目的 探讨中国人群中不同Rh血型表型的RHAG基因序列。方法 用血清学方法检测20例中国人群中不同Rh血型表型[包括了15例Rh(D)阳性样本和5例Rh(D)阴性样本,Rh(D)阳性样本中包含5例RhCcEe抗原弱表达、5例弱D和5例正常Rh(D)阳性样本],依据RHAG基因序列的特异性,设计10对特异性引物扩增20例样本的RHAG基因第1~10外显子,确认条带符合后送公司纯化并测序。结果 20例不同Rh血型表型的样本在统一PCR条件下均扩出RHAG基因1～10外显子,扩增产物测序结果与标准序列对比一致。结论 了解中国人群中RHAG基因的多态性需观察更多有意义的样本。

Objective To explore the RHAG gene sequence of different Rh phenotypes in Chinese population. Methods We detected different Rh phenotypes in 20 cases of Chinese population using serological tests including 15 cases of Rh (D) positive samples and 5 cases of Rh (D) negative samples. Rh (D) positive samples included 5 cases of RhCcEe antigen weak expression, 5 cases of weak D and 5 cases of normal Rh (D) positive samples. According to specific RHAG gene sequence, 10 pairs of specific primers were designed to amplify 10 exon sequences of RHAG gene. Amplification products confirmed by electrophoresis, then the PCR products were purified and sequencing in a company. Results The exon 1~10 of RHAG gene in 20 cases of Chinese population coding region was sequenced, and the results were consistent with the sequence of GenBank standard. Conclusion More significant samples should be observed to study polymorphism of RHAG gene in Chinese population.