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免疫炎症通过激活SOCS6/STAT6通路调控前列腺细胞增殖

SOCS6/STAT6 pathway regulates inflammatory proliferation of prostatic cells

来源期刊: 广州医药 | 1055-1060 发布时间:2025-09-17 收稿时间:2025/11/13 18:52:14 阅读量:28
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SOCS6STAT6前列腺增生炎症细胞增殖
SOCS6STAT6benign prostatic hyperplasiainflammationcell proliferation
DOI:
10.20223/j.cnki.1000-8535.2025.08.006
收稿时间:
2024-07-16 
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目的 研究SOCS6/STAT6通路在前列腺细胞炎性增殖作用中的调控作用。方法 使用人前列腺细胞株RWPE-1建立炎症模型,将细胞分为对照(CON)组和炎症刺激(INF)组,后者通过添加脂多糖(LPS)模拟炎症环境。采用ELISA检测白细胞介素-1β(IL-1β)-1β、白细胞介素-6(IL-6)和白细胞介素-8(IL-8)表达水平,蛋白免疫印迹法检测细胞因子信号抑制物-6(SOCS6)、信号转导和转录激活因子-6(STAT6)及磷酸化STAT6蛋白的表达水平。结果 经过LPS处理后,RWPE-1细胞中的SOCS6蛋白表达水平显著下降(P<0.01),而磷酸化STAT6表达水平上升(P<0.01)。结论 SOCS6/STAT6通路可能通过调节炎症环境下STAT6的磷酸化水平,参与调节前列腺细胞的炎性增殖作用。
Objective To explore the regulatory role of SOCS6/STAT6 pathway in the inflammatory proliferation of prostate cells.Methods The human prostate cell line RWPE-1 was used to establish an inflammation model.Cells were divided into a control(CON)group and an inflammation-stimulated(INF)group,with the latter subjected to lipopolysaccharide(LPS)treatment to simulate an inflammatory environment.The expression levels of interleukin(IL)-1β、IL-6 and IL-8 were detected by ELISA,and the expression levels of suppressor of cytokine signaling 6(SOCS6),signal transducer and activator oftranscription-6,(STAT6),and phosphorylated STAT6 proteins were detected by Western blot.Results The results showed that after LPS treatment,the expression of SOCS6 protein in RWPE-1 cells significantly decreased,while the expression of phosphorylated STAT6 increased.Conclusions The SOCS6/STAT6 pathway may be involved in regulating the inflammatory proliferation of prostate cells by modulating the phosphorylation level of STAT6 under inflammatory conditions.
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