目的 筛选九头狮子草治疗小鼠细菌性阴道炎的有效部位。方法 采用金黄色葡萄球菌、大肠埃希菌菌悬液注入小鼠阴道建立小鼠细菌性阴道炎动物模型,观察九头狮子草各极性部位对细菌性阴道炎的治疗作用。结果 九头狮子草水提液、石油醚提取部位和70%乙醇提取部位对病原菌转阴率和阴道黏膜炎症的治愈程度均有提高,其中,对金黄色葡萄球菌的转阴率分别为60%、50%、40%,对大肠埃希菌的转阴率分别为80%、70%、50%。结论 九头狮子草石油醚提取部位和70%乙醇提取部位为其治疗细菌性阴道炎的有效部位。

Objective To study the active parts of Peristrophe japonica of vaginitis treatment effect. Methods The bacterial vaginitis model was established by injecting the bacteria of Staphylococcus aureus and Escherichia coli into the vagina of the mice and the treatment effect of each extract of Peristrophe japonica was observed. Results Three drug administration groups (including water extract of Peristrophe japonica, ligarine fraction and 70% ethanol fraction) showed marked treatment effect on the vaginitis models. The effect of water extract of Peristrophe japonica group were stronger than that of the ligarine fraction and 70% ethanol fraction groups. The pathogens seroconversion rates in Staphylococcus aureus were orderly as follows: 60%, 50%, 40%. The pathogens seroconversion rates in Escherichia coli were orderly as follows: 80%, 70%, 50%. Conclusion The ligarine fraction and 70% ethanol fraction of Peristrophe japonica might be the active extracts of curing vaginitis.

目的 研究细胞周期调控因子E2F-1和p16蛋白与宫颈上皮内瘤变及宫颈鳞癌的表达及其临床意义。方法 通过免疫组化SP法检测宫颈上皮内瘤变Ⅰ-Ⅱ级(CINⅠ-Ⅱ级)、宫颈上皮内瘤变Ⅲ级(CINⅢ级)、宫颈鳞癌各40例中E2F-1和p16蛋白的表达情况,并用20例慢性宫颈炎组织作对照。结果 E2F-1蛋白在CINⅠ-Ⅱ级、CINⅢ级和宫颈鳞癌组中阳性率分别为17.5%、67.5%和80.0%, 3组间有差异(χ²=37.278,P<0.001)。p16蛋白表达阳性率分别为27.5%、70.0%和82.5%,3组间差异有统计学意义(χ²=27.708, P<0.001)。CINⅠ-Ⅱ级与CINⅢ级组及宫颈鳞癌组比较均有差异,但CINⅢ级与宫颈鳞癌组间无差异。p16蛋白的表达与E2F-1蛋白表达有正相关关系;在鳞癌组中E2F-1与p16的异常表达与组织学分级、临床分期有关(P<0.05)。结论 子宫颈鳞癌的形成与E2F-1 、p16蛋白过表达是呈正相关关系,E2F-1 、p16 蛋白可能作为子宫颈鳞癌及CIN的标志物,对子宫颈癌筛查和预防有重要意义。

Objective To explore the clinicopathologic significance of the protein expression of cell cycle regulators E2F-1 and P16 in cervical intraepithelial neoplasia(CIN) and cervical squamous cell carcinoma(SCC). Methods Immunohistochemical technique S-P was used to determinate the expression of E2F-1 and P16 protein in 40 cases with CINⅠ-Ⅱ, 40 cases with CINⅢ and 40 cases with cervical squamous cell carcinoma. Results The positive rate of E2F-1 protein expression in CINⅠ-Ⅱ, CINⅢ and cervical squamous cell carcinoma was 17.5%,67.5% and 80.0%, respectively. There were significant differences among the three groups(χ²=37.278,P<0.001). The positive rate of P16 protein expression in CINⅠ-Ⅱ, CINⅢ and cervical squamous cell carcinoma was 27.5%,70.0% and 82.5%, respectively. There were significant differences among the three groups (χ²=27.708, P<0.001). The group with CINⅢ and cervical squamous cell carcinom compared with the group withCINⅠ-Ⅱ did have differences. But there were no significant differences in the group with CINⅢ and the group with cervical squamous cell carcinoma. The relative expressions between P16 and E2F-1 showed positive correlation. In the cervical squamous cell carcinoma group, the abnormal expression of E2F-1 and p16 were correlated with the histological grade and clinical stage. Conclusion It showed positive correlation between very high expression of p16 protein and E2F-1 infection in CIN and SCC. p16 protein and E2F-1 can be an indicator in SCC and CIN,which has very significance in preventing and screening of cervical cancer.