目的 探讨男性人乳头瘤病毒（HPV）基因分型感染情况。方法 收集采用聚合酶链式反应反向斑点杂交法进行28种HPV基因分型检测的1 137例男性检查结果,进行回顾性分析。结果 1 137例男性患者中阳性441例,阳性率为38.79%,感染率居前5位的亚型依次为HPV6（11.35%）、HPV11（7.92%）、HPV16（5.10%）、HPV52（3.52%）、HPV43（2.64%）;就诊人群以20~39岁为主,感染人数也最多,各年龄组间阳性率比较差异无统计学意义（P>0.05）,≥50岁组HPV52型阳性率高于20~29岁组（P<0.05）和30~39岁组（P<0.05）。单一感染占67.35%,多重感染占32.65%,单一感染中低危型占比最多（41.27%）,多重感染中,二重感染占比最多（19.50%）,高低危混合感染为各种类型感染之首（15.87%）。结论 1 137例样本中HPV阳性率为38.79%,感染亚型以HPV6、HPV11、HPV16、HPV52、HPV43为主,单一低危型感染较为常见,各年龄组间阳性率相近。

Objective To investigate the genotypes of human papillomavirus（HPV）infection．Methods A total of 1 137 male patients’ diagnoses were collected and analyzed retrospectively,which came from the detections using polymerase chain reaction reverse dot blot hybridization to genotype 28 HPV．Results Among 1 137 male patients,441 were HPV positive,with a positive rate of 38.79%,the infections of top five HPV types were HPV6（11.35%）,HPV11（7.92%）,HPV16（5.10%）,HPV52（3.52%）,HPV43（2.64%）．The majority of the patients were the 20-39 age group,and the number of infections was also the highest．There was no statistical significance on the difference in the positive rate among different age groups（P>0.05）．The positive rate of HPV52 in ≥50 years old group was higher than the groups of aged 20~29（P<0.05）and 30~39（P<0.05）．The single and multiple infections accounted for 67.35% and 32.65%．The low-risk HPV accounted for the highest proportion（41.27%）in single infections,while in patients with multiple infections,the proportion of dual infections was the largest（19.50%）and the high- and low-risk HPV mixed infections was the maximum of the infection types（15.87%）．Conclusions The detection rate of positive HPV in 1 137 male patients was 38.79%,mainly were type 6,type 11,type 16,type 52 and type 43,and the single low-risk HPV infected was common．Positive rates were similar among different age groups．

目的 了解遵义地区住院新生儿TORCH感染状况、发病率,为相关疾病的早期预防和治疗提供重要参考依据。方法 采用回顾性研究方法,对遵义市妇幼保健院2018—2019年住院新生儿弓形虫(TOX)、风疹(RV)、巨细胞(CMV)和单纯疱疹病毒(HSV)Ⅰ型感染结果进行分析。结果 遵义地区4 604例住院新生儿TORCH-IgM的总体阳性率为1.35%,其中CMV、TOX、RV、HSV Ⅰ的阳性率分别是1.00%、0.20%、0.13%和0.02%;对应TORCH-IgG的阳性率分别是67.38%、0.91%,54.54%、22.22%。对2018—2019年住院新生儿TORCH感染筛查结果显示:CMV-IgM阳性率分别从1.47%下降到0.43%(χ²=4.981,P=0.026),RV-IgM的阳性率从0.24%下降到0.00(χ²=12.446,P<0.001),差异均有统计学意义。在研究的2 607例男性患儿中,CMV、TOX、RV、HSV Ⅰ的IgM阳性率率分别是0.19%、0.11%、0.96%和0.04%,其IgG的阳性率为1.07%、55.20%、67.93%和22.48%。在研究的1 997例女性患儿中,CMV、TOX、RV、HSV Ⅰ的IgM阳性率分别是0.20%、0.15%、1.05%和0.00%,其IgG的阳性率为0.70%、53.68%、66.65%和21.88%。不同CMV-IgM感染对谷丙转氨酶(ALT)和谷草转氨酶(AST)的活性影响研究中,CMV-IgM抗体阳性对照组的ALT活性高于阴性组(t=-2.793,P<0.05),AST的活性无差异(t=0.067,P>0.05) 结论 遵义地区4 604例住院新生儿中以CMV感染率相对较高,不容忽视。2018—2019年CMV和RV的新近感染率呈下降趋势。CMV-IgM的感染能引起ALT活性的升高。

Objective To reveal the TORCH infection status and incidence of hospitalized newborns in Zunyi area, and provide important reference for early prevention and treatment of related diseases. Methods The retrospective research method was used to analyze the infection results of toxoplasma gondii (TOX), rubella virus (RV), cytomegalovirus (CMV) and herpes simplx virus (HSV) type I in hospitalized newborns in Zunyi Maternal and Child Health Hospital from 2018 to 2019. Results The overall positive rate of TORCH-IgM in 4 604 hospitalized newborns in Zunyi area was 1.35%, of which the positive rates of CMV, TOX, RV, and HSV Ⅰ were 1.00%, 0.20%, 0.13% and 0.02%, respectively; the corresponding TORCH-IgG positive rates were 67.38%, 0.91%, 54.54%, 22.22%. The TORCH infection screening results of neonates hospitalized in 2018—2019 showed that the CMV-IgM positive rate decreased from 1.47% to 0.43% (χ²=4.981, P=0.026), and the RV-IgM positive rate decreased from 0.24% to 0.00 (χ²=12.446, P< 0.001), the differences were statistically significant. In 2 607 male children, the IgM positive rates of CMV, TOX, RV and HSVⅠwere 0.19%, 0.11%, 0.96% and 0.04%, respectively; and the corresponding IgG positive rates were 1.07%, 55.20%, 67.93% and 22.48%. In 1 997 female children, the IgM positive rates of CMV, TOX, RV and HSVⅠwere 0.20%, 0.15%, 1.05% and 0.00,respectively; and the corresponding IgG positive rate was 0.70%, 53.68%, 66.65% and 21.88%. In the study of effects of different CMV-IgM infection on alanine aminotransferase(ALT) and aspartate aminotransferase(AST) activity, the ALT activity of CMV-IgM positive group was significantly higher than that of negative group (t=-2.793, P<0.05), and there was no difference in AST activity between the two groups (t=0.067, P>0.05). Conclusions The CMV infection rate among 4 604 hospitalized newborns in Zunyi area was relatively high, which should not be ignored. The recent infection rates of CMV and RV showed a downward trend from 2018 to 2019. CMV-IgM infection could cause an increase in ALT activity.