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2023年7月 第38卷 第7期11
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TAK1与TCF-4在非小细胞肺癌中表达及其相关性的初步研究

Preliminary study on the expression and correlation of TAK1 and TCF-4 in non-small cell lung cancer

来源期刊: 广州医药 | 17-22 发布时间:2023-07-27 收稿时间:2025/11/13 18:33:16 阅读量:33
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关键词:
非小细胞肺癌转化生长因子激活激酶1T细胞因子-4Wnt/β-catenin信号通路
NSCLCTAK1TCF-4Wnt/β-catenin signaling pathway
DOI:
10.3969/j.issn.1000-8535.2023.06.003
收稿时间:
2022-08-29 
修订日期:
 
接收日期:
 
引用总数:
4  
目的 探讨非小细胞肺癌(NSCLC)组织中转化生长因子β激活激酶1(TAK-1)与T细胞因子-4(TCF-4)在 mRNA以及蛋白水平的表达情况及其相关性,并分析两者与NSCLC患者临床病理因素的关系。方法 收集NSCLC手术标本51例,每例均包含肺癌组织及配对癌旁组织,所有患者的术后诊断均经病理结果证实,通过RT-PCR以及Western blot法检测TAK1、TCF-4在癌组织及配对癌旁组织中的表达情况,并通过SPSS进一步分析两者的相关性及其与临床病理因素的关系。结果 TAK1与TCF-4 mRNA以及蛋白水平在NSCLC患者癌组织中均高表达,其中TAK1蛋白的表达与NSCLC的TNM分期(P=0.022)、淋巴结转移(P=0.014)相关,TCF-4蛋白的表达与NSCLC的TNM分期相关(P=0.045)。TAK1在NSCLC组织中的表达与TCF-4呈正相关(r=0.427,P=0.002)。结论 TAK1 mRNA及蛋白水平在NSCLC组织中均高表达,并与TCF-4呈正相关,TAK1有可能成为NSCLC诊断及预后的一个潜在靶标,并且TAK1与TCF-4的联合应用有可能成为一种更为理想的NSCLC辅助诊断及临床治疗方法。
Objective To investigate the mRNA and protein expressions of transforming growth factor β-activated kinase 1(TAK1)and T cell factor-4(TCF-4)in non-small cell lung cancer(NSCLC)tissues and their correlation,and to analyze the relationship between TAK1/TCF-4 and clinicopathological factors in NSCLC patients.Methods Cancer tissues and matched adjacent tissues of 51 NSCLC patients in our hospital were collected.The postoperative diagnosis of all patients was confirmed by pathological results.The expression of TAK1 and TCF-4 in cancer tissues and paired adjacent tissues were detected by RT-PCR and Western blot,then SPSS was used to further analyze the correlation between TAK1 and TCF-4 and clinicopathological factors.Results TAK1 and TCF-4 mRNA and protein were highly expressed in NSCLC tissues,and TAK1 protein expression was significantly correlated with TNM stage of NSCLC(P=0.022)and lymph node metastasis(P=0.014);TCF-4 protein expression was significantly correlated with TNM stage of NSCLC(P=0.045).TAK1 expression in NSCLC tissues was positively correlated with TCF-4(r=0.427,P=0.002).Conclusions TAK1 mRNA and protein were highly expressed in NSCLC tissues and positively correlated with TCF-4.TAK1 may become a potential target for the diagnosis and prognosis of NSCLC,and the combined application of TAK1 and TCF-4 may become a more ideal method for the auxiliary diagnosis and clinical treatment of NSCLC.
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