Objective To investigate the clinical distribution and antimicrobial resistance of infections caused by enterobacter cloacae in our hospital. Methods Antimicrobial susceptibility tests were done by Kirby-Bauer disk diffusion method. The extended-spectrum β-Lactamases (ESLBS) were detected by double disc diffusion test. Results A total of 261 strains of E cloacae were isolated from Jan 2010 to Dec 2014. The percentages of these strains isolated in 2010, 2011, 2012, 2013, 2014 were 18.4%, 19.2%, 19.9%, 20.7%, 21.2% respectively. E cloacae were mainly isolated from intensive care unit (ICU) (49.0%), respiratory medicine (14.9%) and surgery (11.9%) respectively. The percentages of these strains isolated from sputum, urine and wound secretion were 52.0% 13.5% and 12.8% respectively. The percentages of ESBLS producer was 29.9% (78 strains).Their resistance was obviously higher than that of non-ESBLS producer. But all strain were sensitive to imipenem. Conclusion E Cloacae caused infections of respiratory tract, urinary and wound principally in clinic. The most of which occured in ICU. The ESBLS are popular in E cloacae and show the increasing trend year by year. The restricted use of antimicrobial drug is a considerable measure which reduces prevalence of ESBLS producing strains.