Objective To study the antibacterial effect of the purple tiger burn ointment. Methods The MIC and MBC were determined by using in vitro test. Comparison of the inhibition zone size of the different representative strains of the purple tiger burn ointment and the moist burn ointment were made, the animal experiment was used to compare the colony count of the wound of the rat model of second degree scald after 24 h, 48 h with the purple tiger burn ointment and the moist burn ointment. Results MIC(Minimum inhibitory concentration)of purple tiger burn ointment on Staphylococcus aureus was 8 mg/mL, MBC(Minimum bactericidal concentration ) of purple tiger burn ointment on Staphylococcus aureus was 32 mg/mL; MIC of purple tiger burn ointment on Pseudomonas aeruginosa was 16 mg/mL, MBC of purple tiger burn ointment on Pseudomonas aeruginosa was 32 mg/mL, MIC of purple tiger burn ointment on Escherichia coli was 16 mg/mL, MBC of purple tiger burn ointment on Escherichia coli was 64 mg/mL. The results showed that there was no significant difference between the purple tiger burn ointment and the moist burn ointment. Animal experiments showed that the purple tiger burn ointment and the moist burn ointment had different degrees of inhibition of the role of local bacteria in the second degree scald in rats. Purple tiger burn cream showed stronger function. The longer the time took, the more obvious the antibacterial effect was. Conclusion The purple tiger burn ointment can effectively inhibit the second degree burn of local bacteria, prevent the wound infection and protect the wound, so as to play a role in the treatment of second degree scald.