Objective To explore and analysis the application of leucorrhea smear multi-function BTR staining techniques(method) in gynecological disease. Methods Leucorrhea were taken in 300 gynecological disease patients with obvious infection symptoms, BTR, saline smear, pap staining were performed at the same sample. Results Pathogene positive rate ofBTR method of trichomonad, mould, clue cell, leptotrichia, gonococci, coccobacillus, dyskaryotic cell, cancer cell and vaginal cleanliness detection rate is 42%,36.7%,17.3%,12%,6.7%,86%,6.3%,2.7% and 95% respectively. The pathogens detection rate of BTR was higher than saline smear, pap staining. It showed statistical difference(P<0.01). BTR method operated simply, timely, low cost, positively than saline smear, pap staining,it showed statistical difference(P<0.01). Conclusion BTR method can detect bacterial vaginitis, colpitis mycotica, cervicitis, cervical carcinoma and ovarian cancer. It can provide reliable evidence for gynecological disease diagnosis and treatment. It can be applied in big gynecological and cancer screening. BTR method is deserved to popularize and apply in primary hospital.