您的位置: 首页 > 2016年7月 第47卷 第4期 > 文字全文
2023年7月 第38卷 第7期11
目录

Fmr1基因敲除小鼠悬尾实验的观察

Observation of tail suspension test in Fmr1 gene knockout mice

来源期刊: 广州医药 | 9-11 发布时间:2021-12-02 收稿时间:2025/11/13 16:57:14 阅读量:12
作者:
关键词:
悬尾实验脆性X综合征抑郁行为
Fragile X SyndromeTail suspension testDepressive behavior
DOI:
10.3969/j.issn.1000-8535.2016.04.003
收稿时间:
2016-04-01 
修订日期:
 
接收日期:
 
引用总数:
0  
目的 对不同周龄的KO小鼠与WT小鼠进行悬尾实验进行观察,探讨KO小鼠与WT小鼠的行为差别。方法 采用健康的试验动物180只分两组:①KO组(4、6、8周龄,各周龄30只,雌雄各半,共90只)②WT组(4、6、8周龄,各周龄30只,雌雄各半,共90只);通过悬尾实验观察性别,年龄对不动时间的影响。结果 同龄KO雌性小鼠比雄性小鼠的静止时间差别不大;随着年龄增大,静止时间增长。 同龄同性别的KO鼠比WT鼠的不动时间长。P<0.05;同龄雄性小鼠比雌性小鼠的不动时间短; 随年龄增长各种系小鼠不动时间增长,KO鼠的不动时间比WT鼠长,P<0.05。结论 KO小鼠存在抑郁行为表型。
Objective To observe tail suspension test in Fmr1 gene knockout mice and to explore whether there are differences in mobility of KO and WT mice. Methods 180 test mice were divided into two groups: ① KO group (4,6,8 weeks old, each age group of mice is 30, male and female in half, a total of 90) ② WT group (4,6, 8 weeks old, each group of mice is 30, male and female on half, a total of 90). Through forced swimming test and tail suspension test to observe gender, age effect on immobility time. Results With the same age of the same sex,the KO mice's immobility time was longer than WT mice's. P<0.05. With the same age,the male mice's immobility time was shorter than female mice's. With the age increase, the immobility time of KO mice was longer than WT mice. P<0.05. Conclusion Fmr1 gene knockout mice have anxiety and depressive behavior.
1、 THOMAS AM, BUI N, GRAHAM D, et al. Genetic reduction of group 1 metabotropic glutamate receptors alters select behaviors in a mouse model for fragile X syndrome[J]. Behav Brain Res, 2011,223(2): 310-321. THOMAS AM, BUI N, GRAHAM D, et al. Genetic reduction of group 1 metabotropic glutamate receptors alters select behaviors in a mouse model for fragile X syndrome[J]. Behav Brain Res, 2011,223(2): 310-321.
2、 MARKO UUTELA, JESSE LINDHOLM, TOMIRANTAM?KI, et al. Distinctive behavioral and cellular responses to fluoxetine in the mouse model for Fragile X syndrome[J]. Cell Neurosci,2014,8: 150. MARKO UUTELA, JESSE LINDHOLM, TOMIRANTAM?KI, et al. Distinctive behavioral and cellular responses to fluoxetine in the mouse model for Fragile X syndrome[J]. Cell Neurosci,2014,8: 150.
3、 PRESCOTT TL, HAYES J, PRIDE M, et al. Normal performance of fmr1 mice on a touchscreen delayed nonmatching to position working memory task[J/OL]. eNeuro,2016,3(1).[2016-04-25] http://europepmc.org/articles/PMC4800045.DOI:10.1523/ENEURO.0143-15.2016. PRESCOTT TL, HAYES J, PRIDE M, et al. Normal performance of fmr1 mice on a touchscreen delayed nonmatching to position working memory task[J/OL]. eNeuro,2016,3(1).[2016-04-25] http://europepmc.org/articles/PMC4800045.DOI:10.1523/ENEURO.0143-15.2016.
4、 CHRISTOPHER J. YUSKAITIS, MARJELO A,et al. Lithium ameliorates altered glycogen synthase kinase-3 and behavior in a mouse model of Fragile X syndrome[J]. Biochem Pharmacology, 2010,79(4):632-646. CHRISTOPHER J. YUSKAITIS, MARJELO A,et al. Lithium ameliorates altered glycogen synthase kinase-3 and behavior in a mouse model of Fragile X syndrome[J]. Biochem Pharmacology, 2010,79(4):632-646.
5、 ABOU-ELNAGA AF, TORIGOE D, FOUDA MM, et al. Identification of multiple genetic loci in the mouse controlling immobility time in the tail suspensionand forced swimming tests[J]. Jpn J Vet Res, 2015, 63(2):53-62. ABOU-ELNAGA AF, TORIGOE D, FOUDA MM, et al. Identification of multiple genetic loci in the mouse controlling immobility time in the tail suspensionand forced swimming tests[J]. Jpn J Vet Res, 2015, 63(2):53-62.
6、 PINTO BROD LM, FRONZA MG, VARGAS JP. Involvement of monoaminergic system in the antidepressant-like effect of (octylseleno)-xylofuranoside in the mouse tail suspension test[J]. Prog Neuropsychopharmacol Biol Psychiatry,2016,65:201-207. PINTO BROD LM, FRONZA MG, VARGAS JP. Involvement of monoaminergic system in the antidepressant-like effect of (octylseleno)-xylofuranoside in the mouse tail suspension test[J]. Prog Neuropsychopharmacol Biol Psychiatry,2016,65:201-207.
7、 KORDJAZY N, HAJ-MIRZAIAN A, AMIRI S. Involvement of N-methyl-d-aspartate receptors in the antidepressant-like effect of 5-hydroxytryptamine 3 antagonists in mouse forced swimming test and tail suspension test[J]. Pharmacol Biochem Behav,2016,141:1-9. KORDJAZY N, HAJ-MIRZAIAN A, AMIRI S. Involvement of N-methyl-d-aspartate receptors in the antidepressant-like effect of 5-hydroxytryptamine 3 antagonists in mouse forced swimming test and tail suspension test[J]. Pharmacol Biochem Behav,2016,141:1-9.
8、 YAN S, YOU ZL, ZHAO QY.Antidepressant-like effects of Sanyuansan in the mouse forced swim test, tail suspension test, and chronic mild stress model[J]. Kaohsiung J Med Sci, 2015;31(12):605-612. YAN S, YOU ZL, ZHAO QY.Antidepressant-like effects of Sanyuansan in the mouse forced swim test, tail suspension test, and chronic mild stress model[J]. Kaohsiung J Med Sci, 2015;31(12):605-612.
9、 POLESZAK E, SZOPA A, WYSKA E.Caffeine augments the antidepressant-like activity of mianserin and agomelatine in forced swim andtail suspension tests in mice[J]. Pharmacol Rep, 2016,68(1):56-61. POLESZAK E, SZOPA A, WYSKA E.Caffeine augments the antidepressant-like activity of mianserin and agomelatine in forced swim andtail suspension tests in mice[J]. Pharmacol Rep, 2016,68(1):56-61.
10、 HAJ-MIRZAIAN A, KORDJAZY N, AMIRI S. Involvement of nitric oxide-cyclic guanosine monophosphate pathway in the antidepressant-like effect of tropisetron and ondansetron in mice forced swimming test and tail suspension test[J]. Eur J Pharmacol, 2016,780(5):71-81. HAJ-MIRZAIAN A, KORDJAZY N, AMIRI S. Involvement of nitric oxide-cyclic guanosine monophosphate pathway in the antidepressant-like effect of tropisetron and ondansetron in mice forced swimming test and tail suspension test[J]. Eur J Pharmacol, 2016,780(5):71-81.
11、 CHELLIAN R, PANDY V, MOHAMED Z. Biphasic Effects of α-Asarone on Immobility in the Tail Suspension Test: Evidence for the Involvement of the Noradrenergic and Serotonergic Systems in Its Antidepressant-Like Activity[J]. Front Pharmacol,2016,7:72. CHELLIAN R, PANDY V, MOHAMED Z. Biphasic Effects of α-Asarone on Immobility in the Tail Suspension Test: Evidence for the Involvement of the Noradrenergic and Serotonergic Systems in Its Antidepressant-Like Activity[J]. Front Pharmacol,2016,7:72.
上一篇
下一篇
出版者信息








《广州医药》公众号
目录