您的位置: 首页 > 2020年11月 第51卷 第6期 > 文字全文
2023年7月 第38卷 第7期11
目录

叶酸下调子宫内膜癌中NRBP2基因的表达

Folic acid down-regulates NRBP2 gene expression in endometrial cancer

来源期刊: 广州医药 | 19-23 发布时间:2021-11-28 收稿时间:2025/11/13 17:39:21 阅读量:32
作者:
关键词:
子宫内膜癌叶酸转录组测序
Endometrial cancerFolic acidTranscriptome sequencing
DOI:
10.3969/j.issn.1000-8535.2020.06.004
收稿时间:
2020-02-24 
修订日期:
 
接收日期:
 
引用总数:
0  
目的 探究叶酸对子宫内膜癌作用的靶基因。方法 通过转录组测序筛选叶酸作用下子宫内膜癌细胞中的差异基因,生存分析寻找对子宫内膜癌具有生存意义的差异基因,qPCR及western blot检测其在叶酸作用下的表达。结果 转录组测序发现36个差异基因,生存分析发现FMN1,TRIB3,INHBE及NRBP2的表达对子宫内膜癌具有生存意义,qPCR及western blot验证叶酸作用下NRBP2在子宫内膜癌细胞中的表达下调。结论 叶酸下调子宫内膜癌中NRBP2基因的表达,NRBP2可能是叶酸对子宫内膜癌作用的靶标。
Objective To explore the target genes of folic acid on endometrial carcinoma. Methods The differential genes in endometrial cancer cells treated with folic acid were screened by transcriptome sequencing. Survival analysis was used to find the differential genes with survival significance. QPCR and western blot were used to detect their expression under the action of folic acid. Results 36 differential genes were found by transcriptional sequencing. Survival analysis showed that the expression of FMN1,TRIB3,INHBE and NRBP2 had survival significance in endometrial carcinoma. QPCR and western blot confirmed that the expression of NRBP2 in endometrial cancer cells was down-regulated by folic acid. Conclusion Folic acid down-regulates the expression of NRBP2 gene in endometrial carcinoma, and NRBP2 may be the target of the effect of folic acid on endometrial carcinoma.
1、 QU J, LIU B, LI B, et al. TRIB3 suppresses proliferation and invasion and promotes apoptosis of endometrial cancer cells by regulating the AKT signaling pathway[J]. Onco Targets Ther, 2019(12):2235-2245. QU J, LIU B, LI B, et al. TRIB3 suppresses proliferation and invasion and promotes apoptosis of endometrial cancer cells by regulating the AKT signaling pathway[J]. Onco Targets Ther, 2019(12):2235-2245.
2、 JUCKSTOCK J, KIMMICH T, MYLONAS I, et al. The inhibin-betaC subunit is down-regulated, while inhibin-betaE is up-regulated by interferon-beta1a in Ishikawa carcinoma cell line[J]. Arch Gynecol Obstet, 2013, 288(4):883-888. JUCKSTOCK J, KIMMICH T, MYLONAS I, et al. The inhibin-betaC subunit is down-regulated, while inhibin-betaE is up-regulated by interferon-beta1a in Ishikawa carcinoma cell line[J]. Arch Gynecol Obstet, 2013, 288(4):883-888.
3、 KIMMICH T, BRUNING A, KAUFL S D, et al. Inhibin/activin-beta C and -beta E subunits in the Ishikawa human endometrial adenocarcinoma cell line[J]. Arch Gynecol Obstet, 2010, 282(2):185-191. KIMMICH T, BRUNING A, KAUFL S D, et al. Inhibin/activin-beta C and -beta E subunits in the Ishikawa human endometrial adenocarcinoma cell line[J]. Arch Gynecol Obstet, 2010, 282(2):185-191.
4、 ZHANG L, GE C, ZHAO F, et al. NRBP2 Overexpression increases the chemosensitivity of hepatocellular carcinoma cells via akt signaling[J]. Cancer Res, 2016, 76(23):7059-7071. ZHANG L, GE C, ZHAO F, et al. NRBP2 Overexpression increases the chemosensitivity of hepatocellular carcinoma cells via akt signaling[J]. Cancer Res, 2016, 76(23):7059-7071.
5、 LIU J J, HAZRA A, GIOVANNUCCI E, et al. One-carbon metabolism factors and endometrial cancer risk[J]. Br J Cancer, 2013, 108(1):183-187. LIU J J, HAZRA A, GIOVANNUCCI E, et al. One-carbon metabolism factors and endometrial cancer risk[J]. Br J Cancer, 2013, 108(1):183-187.
6、 DU L, WANG Y, ZHANG H, et al. Folate intake and the risk of endometrial cancer: A meta-analysis[J]. Oncotarget, 2016, 7(51):85176. DU L, WANG Y, ZHANG H, et al. Folate intake and the risk of endometrial cancer: A meta-analysis[J]. Oncotarget, 2016, 7(51):85176.
7、 LU J, TRABERT B, LIAO L M, et al. Dietary intake of nutrients involved in folate-mediatedone-carbon metabolism and risk for endometrial cancer[J]. Int J Epidemiol, 2019, 48(2):474-488. LU J, TRABERT B, LIAO L M, et al. Dietary intake of nutrients involved in folate-mediatedone-carbon metabolism and risk for endometrial cancer[J]. Int J Epidemiol, 2019, 48(2):474-488.
8、 刘颖慧,黎金颜,何淑明,等. 不同分型分期子宫内膜癌叶酸受体α的表达及诊断性能评估[J]. 实用医学杂志, 2018, 34(4):592-595. 刘颖慧,黎金颜,何淑明,等. 不同分型分期子宫内膜癌叶酸受体α的表达及诊断性能评估[J]. 实用医学杂志, 2018, 34(4):592-595.
9、 NEWMAN A C, MADDOCKS O. One-carbon metabolism in cancer[J]. Br J Cancer, 2017, 116(12):1499-1504. NEWMAN A C, MADDOCKS O. One-carbon metabolism in cancer[J]. Br J Cancer, 2017, 116(12):1499-1504.
上一篇
下一篇
出版者信息








《广州医药》公众号
目录