肝胆胰外科中吲哚菁绿荧光成像技术的应用研究进展

来源期刊：广州医药 | 133-143 发布时间：2026-02-20 收稿时间：2026/4/8 16:40:48 阅读量：115

作者：: 黄宇,

匡姣,

刘辉,

伍兆锋,

翁杰锋,

张帅,

古维立,

关键词：: 吲哚菁绿荧光导航技术腹腔镜手术微创外科肝胆胰外科; indocyanine green fluorescent navigation technology laparoscopic surgery minimally invasive surgery hepatopancreaticobiliary surgery

DOI：: 10.20223/j.cnki.1000-8535.2026.02.002

收稿时间：: 2025-03-11
修订日期：
接收日期：
引用总数：: 1

吲哚菁绿（ICG）荧光成像技术基于ICG独特的代谢特性及近红外光激发特性, 通过动态荧光显影成像为手术提供精准导航, 其在肝胆胰外科领域展现出重要的应用价值。随着多版国内外指南的迭代更新, ICG荧光导航技术已被确立为肝胆胰外科手术的重要辅助手段, 其安全性和有效性得到充分验证。文章就ICG荧光成像技术的原理、在肝胆胰外科应用中的现状、在临床应用中面临的问题以及其应用展望展开综述。

古维立 日本长崎大学医学博士，主任医师，博士生导师，日本长崎大学客座教授，现任广州医科大学附属中医医院肝胆外科主任导师。历任广州市第一人民医院大外科主任、普外科主任、肝胆胰外科主任。现为广州市医学会加速康复外科分会主任委员、广东省医学会加速康复外科分会副主任委员、广东省医师协会肝胆分会ERAS组组长、广东省医师协会肝胆分会胆道学组副组长、广东省医师协会普通外科分会副主任委员、日本肝胆胰外科学会委员。2015年获首届“羊城好医生”称号。

主要研究方向：肝癌肿瘤干细胞构建肝癌类器官研究，肝纤维化再生机制与小分子药物影响的研究，肝脏移植免疫耐受机制的研究等。主持多项省项部级课题，获得国家发明及实用新型技术专利各1项。近三年以第一或通讯作者发表SCI论文15篇，累计影响因子60.9分。

吲哚菁绿（indocyanine green，ICG）荧光成像技术又称为ICG标记的近红外荧光辅助下的荧光成像技术。ICG作为新型分子显影剂，凭借其独特的代谢特性在肝胆胰外科领域展现出重要价值。ICG经静脉注射后迅速与血浆蛋白结合，通过肝细胞特异性摄取，经胆汁排泄的独特代谢路径（不参与肠肝循环及肾代谢），为临床应用奠定了生物学基础。借助近红外光激发特性（激发波长750~810 nm，发射波长840 nm），ICG可在穿透8~10 mm组织后形成可视化荧光图像，突破了传统显影技术的深度限制。随着多版国内外指南的迭代更新^［1-10］，ICG荧光导航技术已被确立为肝胆胰外科手术的重要辅助手段，其安全性和有效性已得到充分验证。笔者就ICG荧光成像技术的原理、在肝胆胰外科应用中的现状、在临床应用中面临的问题以及其应用展望展开综述如下。

1 ICG 荧光成像的原理

ICG荧光成像技术在临床的广泛应用得益于其体内特殊的药代动力学改变。ICG在体内的代谢排泄过程如图1。ICG经静脉注射后即刻与血浆蛋白紧密结合，不易分解，也不改变蛋白的分子结构，色素不沉着于皮肤，也不被其他组织吸收，其随血液循环迅速分布于全身血管内，高选择性且高效率地被肝细胞摄取，迅速地以游离形式排泄到胆汁中，不参与体内其他代谢反应；随后其随胆汁经肝内外胆道系统排泄至十二指肠，不参与肠肝循环，也无淋巴回流，不被肝脏再次摄取，也不从肾脏排泄，最终随粪便排出体外^［11］。

图 1 ICG 体内代谢示意图与临床应用

注：经外周静脉注入的ICG高效率地被肝细胞摄取，迅速地以游离形式排泄到胆汁，后随胆汁经肝内外胆道系统排泄至十二指肠，不

参与肠肝循环，最终随粪便排出体外。ICG在近红外光照射下可显示绿色荧光，ICG滞留在肝组织可显影肝脏，滞留在胆管可显影胆管，滞留肠道可显示肠管，从吻合口处漏出腹腔可判断胆漏和肠吻合口瘘。

ICG在波长为750~810 nm的近红外光照射下能够发射波长约840 nm的近红外光，该波长光能穿透8~10 mm厚度的组织和器官，在被荧光摄像机捕获后经光电转换形成肉眼可见的绿色荧光图像。研究显示，正常情况下ICG代谢迅速，在给药后30 s即出现于肝动脉系统，40 s后可在门静脉中，40～50 s ICG开始从肝窦向肝细胞的运输，从而随肝脏的血液部分流出，15 min内完成90%的体循环清除，20~30 min实现97%的胆道排泄，这种动态代谢特征为手术导航提供了精准时间窗。

简而言之，肝内外胆道的荧光显影是由于ICG排泄至肝内外胆管而显影；原发性肝癌肝肿瘤的荧光显影则是由于肿瘤组织无法像正常肝组织一样经胆道排泄，造成ICG 滞留而显影；肝外的肝转移灶仍具有摄取ICG的能力，由于排泄障碍，ICG滞留显示荧光^［12］；对于结直肠癌肝转移者，病灶本身不摄取ICG，但由于压迫周边肝脏胆管引起ICG滞留而显影^［13］，表现为边缘环形染色。此外，肝癌的门静脉癌栓、胆管癌栓也可摄取ICG排泄障碍而显示荧光^［14］。

2 肝脏外科中 ICG 荧光成像的应用

2.1 肝肿瘤的可视化

ICG在术中高灵敏度识别肝细胞癌（Hepatocellular carcinoma，HCC）及转移性肝癌，做到肝肿瘤的可视化（图2A）。原发性肝癌因ICG滞留形成均匀的“癌型荧光”，而转移性肝癌则表现为“边缘型荧光”^［15-16］。2009年Ishizawa等^［13］首次系统评估了ICG荧光成像在肝癌术中的实时检测价值。研究纳入37例HCC患者和12例结直肠癌肝转移患者，术前静脉注射ICG后通过近红外摄像系统分析病灶，结果显示ICG荧光成像成功识别全部病理证实的63个HCC病灶和28个转移灶，其中包括8个肉眼不可见但荧光显影的HCC（含5例早期癌）病灶。进一步的分析发现，分化良好的HCC呈现均匀强荧光，转移灶则表现为边缘荧光，证实了该技术对肝肿瘤的精准可视化能力。

图 2 ICG 显影不同部位的图片

注：（A）ICG荧光显影肝细胞癌；（B）ICG荧光显影肝血管平滑肌脂肪瘤；（C）ICG荧光区分肝囊肿边界；（D）ICG荧光显影肝内

胆管；（E）ICG荧光显影肝外胆管。

2013年Satou等^［12］进一步探索了ICG荧光成像对HCC肝外转移灶的术中检测效能，实现了肝外转移灶的可视化突破。研究纳入17例疑似肝外转移（肺、肾上腺、淋巴结、腹膜等部位）患者，术中使用近红外荧光系统检测，结果显示28个荧光显影病灶中24个为HCC转移（含5例新检出病灶），而33个切除病灶中26个荧光阳性者均为转移灶，7个荧光阴性病灶中仅1例为漏检转移。活体及离体检测的阳性预测值均达100%，提示该技术对隐匿性肝外转移灶具有高特异性，但需结合其他手段降低漏诊风险。对于亚厘米级肝癌^［17］，ICG荧光成像也能显著提升其术中检出率。Ishizawa等^［13］于2009年首次实现ICG引导下微小肝癌（直径≤10 mm）的实时识别。Handgraaf等^［18］的多中心研究表明，结直肠癌肝转移切除术中，ICG荧光成像的新病灶检出率（25%）高于传统方法（13%），且新检出的病灶平均直径仅3.2 mm，而传统方法检出的病灶平均直径为7.4 mm。Peloso等^［19］进一步证实，ICG联合术中超声对直径≤3 mm转移灶的检出率优于术前CT或单独超声，但对直径＞3 mm病灶的灵敏度差异无统计学意义。可见ICG联合超声对隐匿性肝外转移和亚厘米病灶的独特优势，该项技术实现了肝癌及转移灶的术中高可视化检出率。

2.2 肝段边界的标记

ICG荧光成像技术通过门静脉流域特异性染色，为解剖性肝切除术中肝段边界的精准标记提供了重要手段。自2008年将ICG荧光成像用于肝段/亚段识别以来，该技术不断优化并拓展至腹腔镜及复杂肝段切除领域。2008年Aoki等^［20］开创性应用ICG荧光成像标记肝段边界，为解剖性肝切除术提供实时导航支持。2016年Ishizawa等^［21］提出，门静脉注射ICG或结合门脉阻断后静脉注射可清晰标记目标肝段，为精准切除奠定技术基础。2017年王宏光等^［22］完成国内首例ICG荧光引导下腹腔镜解剖性肝段切除术，验证了ICG荧光融合影像在术中导航的应用价值；同年，Kobayashi等^［23］系统回顾了东京大学医院105例患者的临床数据，对比了多种荧光染色技术的术中效果。结果显示，所有技术均能有效显影门静脉流域边界，且无ICG相关不良事件发生。术后严重并发症（ClavienDindo≥Ⅲ级）发生率仅为7.6%，证实了技术的安全性。2018年Meng等^［24］采用ICG荧光腹腔镜导航设备完成国内首例荧光导航下解剖性右肝切除，进一步推动该技术的临床普及。近期，Hu等^［25］通过系统综述与meta分析评估ICG荧光导航在HCC肝切除术中的效果。结果显示，相较于传统手术，ICG组显著缩短手术时间、减少术中出血及输血率，并提高R0切除率及降低并发症发生率，进一步说明了ICG荧光导航在HCC治疗中的有效性和安全性，建议推广应用。

近年来，ICG荧光导航技术也逐步应用于机器人肝切除术，通过多维功能协同，显著提升了机器人肝切除术的精准性和安全性，成为深部肿瘤定位、切缘评估及隐匿病灶识别的关键工具。Kinoshita等^［26］聚焦于ICG荧光成像在机器人辅助肝切除术中的肿瘤定位价值，结果显示84%（26/31）的肿瘤在肝表面被荧光识别，术中有8例通过实时荧光信号调整切缘；ICG组患者肝切缘阴性率达100%（仅1例胆管切缘阳性），而未使用ICG的2例中1例切缘阳性，证实其显著提升手术根治效果。Rompianesi等^［27］系统评估了ICG荧光技术在76例机器人肝切除术中的个体化应用价值。结果显示ICG显影成功识别94.9%的目标肿瘤，并额外检出3例隐匿病灶；33.9%的病例通过荧光实时评估切缘，32.9%的病例评估残肝灌注，7.9%的病例据此调整切除范围，同时25%的病例采用负染色标记肝段边界，血管及胆管可视化率分别达21.1%和9.2%。研究全程无ICG相关不良反应，且51.3%为高难度手术，证实了该技术在复杂手术场景中的可靠性。Kim等^［28］报道了4例通过机器人辅助结合ICG荧光成像技术实施左肝切除术，采用Glissonean外途径联合间段平面显影策略。4例患者均实现精准肝实质切割，无中转开腹和并发症，切缘均为阴性。该技术利用肝脏无Glissonean分支的间段平面解剖特点，借助机器人精细操作和ICG导航，提升了解剖性肝切除的微创性与安全性，为复杂肝胆手术提供了新的技术范式。由此可见，ICG荧光导航通过肿瘤显影、切缘评估、解剖标记等多功能整合，为机器人肝切除术提供了智能化解决方案，尤其在深部肿瘤和隐匿病灶处理中优势明显。以上研究共同证实，ICG荧光成像技术亦可提升机器人手术的根治性并降低并发症风险。

2.3 肝癌的光动力治疗

基于ICG在HCC中的特异性蓄积特性，其可作为光热剂、光敏剂及荧光探针，通过靶向聚集于肝癌细胞实现光动力治疗，为肝癌精准诊断和治疗提供新方向^［29-31］。Shirata等^［30］通过Huh7肝癌裸鼠移植瘤模型验证了ICG光动力治疗的疗效。结果表明注射ICG 24 h后，采用823 nm近红外激光（第1、7天）照射肿瘤，3 min内局部温度升至48.5 ℃，并通过产生活性氧诱导细胞凋亡；重复照射后，第9天肿瘤体积缩小至87 mm³ （对照组1 332 mm³ ，P=0.01）。研究揭示了ICG光热动力疗法的抗肿瘤作用依赖光热效应与氧化应激的双重机制，且重复治疗可协同增效。进一步基础研究表明，ICG在肝癌细胞中的特异性蓄积与脂质代谢异常、膜转运蛋白调控等机制相关，为靶向光动力治疗提供了分子生物学依据。这些成果标志着ICG从传统肝功能检测工具向肝癌诊疗一体化核心角色的跨越式转变，其多模态治疗潜力亟待临床转化探索。

2.4 辅助肝囊肿开窗

ICG荧光成像也可安全应用于肝脏良性疾病中（图2B-C），在肝血管平滑肌脂肪瘤中辨别边界，在肝囊肿开窗中可以帮助术者清晰区分囊肿壁、周围肝组织及潜在胆管结构^［32］。相较于传统白光腹腔镜，ICG荧光显影具有高对比性，囊肿壁与正常肝组织的荧光差异可辅助界定囊肿边界，避免过度切除或遗漏病灶，可降低术中出血和胆漏风险^［33］。ICG荧光胆管造影可清晰地显示肝内胆管结构（图2D），尤其在处理巨大肝囊肿时，能有效识别囊肿内壁或邻近的隐匿胆管分支，从而降低术中胆管损伤风险^［34-35］。研究显示，ICG引导下腹腔镜肝囊肿开窗术术后3个月无胆管损伤或胆漏等并发症^［34］。笔者研究团队也报道了1例ICG荧光辅助下同时行胆囊切除及肝囊肿开窗的病例^［33］，术中清晰地识别了肝内外胆管以及肝囊肿壁与肝组织的边界，避免了胆道损伤，尤其适用于解剖结构复杂者。

3 胆道外科中 ICG 荧光成像的应用

3.1 术中胆道显影

ICG荧光成像技术自引入腹腔镜胆囊切除术（laparoscopic cholecystectomy，LC）后，逐步成为胆道外科精准化手术的重要工具。其核心优势在于利用ICG的肝胆排泄特性，通过近红外荧光显影实现胆道结构的实时可视化（图2E），显著降低术中误损伤风险，提高LC手术的安全性^［36］。Ishizawa等^［37］开创了ICG胆道造影的先河，证实其可清晰地显示胆囊三角的副胆管及解剖变异结构，特异度与灵敏度俱佳。后续研究进一步拓展了其应用场景，Jin等^［38］比较了传统LC和ICG-LC对胆管损伤的应用价值，结果表明ICG荧光成像辅助LC能清晰地显示胆道结构，较传统术式降低胆管损伤并发症发生率（1.8% vs 11.1%），临床应用价值高。van den Bos团队^［39］的研究表明，ICG荧光导航使术者更快识别胆囊管并达成安全视野，提升了手术效率。Castagneto-Gissey等^［40］研究了经胆囊途径注射ICG的优势，结果表明与静脉注射ICG 相比，胆囊内 ICG 途径可以通过避免肝脏背景荧光，提供更好的信噪比，从而提高胆管与肝脏的对比。Zhou等^［41］在胆总管切开取石术中发现，ICG组胆道显影成功率达100%，显著缩短切口定位时间并减少术中出血；针对复杂病例，Huang等^［42］在38例困难胆囊手术中证实ICG显影可突破严重粘连限制，减少出血量并避免中转开腹，凸显其在复杂解剖中的价值。陈志等^［43］研究了经胆囊注射ICG在腹腔镜困难型胆囊术中的应用，结论表明困难型胆囊患者LC术中经胆囊注射ICG显影简单易行，可在困难型胆囊术中显示胆囊及胆管结构，有助于安全高效解剖肝外胆管及胆囊床的同时加快手术进度，减少手术创伤。Zhang等^［44］的报告进一步概述了在急性坏疽性胆囊炎所致的困难LC术中进行ICG荧光导航的主要步骤，ICG荧光成像效果满意，安全有效。近期，Jayakumar等^［45］探讨了ICG荧光导航手术在儿童肝胆手术中的可行性。通过前瞻性观察17例患儿（胆道闭锁9例、胆总管囊肿6例、胆囊结石2例），术前按0.5 mg/kg标准剂量静脉注射ICG，术中实时显影观察解剖结构。结果显示ICG在3种手术中均清晰显示胆道走行（如闭锁术中肝内纤维束荧光梯度变化），协助安全分离并避免损伤，且未发生ICG相关不良反应。

总而言之，以上研究证实该技术安全有效，既可辅助复杂胆道手术安全实施，也可辅助儿童肝胆手术的精准操作，未来需通过标准化方案和量化分析进一步优化临床应用。针对ICG给药时机与剂量标准化问题，笔者团队通过系列研究提出优化方案，如术前10~12 h静脉注射10 mg ICG可实现稳定的显影^［46］；针对急诊或限期手术，术前30 min注射0.1 mg（高清荧光系统）^［47］或10~25 μg ICG（结合4K荧光系统）^［48］即可清晰显示肝外胆管，为临床提供灵活选择。笔者团队的经验与国内其他研究团队的临床使用剂量经验一致^［49］，值得在基层医院及初学者中推广应^用［50］。

3.2 胆漏和吻合口瘘的识别

ICG荧光成像技术凭借其高灵敏性、无创性和实时可视化优势，在胆漏及吻合口瘘的术中识别与术后诊断中也展现出重要临床价值。其通过动态示踪胆道渗漏或吻合口缺损，为外科医生提供了精准的术中导航及术后早期预警工具。笔者研究团队近期报道了ICG荧光显影在术中发现微小胆漏的典型案例^［51］。术中静脉注射ICG后，其经胆道排泄的特性不仅可清晰勾勒胆管解剖结构（降低误损伤风险），而且能实时捕捉胆汁渗漏点。荧光显影下，微小胆漏表现为局部异常荧光聚集，术者可即刻修补缺陷，从而显著降低术后胆漏发生率。这一技术的应用突破了传统依赖术者经验或被动观察的局限，实现了胆漏的主动防控^［52-53］。

为进一步拓展ICG的应用场景，笔者团队还创新性建立了基于腹腔引流液ICG荧光检测术后吻合口瘘的早期诊断方法^［54］。研究首先在大鼠结肠吻合口瘘模型中验证可行性，术后静脉注射ICG后24 h，吻合口瘘组腹腔引流液即检测到特异性绿色荧光，48 h荧光信号进一步增强，证实ICG可通过吻合口瘘部位富集于腹腔。随后，团队将这一发现转化至临床，对接受腹腔镜直肠癌手术的患者经外周注射ICG后，收集注射ICG后8~12 h的腹腔引流液，所有临床确诊吻合口瘘病例及16.7%的高危吻合口瘘临床病例的引流液均检测到显著荧光信号，其检测灵敏度为100%，特异度达83.3%。表明ICG荧光检测不仅能明确诊断吻合口瘘，还可识别潜在风险病例，为早期干预赢得时间窗。

3.3 胆管灌注评估

近期，Lee等^［55］探讨了ICG荧光成像在活体肝移植中评估胆管灌注与术后胆道并发症的关系。对9例患者术前行ICG预处理，实时监测胆管荧光的最大值、半衰期、上升斜率等参数。结果显示2例发生术后胆道并发症的患者胆管荧光半衰期显著延长且斜率降低，提示胆管灌注异常可能与术后并发症相关。研究初步表明，ICG荧光成像可通过量化胆管血流动力学参数，为预测术后胆道并发症提供潜在影像学依据，但需更大样本量的研究验证其临床适用性。

4 胰腺外科中 ICG 荧光成像的应用

4.1 精准肿瘤切除与边界界定

在胰腺肿瘤外科中，ICG荧光显影技术通过靶向标记肿瘤边界与异常血管，显著提升手术的根治性与安全性。曾宁团队率先采用术中局部注射ICG技术，成功实现胰腺神经内分泌肿瘤的边界可视化，为完整切除提供实时导航^［56］。方驰华和杨剑团队进一步优化给药策略，结合动态灌注与三维标记染色技术，提升了胰岛素瘤的荧光对比度，所有切缘均达病理阴性^［57］。Li等^［58］则通过对比近红外一区与二区成像窗口，提出红外二区适用于术中实时高穿透显影，而红外一区在长时间监测中因肿瘤血管渗漏滞留效应更具优势，为不同术式需求提供选择依据。Zhou等^［59］开发了一种靶向胰腺导管腺癌的新型近红外荧光探针，通过ICG与癌细胞表面过表达的巢蛋白结合，显著提高了肿瘤信号背景比。在原位和皮下胰腺导管腺癌小鼠模型中，ICG与巢蛋白的靶向结合实现了精准的肿瘤边界显影，指导荧光引导下肿瘤切除并提升效率；进一步结合光热消融技术，可实时识别转移灶并进行干预，为胰腺导管腺癌的精准手术及治疗提供了新策略。

4.2 胰腺灌注评估与胰瘘风险预测

ICG动态显影技术通过量化微循环灌注差异，为胰腺残端血流评估及术后胰瘘防控提供客观指标。Reed等^［60］发现，胰腺肿瘤区域荧光强度斜率与血管通透性显著相关，提示ICG动力学可反映胰腺组织灌注功能。Ghimire团队^［61］在胰十二指肠切除术中应用ICG评估残端血流，使89.2%病例实现灌注优化，术后胰瘘发生率降至7.14%。Chen等^［62］的FLUOPAN研究进一步证实，机器人手术中ICG识别缝合后低灌注区域可预警胰瘘风险，指导术中即时修正。然而，Robertson等^［63］的系统综述指出，当前证据尚无法确立灌注不足与胰瘘的因果关系，亟须标准化评估体系及多中心验证。

4.3 复杂术式中的功能保留与安全性提升

在保留器官功能的创新术式中，ICG技术通过精准显影关键结构降低手术风险。Huang等^［64］在保留十二指肠的胰头切除术中应用ICG，使胆管损伤率从60%降至10%。Lu团队通过优化ICG给药时机，将胆管识别率提升至93.3%，并实现术后零胆漏^［65］。Wang等^［66］在急诊中央胰腺切除术中，利用ICG识别缺血区域，成功保留正常胰腺组织并避免全胰切除。尽管上述研究显示ICG在功能保留中的潜力，但高胰瘘率提示需进一步整合吻合技术创新与ICG动态监测。

5 临床应用挑战与展望

作为新兴的术中导航技术，ICG荧光成像在肝胆胰外科中展现出显著优势，但其临床应用仍面临多重技术瓶颈与标准化挑战。第一，技术参数标准化困境。ICG荧光显影效果受剂量、时机、设备等多因素影响，目前尚未形成统一的操作规范，ICG 给药的时间点和剂量仍需要在日常临床实践中根据具体情况进行调整^［67］。临床研究表明，不同中心对ICG注射剂量（0.1~25 mg）与给药时机（术前30 min至7 d）的选择存在显著差异，其根源在于设备灵敏度差异及组织厚度限制（仅8~10 mm有效穿透）。Kose等^［68］发现ICG对深部病灶（深度＞10 mm）的识别率骤降至4%，需联合术中超声（识别率94%）弥补空间分辨率不。此外，设备厂商的算法差异导致荧光强度量化缺乏可比性，阻碍了多中心研究的横向整合。其二，诊断特异性与生物学干扰。在肝硬化等病理状态下ICG的非特异性分布特性易引发假阳性问题。Ishizawa团队^［13］报道HCC术中ICG显影假阳性率达10.5%，肝硬化患者更升至40%，主要源于再生结节与胆管增生的荧光干扰^［67］。Masuda等^［69］进一步指出，ICG在失代偿期肝硬化中的特异性较CT降低23%，需结合表观扩散系数或靶向分子探针提升鉴别能力。肝脏功能异质性亦导致ICG显影的不稳定性，梁霄等^［70］提出，基于ICG 15 min滞留率（ICG retention rate at 15 min，ICGR15）调整给药时间（ICGR15≤7%者间隔≥48 h，ICGR15＞7%者间隔＞6 d），但该策略对急性炎症或胆道梗阻患者的适用性仍待验证。第三，临床应用可及性与技术转化壁垒。尽管ICG荧光系统已纳入多版指南推荐，其推广仍受制于硬件成本与操作门槛。基层医院因设备昂贵及技术培训缺失，普及率仍不高。将来国产化设备研发将使成本降低，模块化设计将加速基层普及。

ICG荧光成像的应用前景巨大，正逐步从术中解剖导航向多功能整合的精准诊疗体系迈进。技术革新方面，近红外二区荧光成像凭借更深的组织穿透（穿透深度2~3 cm）和更高分辨率，显著提升深部微小肿瘤（如肝切缘残留）的检出灵敏度^［71-72］，而靶向探针结合荧光断层扫描可实现肿瘤微环境的三维可视化。近期，Lin等^［73］开发了一种针对肝癌成纤维细胞激活蛋白（fibroblast activation protein，FAP）的新型近红外一区/二区荧光成像探针。通过合成含反FAP肽的ICG衍生物，该探针可特异性靶向FAP，在体内外研究中实现了对肝癌模型的精准显影，为肝癌的早期检测、微环境解析及转移风险评估提供了可视化新工具。

多模态整合中，人工智能通过量化荧光信号、自动识别胆道变异及预测并发症风险，推动术中决策智能化；联合超声弹性成像或光学相关断层扫描则突破单一成像局限，提升肝胆胰肿瘤诊断准确率。未来需聚焦红外二区探针生物安全性验证、动态给药模型优化，并通过多中心研究建立标准化操作流程，最终实现从病灶切除到器官功能保护的全周期精准外科革新。

总而言之，ICG荧光成像技术正经历从“解剖显影”到“功能-分子成像”的范式转变。未来需重点突破红外二区荧光探针的生物安全性、建立多中心标准化操作指南（如剂量-时机-显影评分体系），并通过真实世界研究验证其长期预后效益。随着人工智能与多模态影像的深度整合，该技术有望重塑精准外科的诊疗路径，实现从病灶切除到器官功能保护的全周期优化。

1、%E4%B8%AD%E5%9B%BD%E5%8C%BB%E5%B8%88%E5%8D%8F%E4%BC%9A%E5%A4%96%E7%A7%91%E5%8C%BB%E5%B8%88%E5%88%86%E4%BC%9A%E8%82%9D%E8%84%8F%E5%A4%96%E7%A7%91%E5%8C%BB%E5%B8%88%E5%A7%94%E5%91%98%E4%BC%9A%EF%BC%8E%E5%90%B2%E5%93%9A%E8%8F%81%E7%BB%BF%E8%8D%A7%E5%85%89%E6%88%90%E5%83%8F%E6%8A%80%E6%9C%AF%E5%9C%A8%E8%82%9D%E8%84%8F%E5%A4%96%E7%A7%91%E5%BA%94%E7%94%A8%E4%B8%AD%E5%9B%BD%E4%B8%93%E5%AE%B6%E5%85%B1%E8%AF%86%EF%BC%882023%E2%80%83%E7%89%88%EF%BC%89%EF%BC%BBJ%EF%BC%BD%EF%BC%8E%E4%B8%AD%E5%9B%BD%E5%AE%9E%E7%94%A8%E5%A4%96%E7%A7%91%E6%9D%82%E5%BF%97%EF%BC%8C2023%EF%BC%8C43%EF%BC%884%EF%BC%89%EF%BC%9A371-383%EF%BC%8E

2、中华医学会数字医学分会，中国医师协会肝癌专业委员会，中国医师协会精准医学专业委员会，等．吲哚菁绿分子荧光影像技术诊断原发性肝癌与术中导航操作诊疗规范（2021版）［J］．中国实用外科杂志，2021，41（9）：1002-1013，1032．

3、%E4%B8%AD%E5%8D%8E%E5%8C%BB%E5%AD%A6%E4%BC%9A%E6%95%B0%E5%AD%97%E5%8C%BB%E5%AD%A6%E5%88%86%E4%BC%9A%EF%BC%8C%E4%B8%AD%E5%9B%BD%E7%A0%94%E7%A9%B6%E5%9E%8B%E5%8C%BB%E9%99%A2%E5%AD%A6%E4%BC%9A%E6%95%B0%E5%AD%97%E6%99%BA%E8%83%BD%E5%8C%96%E5%A4%96%E7%A7%91%E4%B8%93%E4%B8%9A%E5%A7%94%E5%91%98%E4%BC%9A%EF%BC%8C%E4%B8%AD%E5%9B%BD%E5%8C%BB%E5%B8%88%E5%8D%8F%E4%BC%9A%E8%82%9D%E7%99%8C%E4%B8%93%E4%B8%9A%E5%A7%94%E5%91%98%E4%BC%9A%EF%BC%8C%E7%AD%89%EF%BC%8E%E8%AE%A1%E7%AE%97%E6%9C%BA%E8%BE%85%E5%8A%A9%E8%81%94%E5%90%88%E5%90%B2%E5%93%9A%E8%8F%81%E7%BB%BF%E5%88%86%E5%AD%90%E8%8D%A7%E5%85%89%E5%BD%B1%E5%83%8F%E6%8A%80%E6%9C%AF%E5%9C%A8%E8%82%9D%E8%84%8F%E8%82%BF%E7%98%A4%E8%AF%8A%E6%96%AD%E5%92%8C%E6%89%8B%E6%9C%AF%E5%AF%BC%E8%88%AA%E4%B8%AD%E5%BA%94%E7%94%A8%E6%8C%87%E5%8D%97%EF%BC%882019%E2%80%83%E7%89%88%EF%BC%89%EF%BC%BBJ%EF%BC%BD%EF%BC%8E%E4%B8%AD%E5%9B%BD%E5%AE%9E%E7%94%A8%E5%A4%96%E7%A7%91%E6%9D%82%E5%BF%97%EF%BC%8C2019%EF%BC%8C39%EF%BC%887%EF%BC%89%EF%BC%9A641-650%EF%BC%8C654%EF%BC%8E

4、中国研究型医院学会微创外科学专业委员会．吲哚菁绿荧光染色在腹腔镜肝切除术中应用的专家共识［J］．腹腔镜外科杂志，2019，24（5）：388-394．

5、中华医学会核医学分会，中华医学会数字医学分会，中国医师协会肝癌专业委员会，等．吲哚菁绿近红外二区荧光成像在肝癌切除术中应用的专家共识［J］．中华核医学与分子影像杂志，2023，43（7）：418-425．

6、WANG%E2%80%83X%EF%BC%8CTEH%E2%80%83C%E2%80%83S%E2%80%83C%EF%BC%8CISHIZAWA%E2%80%83T%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0AConsensus%E2%80%83%20guidelines%E2%80%83for%E2%80%83the%E2%80%83%20use%E2%80%83%20of%E2%80%83fluorescence%E2%80%83%0Aimaging%E2%80%83in%E2%80%83hepatobiliary%E2%80%83surgery%EF%BC%BBJ%EF%BC%BD%EF%BC%8EAnn%E2%80%83Surg%EF%BC%8C%0A2021%EF%BC%8C274%EF%BC%881%EF%BC%89%EF%BC%9A97-106%EF%BC%8E

7、SZAVAY%E2%80%83P%E2%80%83O%EF%BC%8CBONDOC%E2%80%83A%EF%BC%8CESPOSITO%E2%80%83C%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0AClinical%E2%80%83consensus%E2%80%83statement%E2%80%83on%E2%80%83the%E2%80%83use%E2%80%83of%E2%80%83indocyanine%E2%80%83%0Agreen%E2%80%83fluorescence-guided%E2%80%83surgery%E2%80%83in%E2%80%83pediatric%E2%80%83patients%0A%EF%BC%BBJ%EF%BC%BD%EF%BC%8EJ%E2%80%83Pediatr%E2%80%83Surg%EF%BC%8C2024%EF%BC%8C59%EF%BC%8811%EF%BC%89%EF%BC%9A161657%EF%BC%8E

8、de%E2%80%83MUYNCK%E2%80%83L%EF%BC%8CWHITE%E2%80%83K%E2%80%83P%EF%BC%8CALSEIDI%E2%80%83A%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0AConsensus%E2%80%83%20statement%E2%80%83%20on%E2%80%83the%E2%80%83%20use%E2%80%83%20of%E2%80%83%20near-infrared%E2%80%83%0Afluorescence%E2%80%83imaging%E2%80%83during%E2%80%83pancreatic%E2%80%83cancer%E2%80%83surgery%E2%80%83%0Abased%E2%80%83on%E2%80%83a%E2%80%83delphi%E2%80%83study%EF%BC%9ASurgeons%E2%80%99%E2%80%83%20perspectives%E2%80%83%0Aon%E2%80%83current%E2%80%83use%E2%80%83and%E2%80%83future%E2%80%83recommendations%EF%BC%BBJ%EF%BC%BD%EF%BC%8E%0ACancers%EF%BC%88Basel%EF%BC%89%EF%BC%8C2023%EF%BC%8C15%EF%BC%883%EF%BC%89%EF%BC%9A652%EF%BC%8E

9、CASSINOTTI%E2%80%83E%EF%BC%8CAL-TAHER%E2%80%83M%EF%BC%8CANTONIOU%E2%80%83S%E2%80%83A%EF%BC%8C%0Aet%E2%80%83al%EF%BC%8EEuropean%E2%80%83Association%E2%80%83for%E2%80%83Endoscopic%E2%80%83Surgery%0A%EF%BC%88EAES%EF%BC%89consensus%E2%80%83on%E2%80%83Indocyanine%E2%80%83Green%EF%BC%88ICG%EF%BC%89%0Afluorescence-guided%E2%80%83surgery%EF%BC%BBJ%EF%BC%BD%EF%BC%8ESurg%E2%80%83Endosc%EF%BC%8C%0A2023%EF%BC%8C37%EF%BC%883%EF%BC%89%EF%BC%9A1629-1648%EF%BC%8E

10、DIP%E2%80%83F%EF%BC%8CBONI%E2%80%83L%EF%BC%8CBOUVET%E2%80%83M%EF%BC%8Cet%E2%80%83al%EF%BC%8EConsensus%E2%80%83%0Aconference%E2%80%83statement%E2%80%83on%E2%80%83the%E2%80%83general%E2%80%83use%E2%80%83of%E2%80%83near%02infrared%E2%80%83fluorescence%E2%80%83imaging%E2%80%83and%E2%80%83indocyanine%E2%80%83green%E2%80%83%0Aguided%E2%80%83surgery%EF%BC%9AResults%E2%80%83of%E2%80%83a%E2%80%83modified%E2%80%83delphi%E2%80%83study%0A%EF%BC%BBJ%EF%BC%BD%EF%BC%8EAnn%E2%80%83Surg%EF%BC%8C2022%EF%BC%8C275%EF%BC%884%EF%BC%89%EF%BC%9A685-691%EF%BC%8E

11、刘付宝，蒋东，耿小平．吲哚菁绿荧光成像技术在胆道疾病中的应用［J］．中华消化外科杂志，2024，23（1）：80-84．

12、SATOU%E2%80%83S%EF%BC%8CISHIZAWA%E2%80%83T%EF%BC%8CMASUDA%E2%80%83K%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0AIndocyanine%E2%80%83green%E2%80%83fluorescent%E2%80%83imaging%E2%80%83for%E2%80%83%20detecting%E2%80%83%0Aextrahepatic%E2%80%83metastasis%E2%80%83of%E2%80%83hepatocellular%E2%80%83carcinoma%0A%EF%BC%BBJ%EF%BC%BD%EF%BC%8EJ%E2%80%83Gastroenterol%EF%BC%8C2013%EF%BC%8C48%EF%BC%8810%EF%BC%89%EF%BC%9A1136-%0A1143%EF%BC%8E

13、%E2%80%83%20ISHIZAWA%E2%80%83T%EF%BC%8CFUKUSHIMA%E2%80%83N%EF%BC%8CSHIBAHARA%E2%80%83J%EF%BC%8C%0Aet%E2%80%83al%EF%BC%8EReal-time%E2%80%83identification%E2%80%83%20of%E2%80%83liver%E2%80%83%20cancers%E2%80%83%20by%E2%80%83%0Ausing%E2%80%83indocyanine%E2%80%83green%E2%80%83fluorescent%E2%80%83imaging%EF%BC%BBJ%EF%BC%BD%EF%BC%8E%0ACancer%EF%BC%8C2009%EF%BC%8C115%EF%BC%8811%EF%BC%89%EF%BC%9A2491-2504%EF%BC%8E

14、MATSUMURA%E2%80%83M%EF%BC%8CSEYAMA%E2%80%83Y%EF%BC%8CISHIDA%E2%80%83H%EF%BC%8C%0Aet%E2%80%83al%EF%BC%8EIndocyanine%E2%80%83%20green%E2%80%83fluorescence%E2%80%83%20navigation%E2%80%83%0Afor%E2%80%83%20hepatocellular%E2%80%83%20carcinoma%E2%80%83%20with%E2%80%83%20bile%E2%80%83%20duct%E2%80%83tumor%E2%80%83%0Athrombus%EF%BC%9AA%E2%80%83case%E2%80%83report%EF%BC%BBJ%EF%BC%BD%EF%BC%8ESurg%E2%80%83Case%E2%80%83Rep%EF%BC%8C%0A2021%EF%BC%8C7%EF%BC%881%EF%BC%89%EF%BC%9A18%EF%BC%8E

15、KOKUDO%E2%80%83N%EF%BC%8CISHIZAWA%E2%80%83T%EF%BC%8EClinical%E2%80%83application%E2%80%83of%E2%80%83%0Afluorescence%E2%80%83imaging%E2%80%83of%E2%80%83liver%E2%80%83cancer%E2%80%83using%E2%80%83indocyanine%E2%80%83%0Agreen%EF%BC%BBJ%EF%BC%BD%EF%BC%8ELiver%E2%80%83Cancer%EF%BC%8C2012%EF%BC%8C1%EF%BC%881%EF%BC%89%EF%BC%9A15-21%EF%BC%8E

16、ISHIZAWA%E2%80%83T%EF%BC%8CMASUDA%E2%80%83K%EF%BC%8CURANO%E2%80%83Y%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0AMechanistic%E2%80%83%20background%E2%80%83%20and%E2%80%83%20clinical%E2%80%83%20applications%E2%80%83%0Aof%E2%80%83%20indocyanine%E2%80%83%20g%20reen%E2%80%83%20fluo%20rescence%E2%80%83%20imaging%E2%80%83%20of%E2%80%83%0Ahepatocellular%E2%80%83carcinoma%EF%BC%BBJ%EF%BC%BD%EF%BC%8EAnn%E2%80%83Surg%E2%80%83Oncol%EF%BC%8C%0A2014%EF%BC%8C21%EF%BC%882%EF%BC%89%EF%BC%9A440-448%EF%BC%8E

17、中华人民共和国国家卫生健康委员会医政司．原发性肝癌诊疗指南（2024年版）［J］．中国普通外科杂志，2024，33（4）：475-530．

18、HANDGRAAF%E2%80%83H%E2%80%83M%EF%BC%8CBOOGERD%E2%80%83L%E2%80%83F%EF%BC%8CHOPPENER%E2%80%83%0AD%E2%80%83J%EF%BC%8Cet%E2%80%83al%EF%BC%8ELong-term%E2%80%83follow-up%E2%80%83after%E2%80%83near-infrared%E2%80%83%0Afluorescence-guided%E2%80%83%20resection%E2%80%83%20of%E2%80%83%20colorectal%E2%80%83liver%E2%80%83%0Ametastases%EF%BC%9AA%E2%80%83retrospective%E2%80%83multicenter%E2%80%83analysis%EF%BC%BBJ%EF%BC%BD%EF%BC%8E%0AEur%E2%80%83J%E2%80%83Surg%E2%80%83Oncol%EF%BC%8C2017%EF%BC%8C43%EF%BC%888%EF%BC%89%EF%BC%9A1463-1471%EF%BC%8E

19、PELOSO%E2%80%83A%EF%BC%8CFRANCHI%E2%80%83E%EF%BC%8CCANEPA%E2%80%83M%E2%80%83C%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0ACombined%E2%80%83%20use%E2%80%83%20of%E2%80%83%20intraoperative%E2%80%83%20ultrasound%E2%80%83%20and%E2%80%83%0Aindocyanine%E2%80%83%20green%E2%80%83fluorescence%E2%80%83imaging%E2%80%83to%E2%80%83%20detect%E2%80%83%0Aliver%E2%80%83metastases%E2%80%83from%E2%80%83colorectal%E2%80%83cancer%EF%BC%BBJ%EF%BC%BD%EF%BC%8EHPB%0A%EF%BC%88Oxford%EF%BC%89%EF%BC%8C2013%EF%BC%8C15%EF%BC%8812%EF%BC%89%EF%BC%9A928-934%EF%BC%8E

20、AOKI%E2%80%83T%EF%BC%8CYASUDA%E2%80%83D%EF%BC%8CSHIMIZU%E2%80%83Y%EF%BC%8Cet%E2%80%83al%EF%BC%8EImage-guided%E2%80%83liver%E2%80%83mapping%E2%80%83%20using%E2%80%83fluorescence%E2%80%83%20navigation%E2%80%83system%E2%80%83with%E2%80%83indocyanine%E2%80%83green%E2%80%83for%E2%80%83anatomical%E2%80%83hepatic%E2%80%83%0Aresection%EF%BC%BBJ%EF%BC%BD%EF%BC%8EWorld%E2%80%83J%E2%80%83Surg%EF%BC%8C2008%EF%BC%8C32%EF%BC%888%EF%BC%89%EF%BC%9A%0A1763-1767%EF%BC%8E

21、%E2%80%83%20ISHIZAWA%E2%80%83T%EF%BC%8CSAIURA%E2%80%83A%EF%BC%8CKOKUDO%E2%80%83N%EF%BC%8EClinical%E2%80%83%0Aapplication%E2%80%83of%E2%80%83indocyanine%E2%80%83green-fluorescence%E2%80%83imaging%E2%80%83%0Aduring%E2%80%83hepatectomy%EF%BC%BBJ%EF%BC%BD%EF%BC%8EHepatobiliary%E2%80%83Surg%E2%80%83Nutr%EF%BC%8C%0A2016%EF%BC%8C5%EF%BC%884%EF%BC%89%EF%BC%9A322-328%EF%BC%8E

22、王宏光，许寅喆，陈明易，等．吲哚菁绿荧光融合影像引导在腹腔镜解剖性肝切除术中的应用价值［J］．中华消化外科杂志，2017，16（4）：405-409．

23、%E2%80%83%20KOBAYASHI%E2%80%83Y%EF%BC%8CKAWAGUCHI%E2%80%83Y%EF%BC%8CKOBAYASHI%E2%80%83%0AK%EF%BC%8Cet%E2%80%83al%EF%BC%8EPortal%E2%80%83%20vein%E2%80%83territory%E2%80%83identification%E2%80%83%20using%E2%80%83%0Aindocyanine%E2%80%83green%E2%80%83fluorescence%E2%80%83imaging%EF%BC%9ATechnical%E2%80%83%0Adetails%E2%80%83and%E2%80%83short-term%E2%80%83outcomes%EF%BC%BBJ%EF%BC%BD%EF%BC%8EJ%E2%80%83Surg%E2%80%83Oncol%EF%BC%8C%0A2017%EF%BC%8C116%EF%BC%887%EF%BC%89%EF%BC%9A921-931%EF%BC%8E

24、%E2%80%83%20MENG%E2%80%83X%EF%BC%8CWANG%E2%80%83H%EF%BC%8CXU%E2%80%83Y%EF%BC%8Cet%E2%80%83al%EF%BC%8EIndocyanine%E2%80%83%0Agreen%E2%80%83fluorescence%E2%80%83image-guided%E2%80%83total%E2%80%83laparoscopic%E2%80%83%0Aliving%E2%80%83donor%E2%80%83right%E2%80%83hepatectomy%EF%BC%9AThe%E2%80%83first%E2%80%83case%E2%80%83%20report%E2%80%83%0Afrom%E2%80%83Mainland%E2%80%83China%EF%BC%BBJ%EF%BC%BD%EF%BC%8EInt%E2%80%83J%E2%80%83Surg%E2%80%83Case%E2%80%83Rep%EF%BC%8C%0A2018%EF%BC%8853%EF%BC%89%EF%BC%9A406-409%EF%BC%8E

25、HU%E2%80%83M%EF%BC%8CCHEN%E2%80%83Z%EF%BC%8CXU%E2%80%83D%EF%BC%8Cet%E2%80%83al%EF%BC%8EEfficacy%E2%80%83%20and%E2%80%83%0Asafety%E2%80%83%20of%E2%80%83indocyanine%E2%80%83%20green%E2%80%83fluorescence%E2%80%83%20navigation%E2%80%83%0Aversus%E2%80%83%20conventional%E2%80%83laparoscopic%E2%80%83%20hepatectomy%E2%80%83for%E2%80%83%0Ahepatocellular%E2%80%83carcinoma%EF%BC%9AA%E2%80%83%20systematic%E2%80%83%20review%E2%80%83and%E2%80%83%0Ameta-analysis%EF%BC%BBJ%EF%BC%BD%EF%BC%8ESurg%E2%80%83Endosc%EF%BC%8C2025%EF%BC%8C39%0A%EF%BC%883%EF%BC%89%EF%BC%9A1681-1695%EF%BC%8E

26、%E2%80%83%20KINOSHITA%E2%80%83M%EF%BC%8CKAWAGUCHI%E2%80%83T%EF%BC%8CTANAKA%E2%80%83S%EF%BC%8C%0Aet%E2%80%83al%EF%BC%8EApplication%E2%80%83of%E2%80%83indocyanine%E2%80%83green%E2%80%83fluorescence%E2%80%83%0Aimaging%E2%80%83for%E2%80%83tumor%E2%80%83localization%E2%80%83%20during%E2%80%83%20robot-assisted%E2%80%83%0Ahepatectomy%EF%BC%BBJ%EF%BC%BD%EF%BC%8ECancers%EF%BC%88Basel%EF%BC%89%EF%BC%8C2023%EF%BC%8C15%0A%EF%BC%8817%EF%BC%89%EF%BC%9A4205%EF%BC%8E

27、%E2%80%83%20ROMPIANESI%E2%80%83G%EF%BC%8CPEGORARO%E2%80%83F%EF%BC%8CRAMACI%E2%80%83L%EF%BC%8C%0Aet%E2%80%83al%EF%BC%8EPreoperative%E2%80%83planning%E2%80%83and%E2%80%83intraoperative%E2%80%83real%02time%E2%80%83navigation%E2%80%83with%E2%80%83indocyanine%E2%80%83green%E2%80%83fluorescence%E2%80%83in%E2%80%83%0Arobotic%E2%80%83liver%E2%80%83surgery%EF%BC%BBJ%EF%BC%BD%EF%BC%8ELangenbecks%E2%80%83Arch%E2%80%83Surg%EF%BC%8C%0A2023%EF%BC%8C408%EF%BC%881%EF%BC%89%EF%BC%9A292%EF%BC%8E

28、KIM%E2%80%83J%E2%80%83H%EF%BC%8CPARK%E2%80%83H%E2%80%83M%EF%BC%8ED%20et%20a%20c%20h%20m%20e%20nt%20%E2%80%83%20of%20%E2%80%83%20t%20h%20e%E2%80%83%0AIntersegmental%E2%80%83%20Plane%E2%80%83in%E2%80%83Robotic%E2%80%83%20Left%E2%80%83%20Hepatectomy%E2%80%83%0AUsing%E2%80%83Indocyanine%E2%80%83Green%E2%80%83Fluorescent%E2%80%83Imaging%EF%BC%88with%E2%80%83%0AVideo%EF%BC%89%EF%BC%BBJ%EF%BC%BD%EF%BC%8EAnn%E2%80%83Surg%E2%80%83Oncol%EF%BC%8C2025%EF%BC%8C32%EF%BC%885%EF%BC%89%EF%BC%9A%0A3501-3504%EF%BC%8E

29、%E2%80%83%20KANEKO%E2%80%83J%EF%BC%8CKOKUDO%E2%80%83T%EF%BC%8CINAGAKI%E2%80%83Y%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0AInnovative%E2%80%83treatment%E2%80%83for%E2%80%83hepatocellular%E2%80%83carcinoma%0A%EF%BC%88HCC%EF%BC%89%EF%BC%BBJ%EF%BC%BD%EF%BC%8ETransl%E2%80%83Gastroenterol%E2%80%83Hepatol%EF%BC%8C2018%EF%BC%883%EF%BC%89%EF%BC%9A78%EF%BC%8E

30、SHIRATA%E2%80%83C%EF%BC%8CKANEKO%E2%80%83J%EF%BC%8CINAGAKI%E2%80%83Y%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0ANear-infrared%E2%80%83photothermal%2Fphotodynamic%E2%80%83therapy%E2%80%83with%E2%80%83%0Aindocyanine%E2%80%83green%E2%80%83induces%E2%80%83apoptosis%E2%80%83of%E2%80%83hepatocellular%E2%80%83%0Acarcinoma%E2%80%83cells%E2%80%83through%E2%80%83oxidative%E2%80%83stress%EF%BC%BBJ%EF%BC%BD%EF%BC%8ESci%E2%80%83%0ARep%EF%BC%8C2017%EF%BC%8C7%EF%BC%881%EF%BC%89%EF%BC%9A13958%EF%BC%8E

31、HE%E2%80%83P%EF%BC%8CTANG%E2%80%83H%EF%BC%8CZHENG%E2%80%83Y%EF%BC%8Cet%E2%80%83al%EF%BC%8EO%20ptical%E2%80%83%0Amolecular%E2%80%83imaging%E2%80%83technology%E2%80%83and%E2%80%83its%E2%80%83application%E2%80%83in%E2%80%83%0Aprecise%E2%80%83surgical%E2%80%83navigation%E2%80%83of%E2%80%83liver%E2%80%83cancer%EF%BC%BBJ%EF%BC%BD%EF%BC%8E%0ATheranostics%EF%BC%8C2025%EF%BC%8C15%EF%BC%883%EF%BC%89%EF%BC%9A1017-1034%EF%BC%8E

32、NAKAMURA%E2%80%83G%EF%BC%8CASAI%E2%80%83K%EF%BC%8CWATANABE%E2%80%83R%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0ATwo%E2%80%83cases%E2%80%83of%E2%80%83laparoscopic%E2%80%83deroofing%E2%80%83of%E2%80%83giant%E2%80%83liver%E2%80%83cysts%E2%80%83%0Ausing%E2%80%83indocyanine%E2%80%83green%E2%80%83fluorescence%E2%80%83imaging%EF%BC%BBJ%EF%BC%BD%EF%BC%8E%0AAsian%E2%80%83J%E2%80%83Endosc%E2%80%83Surg%EF%BC%8C2024%EF%BC%8C17%EF%BC%883%EF%BC%89%EF%BC%9Ae13308%EF%BC%8E

33、LIU%E2%80%83H%EF%BC%8CKUANG%E2%80%83J%EF%BC%8CHUANG%E2%80%83Y%EF%BC%8Cet%E2%80%83al%EF%BC%8EIndocyanine%E2%80%83%0Agreen%E2%80%83fluorescence%E2%80%83%E2%80%93%E2%80%83%20guided%E2%80%83%20avoidance%E2%80%83%20of%E2%80%83%20bile%E2%80%83%20duct%E2%80%83%0Ainjury%E2%80%83%20during%E2%80%83%20simultaneous%E2%80%83laparoscopic%E2%80%83fenestration%E2%80%83%0Aand%E2%80%83cholecystectomy%EF%BC%BBJ%EF%BC%BD%EF%BC%8EClin%E2%80%83Med%E2%80%83Res%EF%BC%8C2022%EF%BC%8C11%0A%EF%BC%885%EF%BC%89%EF%BC%9A145-149%EF%BC%8E

34、UNE%E2%80%83N%EF%BC%8CFUJIO%E2%80%83A%EF%BC%8CMITSUGASHIRA%E2%80%83H%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0ALaparoscopic%E2%80%83liver%E2%80%83%20cyst%E2%80%83fenestration%E2%80%83with%E2%80%83%20real-time%E2%80%83%0Aindocyanine%E2%80%83green%E2%80%83fluorescence-guided%E2%80%83surgery%EF%BC%9A%0AA%E2%80%83case%E2%80%83report%EF%BC%BBJ%EF%BC%BD%EF%BC%8EJ%E2%80%83Surg%E2%80%83Case%E2%80%83Rep%EF%BC%8C2021%EF%BC%8C2021%0A%EF%BC%885%EF%BC%89%EF%BC%9Arjab196%EF%BC%8E

35、SHIMAGAKI%E2%80%83T%EF%BC%8CITOH%E2%80%83S%EF%BC%8CTOSHIDA%E2%80%83K%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0APrevention%E2%80%83of%E2%80%83bile%E2%80%83duct%E2%80%83injury%E2%80%83using%E2%80%83indocyanine%E2%80%83green%E2%80%83%0Afluorescence%E2%80%83in%E2%80%83laparoscopic%E2%80%83liver%E2%80%83cyst%E2%80%83fenestration%E2%80%83for%E2%80%83%0Agiant%E2%80%83liver%E2%80%83cyst%EF%BC%9AA%E2%80%83case%E2%80%83report%EF%BC%BBJ%EF%BC%BD%EF%BC%8EJ%E2%80%83%20Surg%E2%80%83Case%E2%80%83%0ARep%EF%BC%8C2022%EF%BC%8C2022%EF%BC%8810%EF%BC%89%EF%BC%9Arjac479%EF%BC%8E

36、HAVERINEN%E2%80%83S%EF%BC%8CPAJUS%E2%80%83E%EF%BC%8CSANDBLOM%E2%80%83G%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0AIndocyanine%E2%80%83%20green%E2%80%83fluorescence%E2%80%83improves%E2%80%83%20safety%E2%80%83in%E2%80%83%0Alaparoscopic%E2%80%83cholecystectomy%E2%80%83%20using%E2%80%83the%E2%80%83Fundus%E2%80%83First%E2%80%83%0Atechnique%EF%BC%9AA%E2%80%83retrospective%E2%80%83study%EF%BC%BBJ%EF%BC%BD%EF%BC%8EFront%E2%80%83Surg%EF%BC%8C%0A2025%EF%BC%8812%EF%BC%89%EF%BC%9A1516709%EF%BC%8E

37、%E2%80%83%20ISHIZAWA%E2%80%83T%EF%BC%8CBANDAI%E2%80%83Y%EF%BC%8CIJICHI%E2%80%83M%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0AFluorescent%E2%80%83cholangiography%E2%80%83illuminating%E2%80%83the%E2%80%83%20biliary%E2%80%83%0Atree%E2%80%83during%E2%80%83laparoscopic%E2%80%83cholecystectomy%EF%BC%BBJ%EF%BC%BD%EF%BC%8EBr%E2%80%83J%E2%80%83%0ASurg%EF%BC%8C2010%EF%BC%8C97%EF%BC%889%EF%BC%89%EF%BC%9A1369-1377%EF%BC%8E

38、JIN%E2%80%83H%EF%BC%8CYANG%E2%80%83J%EF%BC%8CLU%E2%80%83L%EF%BC%8Cet%E2%80%83al%EF%BC%8EP%20r%20o%20p%20e%20n%20sit%20y%E2%80%83%0Ascore%E2%80%83%20matching%E2%80%83%20between%E2%80%83%20conventional%E2%80%83laparoscopic%E2%80%83%0Acholecystectomy%E2%80%83and%E2%80%83indocyanine%E2%80%83green%E2%80%83cholangiography%02guided%E2%80%83laparoscopic%E2%80%83cholecystectomy%EF%BC%9AObservational%E2%80%83%0Astudy%EF%BC%BBJ%EF%BC%BD%EF%BC%8ELasers%E2%80%83Med%E2%80%83Sci%EF%BC%8C2022%EF%BC%8C37%EF%BC%882%EF%BC%89%EF%BC%9A%0A1351-1359%EF%BC%8E

39、%E2%80%83%20van%E2%80%83den%E2%80%83BOS%E2%80%83J%EF%BC%8CSCHOLS%E2%80%83R%E2%80%83M%EF%BC%8CBONI%E2%80%83L%EF%BC%8Cet%E2%80%83al%EF%BC%8ENear-infrared%E2%80%83fluorescence%E2%80%83cholangiography%E2%80%83assisted%E2%80%83%0Alaparoscopic%E2%80%83cholecystectomy%EF%BC%88FALCON%EF%BC%89%EF%BC%9AAn%E2%80%83%0Ainternational%E2%80%83multicentre%E2%80%83randomized%E2%80%83controlled%E2%80%83trial%0A%EF%BC%BBJ%EF%BC%BD%EF%BC%8ESurg%E2%80%83Endosc%EF%BC%8C2023%EF%BC%8C37%EF%BC%886%EF%BC%89%EF%BC%9A4574-4584%EF%BC%8E

40、CASTAGNETO-GISSEY%E2%80%83L%EF%BC%8CRUSSO%E2%80%83M%E2%80%83F%EF%BC%8CIODICE%E2%80%83%0AA%EF%BC%8Cet%E2%80%83al%EF%BC%8EInt%20racholecystic%E2%80%83%20ve%20rsus%E2%80%83%20Int%20ravenous%E2%80%83%0AIndocyanine%E2%80%83Green%EF%BC%88ICG%EF%BC%89injection%E2%80%83for%E2%80%83biliary%E2%80%83anatomy%E2%80%83%0Aevaluation%E2%80%83%20by%E2%80%83fluorescent%E2%80%83%20cholangiography%E2%80%83%20during%E2%80%83%0Alaparoscopic%E2%80%83cholecystectomy%EF%BC%9AA%E2%80%83case-control%E2%80%83study%0A%EF%BC%BBJ%EF%BC%BD%EF%BC%8EJ%E2%80%83Clin%E2%80%83Med%EF%BC%8C2022%EF%BC%8C11%EF%BC%8812%EF%BC%89%EF%BC%9A3508%EF%BC%8E

41、ZHOU%E2%80%83H%EF%BC%8CHE%E2%80%83Y%EF%BC%8CCHANG%E2%80%83L%EF%BC%8EComparative%E2%80%83%20study%E2%80%83%0Aof%E2%80%83%20Indocyanine%E2%80%83Green%E2%80%83fluorescence%E2%80%83imaging%E2%80%83%20versus%E2%80%83%0Aconventional%E2%80%83fiber-optic%E2%80%83imaging%E2%80%83in%E2%80%83laparoscopic%E2%80%83%0Acholedochotomy%E2%80%83for%E2%80%83%20stone%E2%80%83%20extraction%E2%80%83%20and%E2%80%83immediate%E2%80%83%0Abiliary%E2%80%83incision%E2%80%83suturing%EF%BC%BBJ%EF%BC%BD%EF%BC%8EUpdates%E2%80%83Surg%EF%BC%8C2024%EF%BC%8C%0A76%EF%BC%886%EF%BC%89%EF%BC%9A2247-2254%EF%BC%8E

42、%E2%80%83%20HUANG%E2%80%83H%EF%BC%8CDU%E2%80%83D%EF%BC%8CWANG%E2%80%83Z%EF%BC%8Cet%E2%80%83al%EF%BC%8EApplication%E2%80%83of%E2%80%83%0Aintraoperative%E2%80%83fluorescence%E2%80%83imaging%E2%80%83with%E2%80%83indocyanine%E2%80%83%0Agreen%E2%80%83in%E2%80%83the%E2%80%83difficult%E2%80%83gallbladder%EF%BC%9AA%E2%80%83%20comparative%E2%80%83%0Astudy%E2%80%83between%E2%80%83indocyanine%E2%80%83green-guided%E2%80%83fluorescence%E2%80%83%0Acholangiography%E2%80%83and%E2%80%83conventional%E2%80%83surgery%EF%BC%BBJ%EF%BC%BD%EF%BC%8EJ%E2%80%83%0ALaparoendosc%E2%80%83Adv%E2%80%83Surg%E2%80%83Tech%E2%80%83A%EF%BC%8C2023%EF%BC%8C33%EF%BC%884%EF%BC%89%EF%BC%9A%0A404-410%EF%BC%8E

43、陈志，吴宏匀，朱国栋，等．经胆囊注射吲哚菁绿在腹腔镜困难型胆囊切除术中的应用［J］．肝胆胰外科杂志，2023，35（9）：530-535．

44、ZHANG%E2%80%83L%EF%BC%8CLIU%E2%80%83X%EF%BC%8CZOU%E2%80%83B%EF%BC%8Cet%E2%80%83al%EF%BC%8EThe%E2%80%83%20role%E2%80%83%20of%E2%80%83%0Aindocyanine%E2%80%83green%E2%80%83fluorescence%E2%80%83in%E2%80%83complex%E2%80%83laparoscopic%E2%80%83%0Acholecystectomy%E2%80%83navigation%EF%BC%BBJ%EF%BC%BD%EF%BC%8EJ%E2%80%83Vis%E2%80%83Exp%EF%BC%8C2025%0A%EF%BC%88215%EF%BC%89%EF%BC%8E

45、T%E2%80%83K%E2%80%83J%EF%BC%8CRATHOD%E2%80%83K%E2%80%83J%EF%BC%8CSAXENA%E2%80%83R%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0AIndocyanine%E2%80%83%20green%E2%80%83fluorescence-guided%E2%80%83%20surgery%E2%80%83in%E2%80%83%0Apediatric%E2%80%83hepatobiliary%E2%80%83procedures%EF%BC%9AA%E2%80%83feasibility%E2%80%83study%E2%80%83%0Afor%E2%80%83improved%E2%80%83intraoperative%E2%80%83visualization%EF%BC%BBJ%EF%BC%BD%EF%BC%8EEur%E2%80%83J%E2%80%83%0APediatr%E2%80%83Surg%EF%BC%8C2025%EF%BC%8C35%EF%BC%884%EF%BC%89%EF%BC%9A277-285%EF%BC%8E

46、CHEN%E2%80%83Q%EF%BC%8CZHOU%E2%80%83R%EF%BC%8CWENG%E2%80%83J%EF%BC%8Cet%E2%80%83al%EF%BC%8EExtrahepatic%E2%80%83%0Abilia%20ry%E2%80%83%20t%20ract%E2%80%83%20visualization%E2%80%83%20using%E2%80%83%20nea%20r-inf%20ra%20red%E2%80%83%0Afluorescence%E2%80%83imaging%E2%80%83with%E2%80%83indocyanine%E2%80%83green%EF%BC%9A%0AOptimization%E2%80%83of%E2%80%83dose%E2%80%83and%E2%80%83dosing%E2%80%83time%EF%BC%BBJ%EF%BC%BD%EF%BC%8ESurg%E2%80%83%0AEndosc%EF%BC%8C2021%EF%BC%8C35%EF%BC%8810%EF%BC%89%EF%BC%9A5573-5582%EF%BC%8E

47、HUANG%E2%80%83Y%EF%BC%8CCHEN%E2%80%83Q%EF%BC%8CKUANG%E2%80%83J%EF%BC%8Cet%E2%80%83al%EF%BC%8EReal%02time%E2%80%83fluorescent%E2%80%83%20cholangiography%E2%80%83with%E2%80%83indocyanine%E2%80%83%0Agreen%E2%80%83in%E2%80%83laparoscopic%E2%80%83cholecystectomy%EF%BC%9AA%E2%80%83%20randomized%E2%80%83%0Acontrolled%E2%80%83trial%E2%80%83to%E2%80%83establish%E2%80%83the%E2%80%83optimal%E2%80%83indocyanine%E2%80%83%0Agreen%E2%80%83dose%E2%80%83within%E2%80%8330min%E2%80%83preoperatively%EF%BC%BBJ%EF%BC%BD%EF%BC%8ESurg%E2%80%83today%EF%BC%8C2023%EF%BC%8C53%EF%BC%882%EF%BC%89%EF%BC%9A223-231%EF%BC%8E

48、%E2%80%83%20LIU%E2%80%83H%EF%BC%8CKUANG%E2%80%83J%EF%BC%8CXU%E2%80%83Y%EF%BC%8Cet%E2%80%83al%EF%BC%8EInvestigation%E2%80%83of%E2%80%83the%E2%80%83%0Aoptimal%E2%80%83indocyanine%E2%80%83green%E2%80%83dose%E2%80%83in%E2%80%83real-time%E2%80%83fluorescent%E2%80%83%0Acholangiography%E2%80%83during%E2%80%83laparoscopic%E2%80%83cholecystectomy%E2%80%83%0Awith%E2%80%83an%E2%80%83ultra-high-definition%E2%80%834K%E2%80%83fluorescent%E2%80%83system%EF%BC%9A%0AA%E2%80%83randomized%E2%80%83controlled%E2%80%83trial%EF%BC%BBJ%EF%BC%BD%EF%BC%8EUpdates%E2%80%83Surg%EF%BC%8C%0A2023%EF%BC%8C75%EF%BC%887%EF%BC%89%EF%BC%9A1903-1910%EF%BC%8E

49、张中林，刘符生，袁玉峰．吲哚菁绿荧光技术在肝胆外科应用中的几个关键问题再认识［J］．腹部外科，2022，35（4）：241-244，257．

50、张红裔，吕洪亮，杨聚鹏，等．吲哚菁绿荧光成像技术在腹腔镜胆囊切除术中的应用［J］．肝胆胰外科杂志，2024，36（1）：36-40．

51、黄宇，翁杰锋．吲哚菁绿荧光显影术中胆漏1例并文献复习［J］．腹腔镜外科杂志，2024，29（12）：955-957．

52、HANAKI%E2%80%83T%EF%BC%8CTOKUYASU%E2%80%83N%EF%BC%8CSAKAMOTO%E2%80%83T%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0AHepatectomy%E2%80%83guided%E2%80%83by%E2%80%83indocyanine%E2%80%83green%E2%80%83fluorescent%E2%80%83%0Aimaging%E2%80%83for%E2%80%83visualizing%E2%80%83bile%E2%80%83leakage%EF%BC%88with%E2%80%83video%EF%BC%89%EF%BC%BBJ%EF%BC%BD%EF%BC%8E%0AClin%E2%80%83Case%E2%80%83Rep%EF%BC%8C2022%EF%BC%8C10%EF%BC%886%EF%BC%89%EF%BC%9Ae05942%EF%BC%8E

53、HANAKI%E2%80%83T%EF%BC%8CGOTO%E2%80%83K%EF%BC%8CMORIMOTO%E2%80%83M%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0ASurgical%E2%80%83%20administration%E2%80%83%20of%E2%80%83%20indocyanine%E2%80%83%20green%E2%80%83%20in%E2%80%83%0Ahepatectomy%E2%80%83for%E2%80%83improved%E2%80%83bile%E2%80%83leakage%E2%80%83detection%EF%BC%BBJ%EF%BC%BD%EF%BC%8E%0AAnticancer%E2%80%83Res%EF%BC%8C2022%EF%BC%8C42%EF%BC%8810%EF%BC%89%EF%BC%9A4787-4793%EF%BC%8E

54、HUAN%E2%80%83G%E2%80%83Y%EF%BC%8CLI%E2%80%83T%E2%80%83Y%EF%BC%8CWENG%E2%80%83J%E2%80%83F%EF%BC%8Cet%E2%80%83al%EF%BC%8EPeritoneal%E2%80%83%0Afluid%E2%80%83indocyanine%E2%80%83green%E2%80%83test%E2%80%83for%E2%80%83diagnosis%E2%80%83of%E2%80%83gut%E2%80%83leakage%E2%80%83%0Ain%E2%80%83%20anastomotic%E2%80%83leakage%E2%80%83%20rats%E2%80%83%20and%E2%80%83%20colorectal%E2%80%83%20surgery%E2%80%83%0Apatients%EF%BC%BBJ%EF%BC%BD%EF%BC%8EWorld%E2%80%83J%E2%80%83Gastrointest%E2%80%83Surg%EF%BC%8C2024%EF%BC%8C16%0A%EF%BC%886%EF%BC%89%EF%BC%9A1825-1834%EF%BC%8E

55、%E2%80%83%20LEE%E2%80%83J%EF%BC%8CCHOI%E2%80%83Y%EF%BC%8CYI%E2%80%83N%E2%80%83J%EF%BC%8Cet%E2%80%83al%EF%BC%8EFea%20sibility%E2%80%83%0Aof%E2%80%83%20indocyanine%E2%80%83%20g%20reen%E2%80%83%20fluo%20rescence%E2%80%83%20imaging%E2%80%83%20to%E2%80%83%0Ap%20re%20dict%E2%80%83%20bilia%20ry%E2%80%83%20com%20plicatio%20n%20s%E2%80%83%20i%20n%E2%80%83%20livi%20ng%E2%80%83%20do%20no%20r%E2%80%83%0Aliver%E2%80%83transplantation%EF%BC%9AA%E2%80%83pilot%E2%80%83study%EF%BC%BBJ%EF%BC%BD%EF%BC%8EAnn%E2%80%83%0AHepatobiliary%E2%80%83Pancreat%E2%80%83Surg%EF%BC%8C2025%EF%BC%8C29%EF%BC%881%EF%BC%89%EF%BC%9A32-37%EF%BC%8E

56、%E2%80%83%20HUAN%E2%80%83Z%EF%BC%8CZENG%E2%80%83S%EF%BC%8CWEN%E2%80%83S%EF%BC%8Cet%E2%80%83al%EF%BC%8EFluorescence%02guided%E2%80%83visualization%E2%80%83of%E2%80%83pancreatic%E2%80%83neuroendocrine%E2%80%83tumor%E2%80%83%0Aborders%EF%BC%BBJ%EF%BC%BD%EF%BC%8EThe%E2%80%83Innovation%E2%80%83Medicine%EF%BC%8C2023%EF%BC%8C1%0A%EF%BC%882%EF%BC%89%EF%BC%9A100%E2%80%83025%EF%BC%8E

57、%E2%80%83%20TAO%E2%80%83H%EF%BC%8CZENG%E2%80%83X%EF%BC%8CLIN%E2%80%83W%EF%BC%8Cet%E2%80%83al%EF%BC%8EIndocyanine%E2%80%83%0Agreen%E2%80%83fluorescence%E2%80%83imaging%E2%80%83to%E2%80%83localize%E2%80%83insulinoma%E2%80%83and%E2%80%83%0Aprovide%E2%80%83three-dimensional%E2%80%83demarcation%E2%80%83for%E2%80%83laparoscopic%E2%80%83%0Aenucleation%EF%BC%9Aa%E2%80%83retrospective%E2%80%83single-arm%E2%80%83cohort%E2%80%83study%0A%EF%BC%BBJ%EF%BC%BD%EF%BC%8EInt%E2%80%83J%E2%80%83Surg%EF%BC%8C2023%EF%BC%8C109%EF%BC%884%EF%BC%89%EF%BC%9A821-828%EF%BC%8E

58、%E2%80%83%20LI%E2%80%83Z%EF%BC%8CLI%E2%80%83Z%EF%BC%8CRAMOS%E2%80%83A%EF%BC%8Cet%E2%80%83al%EF%BC%8EDetectio%20n%E2%80%83%20of%E2%80%83%0Apancreatic%E2%80%83%20cancer%E2%80%83%20by%E2%80%83indocyanine%E2%80%83%20green-assisted%E2%80%83fluorescence%E2%80%83imaging%E2%80%83in%E2%80%83the%E2%80%83first%E2%80%83and%E2%80%83second%E2%80%83near%02infrared%E2%80%83windows%EF%BC%BBJ%EF%BC%BD%EF%BC%8ECancer%E2%80%83Commun%EF%BC%88Lond%EF%BC%89%EF%BC%8C%0A2021%EF%BC%8C41%EF%BC%8812%EF%BC%89%EF%BC%9A1431-1434%EF%BC%8E

59、ZHOU%E2%80%83L%EF%BC%8CDAI%E2%80%83M%EF%BC%8CZHOU%E2%80%83J%EF%BC%8Cet%E2%80%83al%EF%BC%8EActive-targeted%E2%80%83%0AICG%E2%80%83for%E2%80%83%20surgical%E2%80%83%20navigation%E2%80%83and%E2%80%83fluorescence-guided%E2%80%83%0Alaparoscopic%E2%80%83photothermal%E2%80%83ablation%E2%80%83in%E2%80%83pancreatic%E2%80%83ductal%E2%80%83%0Aadenocarcinoma%EF%BC%BBJ%EF%BC%BD%EF%BC%8EAnal%E2%80%83Chem%EF%BC%8C2025%EF%BC%8C97%0A%EF%BC%881%EF%BC%89%EF%BC%9A473-481%EF%BC%8E

60、%E2%80%83%20REED%E2%80%83M%E2%80%83S%EF%BC%8COCHOA%E2%80%83M%EF%BC%8CTICHAUER%E2%80%83K%E2%80%83M%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0AMapping%E2%80%83%20estimates%E2%80%83%20of%E2%80%83%20vascular%E2%80%83%20permeability%E2%80%83with%E2%80%83%20a%E2%80%83%0Aclinical%E2%80%83indocyanine%E2%80%83green%E2%80%83fluorescence%E2%80%83imaging%E2%80%83system%E2%80%83%0Ain%E2%80%83experimental%E2%80%83pancreatic%E2%80%83adenocarcinoma%E2%80%83tumors%0A%EF%BC%BBJ%EF%BC%BD%EF%BC%8EJ%E2%80%83Biomed%E2%80%83Opt%EF%BC%8C2023%EF%BC%8C28%EF%BC%887%EF%BC%89%EF%BC%9A076001%EF%BC%8E

61、%E2%80%83%20GHIMIRE%E2%80%83R%EF%BC%8CLIMBU%E2%80%83Y%EF%BC%8CREGMEE%E2%80%83S%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0AIndocyanine%E2%80%83green%E2%80%83fluorescence%E2%80%83imaging%EF%BC%9AAssessment%E2%80%83%0Aof%E2%80%83%20perfusion%E2%80%83%20at%E2%80%83%20pancreatic%E2%80%83%20resection%E2%80%83%20margin%E2%80%83%20during%E2%80%83%0Apancreatoduodenectomy%EF%BC%9AA%E2%80%83cross%E2%80%83sectional%E2%80%83study%EF%BC%BBJ%EF%BC%BD%EF%BC%8E%0AHealth%E2%80%83Sci%E2%80%83Rep%EF%BC%8C2024%EF%BC%8C7%EF%BC%8810%EF%BC%89%EF%BC%9Ae70153%EF%BC%8E

62、%E2%80%83%20CHEN%E2%80%83J%E2%80%83W%EF%BC%8CLOF%E2%80%83S%EF%BC%8CZWART%E2%80%83M%E2%80%83J%E2%80%83W%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0AIntraoperative%E2%80%83fluorescence%E2%80%83imaging%E2%80%83%20during%E2%80%83%20robotic%E2%80%83%0Apancreatoduodenectomy%E2%80%83to%E2%80%83%20detect%E2%80%83%20suture-induced%E2%80%83%0Ahypoperfusion%E2%80%83of%E2%80%83the%E2%80%83%20pancreatic%E2%80%83%20stump%E2%80%83as%E2%80%83a%E2%80%83%20predictor%E2%80%83%0Aof%E2%80%83postoperative%E2%80%83pancreatic%E2%80%83fistula%EF%BC%88FLUOPAN%EF%BC%89%EF%BC%9A%0AProspective%E2%80%83proof-of-concept%E2%80%83study%EF%BC%BBJ%EF%BC%BD%EF%BC%8EAnn%E2%80%83Surg%E2%80%83%0AOpen%EF%BC%8C2023%EF%BC%8C4%EF%BC%884%EF%BC%89%EF%BC%9Ae354%EF%BC%8E

63、ROBERTSON%E2%80%83F%E2%80%83P%EF%BC%8CSPIERS%E2%80%83H%E2%80%83V%E2%80%83M%EF%BC%8CLIM%E2%80%83W%E2%80%83B%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0AIntraoperative%E2%80%83%20pancreas%E2%80%83%20stump%E2%80%83%20perfusion%E2%80%83assessment%E2%80%83%0Aduring%E2%80%83pancreaticoduodenectomy%EF%BC%9AA%E2%80%83%20systematic%E2%80%83%0Ascoping%E2%80%83review%EF%BC%BBJ%EF%BC%BD%EF%BC%8EWorld%E2%80%83J%E2%80%83Gastrointest%E2%80%83Surg%EF%BC%8C%0A2023%EF%BC%8C15%EF%BC%888%EF%BC%89%EF%BC%9A1799-1807%EF%BC%8E

64、HUANG%E2%80%83J%EF%BC%8CHU%E2%80%83W%EF%BC%8CLIU%E2%80%83J%EF%BC%8Cet%E2%80%83al%EF%BC%8EReal-time%E2%80%83%0Afluorescence%E2%80%83imaging%E2%80%83with%E2%80%83indocyanine%E2%80%83green%E2%80%83%20during%E2%80%83%0Alaparoscopic%E2%80%83%20duodenum-preserving%E2%80%83%20pancreatic%E2%80%83%20head%E2%80%83%0Aresection%EF%BC%BBJ%EF%BC%BD%EF%BC%8EPancreatology%EF%BC%8C2024%EF%BC%8C24%EF%BC%881%EF%BC%89%EF%BC%9A%0A130-136%EF%BC%8E

65、%E2%80%83LU%E2%80%83C%EF%BC%8CXU%E2%80%83B%EF%BC%8CMOU%E2%80%83Y%EF%BC%8Cet%E2%80%83al%EF%BC%8ELa%20pa%20ro%20sco%20pic%E2%80%83%0Aduodenum-preserving%E2%80%83pancreatic%E2%80%83head%E2%80%83%20resection%E2%80%83with%E2%80%83%0Areal-time%E2%80%83indocyanine%E2%80%83%20green%E2%80%83%20guidance%E2%80%83%20of%E2%80%83%20different%E2%80%83%0Adosage%E2%80%83and%E2%80%83timing%EF%BC%9AEnhanced%E2%80%83%20safety%E2%80%83with%E2%80%83visualized%E2%80%83biliary%E2%80%83duct%E2%80%83and%E2%80%83its%E2%80%83long-term%E2%80%83metabolic%E2%80%83morbidity%EF%BC%BBJ%EF%BC%BD%EF%BC%8E%0ALangenbecks%E2%80%83Arch%E2%80%83Surg%EF%BC%8C2022%EF%BC%8C407%EF%BC%887%EF%BC%89%EF%BC%9A2823-%0A2832%EF%BC%8E

66、%E2%80%83%20WANG%E2%80%83X%EF%BC%8CTENG%E2%80%83X%EF%BC%8CLIU%E2%80%83Y%EF%BC%8Cet%E2%80%83al%EF%BC%8EIndocyanine%E2%80%83%0Agreen%E2%80%83%20fluorescence-guided%E2%80%83%20laparoscopic%E2%80%83%20central%E2%80%83%0Apancreatectomy%E2%80%83for%E2%80%83%20complete%E2%80%83%20pancreatic%E2%80%83transection%E2%80%83%0Atrauma%EF%BC%9AA%E2%80%83rare%E2%80%83case%E2%80%83and%E2%80%83literature%E2%80%83review%EF%BC%BBJ%EF%BC%BD%EF%BC%8EFront%E2%80%83%0ASurg%EF%BC%8C2024%EF%BC%8811%EF%BC%89%EF%BC%9A1448064%EF%BC%8E

67、WAKABAYASHI%E2%80%83T%EF%BC%8CCACCIAGUERRA%E2%80%83A%E2%80%83B%EF%BC%8CABE%E2%80%83%0AY%EF%BC%8Cet%E2%80%83al%EF%BC%8EIndocyanine%E2%80%83green%E2%80%83fluorescence%E2%80%83navigation%E2%80%83%0Ain%E2%80%83liver%E2%80%83surgery%EF%BC%9AA%E2%80%83%20systematic%E2%80%83%20review%E2%80%83%20on%E2%80%83%20dose%E2%80%83%20and%E2%80%83%0Atiming%E2%80%83of%E2%80%83administration%EF%BC%BBJ%EF%BC%BD%EF%BC%8EAnn%E2%80%83Surg%EF%BC%8C2022%EF%BC%8C275%0A%EF%BC%886%EF%BC%89%EF%BC%9A1025-1034%EF%BC%8E

68、%E2%80%83%20KOSE%E2%80%83E%EF%BC%8CKAHRAMANGIL%E2%80%83B%EF%BC%8CAYDIN%E2%80%83H%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0AA%E2%80%83comparison%E2%80%83of%E2%80%83indocyanine%E2%80%83green%E2%80%83fluorescence%E2%80%83and%E2%80%83%0Alaparoscopic%E2%80%83ultrasound%E2%80%83for%E2%80%83detection%E2%80%83of%E2%80%83liver%E2%80%83tumors%0A%EF%BC%BBJ%EF%BC%BD%EF%BC%8EHPB%EF%BC%88Oxford%EF%BC%89%EF%BC%8C2020%EF%BC%8C22%EF%BC%885%EF%BC%89%EF%BC%9A764-769%EF%BC%8E

69、MASUDA%E2%80%83K%EF%BC%8CKANEKO%E2%80%83J%EF%BC%8CKAWAGUCHI%E2%80%83Y%EF%BC%8Cet%E2%80%83al%EF%BC%8E%0ADiagnostic%E2%80%83accuracy%E2%80%83of%E2%80%83indocyanine%E2%80%83green%E2%80%83fluorescence%E2%80%83%0Aimaging%E2%80%83and%E2%80%83multidetector%E2%80%83row%E2%80%83computed%E2%80%83tomography%E2%80%83for%E2%80%83%0Aidentifying%E2%80%83hepatocellular%E2%80%83carcinoma%E2%80%83with%E2%80%83liver%E2%80%83explant%E2%80%83%0Acorrelation%EF%BC%BBJ%EF%BC%BD%EF%BC%8EHepatol%E2%80%83Res%EF%BC%8C2017%EF%BC%8C47%EF%BC%8812%EF%BC%89%EF%BC%9A%0A1299-1307%EF%BC%8E

70、%E6%A2%81%E9%9C%84%EF%BC%8C%E7%BF%9F%E6%B7%91%E4%BA%AD%EF%BC%8C%E6%A2%81%E5%B2%B3%E9%BE%99%EF%BC%8C%E7%AD%89%EF%BC%8E%E8%8D%A7%E5%85%89%E5%AF%BC%E8%88%AA%E8%85%B9%E8%85%94%E9%95%9C%E8%82%9D%E8%84%8F%E8%82%BF%E7%98%A4%E5%88%87%E9%99%A4%E5%90%B2%E5%93%9A%E8%8F%81%E7%BB%BF%E6%9C%AF%E5%89%8D%E7%BB%99%E8%8D%AF%E6%97%B6%E6%9C%BA%EF%BC%9A%E5%8D%95%E4%B8%AD%E5%BF%8360%E4%BE%8B%E7%BB%8F%E9%AA%8C%EF%BC%BBJ%EF%BC%BD%EF%BC%8E%E4%B8%AD%E5%8D%8E%E8%82%9D%E8%83%86%E5%A4%96%E7%A7%91%E6%9D%82%E5%BF%97%EF%BC%8C2019%EF%BC%8C25%EF%BC%882%EF%BC%89%EF%BC%9A90-93.%E2%80%83

71、倪沪桅，钱骏．近红外二区荧光成像技术的临床研究进展［J］．红外与毫米波学报，2023，42（6）：896-906．

72、桂斌，姜楠，邓倾．近红外二区荧光成像在肿瘤诊疗中的研究进展［J］．中华核医学与分子影像杂志，2024，44（2）：120-123．

73、%E2%80%83%20LIN%E2%80%83E%EF%BC%8CSONG%E2%80%83M%EF%BC%8CWANG%E2%80%83B%EF%BC%8Cet%E2%80%83al%EF%BC%8EFibroblast%E2%80%83%0Aactivatio%20n%E2%80%83%20p%20rotei%20n%E2%80%83%20pe%20pti%20de-ta%20rgete%20d%E2%80%83%20N%20IR-%20I%2F%20I%20I%E2%80%83%0Afluorescence%E2%80%83imaging%E2%80%83for%E2%80%83stable%E2%80%83and%E2%80%83functional%E2%80%83detection%E2%80%83%0Aof%E2%80%83hepatocellular%E2%80%83carcinoma%EF%BC%BBJ%EF%BC%BD%EF%BC%8EEur%E2%80%83%20J%E2%80%83Nucl%E2%80%83Med%E2%80%83%0AMol%E2%80%83Imaging%EF%BC%8C2025%EF%BC%8C52%EF%BC%886%EF%BC%89%EF%BC%9A2157-2170%EF%BC%8E

1、广东省医学科研基金项目（A2024088）；广州市科技计划项目（2024A03J1016）；广州市中医药和中西医结合科技项目（20242A011001）()