Objective To investigate the frequency of anti-Mur and Mur antigen among blood donors in Huizhou city and provide data for guiding clinical transfusion and establishing red blood cells library of rare blood type. Method Micro-plate brine method and polybrene method are used to screen anti-Mur from 8686 blood donors. DG Gel Coombs cards are used to confirm the result. Human anti-Mur serum is used to screen the phenotypes from other 1119 blood donors. Results The frequency of anti-Mur was 0.35%(31/8686). The differences between men and women had statistical significance (P<0.05), of which women were higher than men. The frequency of Mur antigen positive was 5.61％(63／1119). Conclusion The blood donors in Huizhou showed a high frequency of anti-Mur and a relatively high frequency of Mur antigen, so it is very necessary to add test method of Mur blood group antigen and antibody before the donors carry out the blood transfusion process.