目的 探讨低机械指数诊断超声及低声压治疗超声对造影剂微泡的作用效果。方法 低机械指数的诊断超声及不同声压下低能量治疗超声体外辐照造影剂微泡,通过获得的超声造影图像间接分析微泡总浓度的变化,探讨不同机械指数诊断超声及不同声压治疗超声下微泡发生稳定空化及惯性空化情况。结果 低机械指数(<0.2)诊断超声及低声压(<0.15 MPa)治疗超声下微泡以稳定空化为主,随着机械指数增高或声压增高,微泡以稳定空化和惯性空化两种方式并存,当机械指数>0.3或声压>0.2 Mpa时,微泡以惯性空化为主。结论 微泡在低机械指数及低声压作用下发生不同空化效应,随着机械指数或声压增加,微泡破坏增加,以惯性空化效应为主。

Objective To investigate the effect of low mechanical index diagnostic ultrasound and low acoustic pressure therapy on contrast agent microbubbles. Methods Microbubbles were irradiated by diagnostic ultrasound with low mechanical index(MI) and low energy therapy under different acoustic pressure. To obtain the images, and analyze the change of microbubble concentration,the movement trend of microbubbles under different MI and different acoustic pressure were analyzed, to estimated stable cavitation or inertial cavitation of microbubbles. Results Stable cavitation was the main form of microbubbles under low mechanical index (< 0.2) diagnostic ultrasound and low acoustic pressure (< 0.15Mpa). With the increase of mechanical index or acoustic pressure, stable cavitation and inertial cavitation coexisted. When mechanical index > 0.3 or sound pressure > 0.2MPa, microbubbles were mainly inertial cavitation. Conclusion The cavitation effects of microbubbles under low mechanical index and low acoustic pressure are different. With the increase of mechanical index or acoustic pressure, the damage of microbubbles increases, and main effect is the inertial cavitation.

目的 探讨微泡增强的超声空化对荷瘤兔乳腺癌的治疗效果。方法 建立兔VX2乳腺癌模型,肿瘤兔随机分成两组,超声微泡组及单纯超声组,各组于治疗前及治疗后分别行超声造影,分析造影前后肿瘤灌注情况。结果 超声微泡组治疗前后造影灰阶值改变明显,造影灰阶值从治疗前的(20.26±2.59)降至(3.71±1.61)(P<0.01);而单纯超声组肿瘤造影灰阶值无明显统计学差异(P>0.05)。结论 微泡增强的超声空化对荷瘤兔乳腺癌有一定的治疗效果。

Objective To investigate the treatment effect of microbubbles-enhanced ultrasound cavitation on VX2 breast tumor in rabbits. Methods The rabbit model of VX2 breast tumor were established. The rabbits were randomly divided into two groups, microbubbles-enhanced ultrasound (US+MBs) group and standard ultrasound (US) group. The VX2 breast tumor perfusion were imaged and assessed using contrast enhanced ultrasonography (CUES) before and after treatment. Results The contrast enhanced grayscale value (GSV) of VX2 breast tumors decreased significantly in the US+MBs group, from 20.26±0.59(before treatment) to 3.71±.761 (after treatment)(P<0.01). While there was no significance of GSV before and after treatment (P>0.05) in the US group. Conclusion Microbubble-enhancement ultrasound cavitation has a certain therapeutic effect on the VX2 breast tumor in rabbits.

目的 探讨微泡增强的超声空化增加睾丸组织的药物浓度的可行性。方法 18只雄性8月龄性成熟新西兰兔随机分为空白对照组(C)、单纯微泡组(MB)、治疗超声组(TUS)、超声联合微泡辐照组(MEUS)4组,每组各9个。MB组给予静注微泡造影剂 0.1 mL/kg ;TUS组给予超声辐照5min;MEUS组给予静注微泡造影剂0.1 mL/kg的同时超声辐照5min;每组在治疗前5min均经耳缘静脉注射2%伊文思蓝(EB)2.5 mL/kg;治疗后1 h取各组睾丸组织制备组织匀浆测量 EB 浓度。结果 MEUS组兔睾丸组织内 EB 浓度高于其他各组(P<0.05),差异有统计学意义。结论 微泡增强的超声空化可以明显提高睾丸组织内EB浓度。

Objective To investigate feasibility of microbubbles enhanced ultrasound (MEUS) on the concentration of Evans blue (EB) in rabbit,stestis. Methods Eighteen sexually mature male New Zealand rabbits were divided into four groups randomly. Pulsed ultrasound irradiation and intravenous microbubbles injection were both applied in the microbubbles enhanced ultrasound group (MEUS), pulsed ultrasound irradiation and intravenous microbubbles injection were individually applied in the therapeutic ultrasound group (TUS) and the simple microbubbles group (MB). Injection of EB was applied in each group five minutes before the treatment.Concentration of EB in testis tissue was measured homogenated. Results The concentration of Evans blue(EB) in rabbit's testis in MEUS group was statistically higher than the other groups (P<0.05). Conclusion Microbubbles enhanced ultrasound (MEUS) can increase the concentration of Evans blue (EB) in rabbit's testis.