13a>18sRNA>PPIA>ACTB>GUSB>TFRC>GAPDH>PGK1. The average expression stability(M) values of them were low than 0.5. TBP and B2M reference genes were recommended to use in the same condition. Conclusion TBP and B2M reference genes were the most suitable combination of the reference genes for real-time quantitative PCR analysis in rat pulmonary artery smooth muscle cells of hypoxia-induced pulmonary hypertension.
目的 了解青海省北京/W系结核分枝杆菌分布特征。方法 收集青海地区结核分支杆菌临床分离株,采用RD105缺失基因检测鉴定北京/W系结核分枝杆菌。结果 共收集237株结核分枝杆菌临床分离株,采用RD105缺失基因检测鉴定北京/W系结核分枝杆菌220株,占92.8%,非北京/W结核分枝杆菌,共17株,占7.2%。北京/W系结核分枝杆菌在青海地区性别与民族分布差异没有统计学意义(P＞0.05)。结论 北京/W结核分枝杆菌为青海地区流行菌株,在人群易于发生感染和传播。
Objective To ascertain the epidemiological characteristics of Beijing/W lineage Mycobacterium tuberculosis in Qinghai Province. Methods M. tuberculosis clinical isolates were collected and identified with an RD105 deletion test.Statistical analysis was performed by using the test. Results Totally, 237 clinical isolates of M. tuberculosis were collected in which 220 strains (92.8%) belonged to the Beijing/W lineage of M. tuberculosis while 17strains (7.2%) belonged to the non-Beijing/W lineage of M. tuberculosis according to the RD105 deletion test. There were no significant differences in the distribution of Beijing/W lineage of M. tuberculosis in the gender and nationality (P＞0.05). Conclusion Beijing/W lineage of M. tuberculosis were prevalent in Qinghai province and prone to having infection and transmission in the crowd.
目的 探讨二维超声联合三维超声检测不同孕期胎儿肢体畸形的临床价值。方法 回顾性分析我科2013年1月—2015年1月超声筛查的1352名孕15~34周的孕妇,运用二维及三维超声,采用连续顺序追踪超声法检测胎儿四肢,将产前诊断结果与引产或引产结果对照。结果 1352名孕妇胎儿畸形46例(3.40%,46/1352),总涉及肢体73处(近端肢体畸形65处,远端肢体畸形8处),其中四肢短小9例(骨发育不全2例,单纯四肢短小畸形5例,成骨发育不全2例),漏诊2例;足畸形16例34处,漏诊1例,残肢畸形15例;手畸形6例,漏诊1例。结论 孕中期二维连续顺序追踪超声法联合三维超声胎儿肢体畸形检出率高,对及早发现胎儿肢体畸形有一定的诊断价值。
目的 了解河源市无偿献血者的人群结构特征及其与血液检测结果的关系,为完善该地区安全血源招募和献血者筛查策略提供依据。方法 收集2014年1月1日—2014年12月31日该市15184例首次无偿献血者登记表及检测结果,统计分析无偿献血者的年龄、性别、职业、文化程度等分布情况及其血液检测结果不合格率。结果 该地区无偿献血者以男性(78.91%)、年龄以35岁以下(63.44%)、在职职员(34.08%)、大学专科以上文化程度(39.59%)为主体;献血模式以个人自愿(53.58%)和团体自愿(38.41%)为主。该市无偿献血者血液检测结果总体不合格率为4.29%,其中不同性别、年龄、职业、文化程度、献血模式的无偿献血者组间不合格率差异有统计学意义(χ2值分别为 19.079、38.103、61.042、41.191、44.079,P值均<0.05);多因素Logistic 回归分析显示性别、年龄、职业、文化程度和献血模式为血液检测结果不合格率的主要影响因素。结论 应根据无偿献血者人群结构特点有效开展献血知识宣传教育,认真做好献血前筛查。
目的 探讨单克隆免疫球蛋白血症患者M蛋白质量浓度检测的临床意义。方法 选取2018年6月—2023年6月龙岩人民医院收治的88例单克隆免疫球蛋白血症患者为研究对象，其中意义未明单克隆免疫球蛋白血症（MGUS）21例，具有肾脏意义单克隆免疫球蛋白血症（MGRS）50例，血液系统恶性肿瘤17例。对比其M蛋白质量浓度及临床实验室相关指标表达水平，采用Spearman相关分析法分析临床实验室相关指标的与M蛋白的相关性，对所有患者进行半年随访，以预后情况作为因变量，纳入Logistics回归模型分析M蛋白质量浓度对单克隆免疫球蛋白血症预后的预测价值。结果 不同病种M蛋白水平分别为（2.42±0.55）（2.57±0.64）（4.36±0.64）g/L、24 h尿蛋白分别为（1.45±0.16）（2.98±0.68）（2.43±0.44）g/24 h、血清白蛋白质量浓度分别为（31.01±3.06）（35.03±5.04）（39.05±7.08）g/L、总胆固醇水平分别为（3.42±1.25）（3.87±0.64）、（4.16±0.64）mmol/L、血肌酐水平分别为（114.35±23.23）（81.18±12.12）（146.36±21.12）μmol/L、血红蛋白质量浓度分别为（148.12±15.26）（141.69±12.15）（133.34±15.31）g/L，组间对比差异均有统计学意义（F分别为23.890，19.700，12.044，25.767，36.572，10.267，P＜0.05）。MGUS患者24h尿蛋白与M蛋白有相关性（r=-0.384，P=0.033），24 h尿蛋白、血清白蛋白、总胆固醇、血肌酐与MGRS患者M蛋白有相关性（r=-0.586，P=0.006；r=0.431，P=0.018；r=-0.457，P=0.020；r=0.523，P=0.009），血清白蛋白、总胆固醇、血红蛋白与血液系统恶性肿瘤患者M蛋白有相关性（r=0.374，P=0.029；r=-0.617，P=0.001；r=-0.414，P=0.024）；年龄、M蛋白为单克隆免疫球蛋白血症患者预后的影响因素（P＜0.05）。结论 不同单克隆免疫球蛋白血症患者M蛋白水平存在差异，其中血液系统恶性肿瘤患者的M蛋白水平最高，且M蛋白为单克隆免疫球蛋白血症预后的独立影响因素。
Objective To explore the clinical significance of detecting M protein concentration in patients with monoclonal gammopathy．Methods From June 2018 to June 2023，88 patients with monoclonal gammopathy admitted to the hospital were selected as the study subjects．Among them，21 cases of monoclonal gammopathy with undetermined significance（MGUS），50 cases of monoclonal gammopathy with renal significance（MGRS），and 17 cases of hematological malignancies were selected．Concentration of M protein and the expression levels of clinical laboratory related indicators were compared，Spearman correlation analysis was used to analyze the correlation between clinical laboratory related indicators and M protein．All patients were followed up for six months，with prognosis as the dependent variable，included in the logistic regression model to analyze the predictive value of M protein concentration on the prognosis of monoclonal gammopathy．Results There were significant differences in the expression levels of M protein（［2.42±0.55］，［2.57±0.64］，［4.36±0.64］）g/L，24-hour urine protein（［1.45±0.16］，［2.98±0.68］，［2.43±0.44］）g/24 h，serum albumin（［31.01±3.06］，［35.03±5.04］，［39.05±7.08］）g/L，total cholesterol（［3.42±1.25］，［3.87±0.64］，［4.16±0.64］）mmol/L，blood creatinine（［114.35±23.23］，［81.18±12.12］，［146.36±21.12］）μmol/L，and hemoglobin（［148.12±15.26］，［141.69±12.15］，［133.34±15.31］）g/L among different diseases（F=23.890，19.700，12.044，25.767，36.572，10.267；P＜0.05）．There was a significant correlation between 24 h urinary protein and M protein in MGUS patients（r=-0.384，P=0.033）．Urinary protein，serum albumin，serum cholesterol and blood creatinine were significantly associated with M protein in MGRS patients（r=-0.586，P=0.006；r=0.431，P=0.018；r=-0.457，P=0.020；r=0.523，P=0.009），Serum albumin，total cholesterol，and hemoglobin were significantly associated with M protein in patients with hematological malignancies（r=0.374，P=0.029；r=-0.617，P=0.001；r=-0.414，P=0.024；P＜0.05）．Age and M protein were independent risk factors for the prognosis of patients with monoclonal gammopathy（P＜0.05）．Conclusions There are significant differences in the concentration of M protein among patients with different levels of monoclonal gammopathy，with the highest level observed in patients with hematological malignancies．M protein is an independent prognostic factor for monoclonal gammopathy．
