Objective To analyze the relative factors which influence the clinical pregnancy rates of patients accepted intrauterine insemination with husband's sperm. Methods 225 cases of infertile patients accepted IUI treatment were selected, 400 cycles were included and the clinical data were analyzed retrospectively. Observing the relationship between the age of women, treatment options, endometrial thickness on hCG injection day, cycles of IUI and pregnancy rates. Results The clinical pregnancy rates of women less than 30 years old(21.74%) were higher than aged between 30 to 38 years old(17.48%) and more than aged 38 years old(8.22%), P<0.05.The clinical pregnancy rates of ovulation induction options(CC、HMG、CC+HMG) were 19.05%, 19.66% and 14.71%, higher than the pregnancy rates of natural cycle 7.14% significantly, P<0.05. The pregnancy rates of the group of endometrial thickness ≥8mm on hCG injection day were 23.56%, higher than the group of endometrial thickness <8 mm 13.27%, P<0.05. The clinical pregnancy rates of 1 to 5 cycles IUI were 21.30%, 15.60%, 9.38%, 0% and 0% respectively, the difference of repeating the IUI cycles’ number was statistical significance (P<0.05). Conclusion The ages of women, endometrial thickness on hCG injection days and treatment options can affect the clinical pregnancy rates. Extending the number of IUI treatment cycles can not increase the pregnancy rates of IUI. All the factors should be comprehensive to assess the patient's pregnancy rates again, to find more reasons further for the failure of assisted reproduction or turn to IVF-ET assisted reproduction treatment when it is necessary.