Objective To analyze the characteristics,occupational exposure links, types, and treatment methods of occupational exposure among healthcare staff hospital, providing a theoretical basis for the development of related intervention strategies for hospitals.Methods A retrospective study was conducted to explore 301 cases of occupational exposure among healthcare staff in The People’s Hospital of Yuxi City from 2018 to 2023.Data of 301 cases were collected, including information on gender, age,department,personnel category, length of service in the hospital, job title, and the circumstances of occupational exposures, which covered exposure links, type, source, and post-exposure medication treatment measures.Results Among the 301 healthcare staff experiencing occupational exposure, the average age was(28.81±10.92)years, with 84.7%(255 cases)being female.Occupational exposure most frequently occurred during procedures such as examination, puncture, injection, blood collection, catheterization, treatment, and surgery(57.8%,174 cases), followed by the disposal of medical waste(38.9%, 117 cases).The primary type of exposure was needle-stick injury(87.7%).Contact with patients suffering from blood-borne infectious diseases accounted for 45.5%(137 cases)of the occupational exposure incidents.There were significant differences in exposure types, job positions, departments, sources of exposure, and post-exposure medication treatment measures among healthcare staff of different categories(P<0.05).Conclusions To prevent the occurrence of occupational exposure among healthcare staff, it is necessary to enhance training on occupational exposure knowledge and emergency management, particularly improving the disposal of medical waste and clinical operational skills.Additionally, it is crucial to have personalized training programs tailored to healthcare staff based on their varying lengths of service and positions.