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2023年7月 第38卷 第7期11
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子宫内膜异位症患者卵泡液外泌体miRNA谱差异及生信分析

Differential miRNA spectrum and bioinformatics analysis of follicular fluid exosomes in patients with endometriosis

来源期刊: 广州医药 | 324-330 发布时间:2024-04-12 收稿时间:2025/11/13 18:50:30 阅读量:10
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关键词:
子宫内膜异位症卵泡液外泌体微RNA卵母细胞
endometriosisfollicular fluidexosomemiRNAsoocyte
DOI:
10.3969/j.issn.1000-8535.2024.03.018
收稿时间:
2023-10-23 
修订日期:
 
接收日期:
 
引用总数:
3  
目的 探讨子宫内膜异位症(EMT)患者卵泡液来源的外泌体差异微小RNA(miRNA)对卵母细胞质量的影响。方法 收集2021年12月—2022年3月在广州市第一人民医院生殖医学中心进行体外受精-胚胎移植/卵细胞浆内单精子注射助孕的20例不孕症患者的卵泡液,分为EMT组(EMT不孕症患者10例)和对照组(单纯男性因素不孕症患者10例)。采用高通量测序对卵泡液外泌体微小RNA(miRNA)谱进行分析,选出具有组间差异的miRNAs。结果 与单纯男性因素不孕患者相比,EMT组有18个外泌体miRNAs差异有统计学意义,其中上调9个、下调9个。靶基因预测并采用GO和KEGG富集分析发现,这些靶基因主要参与磷脂酰肌醇-3-激酶/蛋白激酶B( PI3K-Akt)、核苷酸结合寡聚结构域NOD样受体、Ras等信号通路。结论 EMT患者卵泡液来源的外泌体miRNA存在差异,差异的外泌体miRNAs可能通过多个信号通路影响EMT患者卵母细胞质量。
Objective To investigate the effect of differential microRNA(miRNA)derived from follicular fluid exosomes on oocyte quality in patients with endometriosis(EMT). Methods Follicular fluid was collected from 20 infertile patients undergoing IVF-ET / ICSI in the Reproductive Medicine Center of Guangzhou First People's Hospital from December 2021 to March 2022,including EMT group(10 patients with EMT infertility)and control group(10 patients with simple male factor infertility).The miRNA spectrum in follicular fluid exosomes was analyzed by high-throughput sequencing and miRNAs with differences between groups were selected. Results Compared with patients with infertility due to simple male factors,there were significant differences in 18 exosomal miRNAs in the EMT group,of which 9 were up-regulated and 9 were down-regulated.GO and KEGG enrichment analysis showed that these target genes were mainly involved in phosphatidylinositol-3-kinase / protein kinase B,Nucleotide binding oligomerization domain-like receptor and other signaling pathways. Conclusions There are differences in follicular fluid-derived exosomal miRNAs in EMT patients.Differential exosomal miRNAs may affect oocyte quality in EMT patients through multiple signaling pathways.
1、 YANG D,LI N,MA A,et al.Identification of potential biomarkers of polycystic ovary syndrome via integrated bioinformatics analysis[J].Reprod Sci,2021,28(5):1353-1361. YANG D,LI N,MA A,et al.Identification of potential biomarkers of polycystic ovary syndrome via integrated bioinformatics analysis[J].Reprod Sci,2021,28(5):1353-1361.
2、 da LUZ C M,DA BROI M G,KOOPMAN L O,et al.Transcriptomic analysis of cumulus cells shows altered pathways in patients with minimal and mild endometriosis[J].Sci Rep,2022,12(1):5775. da LUZ C M,DA BROI M G,KOOPMAN L O,et al.Transcriptomic analysis of cumulus cells shows altered pathways in patients with minimal and mild endometriosis[J].Sci Rep,2022,12(1):5775.
3、 ANDRADE G M,da SILVEIRA J C,PERRINI C,et al.The role of the PI3K-Akt signaling pathway in the developmental competence of bovine oocytes[J].PLoS One,2017,12(9):e0185045. ANDRADE G M,da SILVEIRA J C,PERRINI C,et al.The role of the PI3K-Akt signaling pathway in the developmental competence of bovine oocytes[J].PLoS One,2017,12(9):e0185045.
4、 ANDREAS E,HOELKER M,NEUHOFF C,et al.microRNA 17-92 cluster regulates proliferation and differentiation of bovine granulosa cells by targeting PTEN and BMPR2 genes[J].Cell Tissue Res,2016,366(1):219-230. ANDREAS E,HOELKER M,NEUHOFF C,et al.microRNA 17-92 cluster regulates proliferation and differentiation of bovine granulosa cells by targeting PTEN and BMPR2 genes[J].Cell Tissue Res,2016,366(1):219-230.
5、 ARTINI P G,TATONE C,SPERDUTI S,et al.Cumulus cells surrounding oocytes with high developmental competence exhibit down regulation of phosphoinositol 1,3 kinase/protein kinase B(PI3K/AKT)signalling genes involved in proliferation and survival[J].Hum Reprod,2017,32(12):2474-2484. ARTINI P G,TATONE C,SPERDUTI S,et al.Cumulus cells surrounding oocytes with high developmental competence exhibit down regulation of phosphoinositol 1,3 kinase/protein kinase B(PI3K/AKT)signalling genes involved in proliferation and survival[J].Hum Reprod,2017,32(12):2474-2484.
6、 NOTHNICK W B,SWAN K,FLYCKT R,et al.Human endometriotic lesion expression of the miR-144-3p/miR-451a cluster,its correlation with markers of cell survival and origin of lesion content[J].Sci Rep,2019,9(1):8823. NOTHNICK W B,SWAN K,FLYCKT R,et al.Human endometriotic lesion expression of the miR-144-3p/miR-451a cluster,its correlation with markers of cell survival and origin of lesion content[J].Sci Rep,2019,9(1):8823.
7、 沈开元,黄芬,罗江霞,等.卵巢功能减退患者卵泡液外泌体miRNA表达谱分析研究[J].生殖医学杂志,2021,30(1):99-106. 沈开元,黄芬,罗江霞,等.卵巢功能减退患者卵泡液外泌体miRNA表达谱分析研究[J].生殖医学杂志,2021,30(1):99-106.
8、 温惠慧,冯云,张丽娜,等.PCOS患者卵泡液外泌体miRNA谱差异表达及生物信息学分析[J].生殖医学杂志,2021,30(8):1067-1075. 温惠慧,冯云,张丽娜,等.PCOS患者卵泡液外泌体miRNA谱差异表达及生物信息学分析[J].生殖医学杂志,2021,30(8):1067-1075.
9、 胡頲,胡蓉,倪亚莉等.卵泡微环境中外泌体对体外受精-胚胎移植妊娠结局的影响[J].中华生殖与避孕杂志,2020,40(2):115-123. 胡頲,胡蓉,倪亚莉等.卵泡微环境中外泌体对体外受精-胚胎移植妊娠结局的影响[J].中华生殖与避孕杂志,2020,40(2):115-123.
10、 胡頲,胡蓉,倪亚莉,等.卵泡微环境中外泌体对体外受精-胚胎移植妊娠结局的影响[J].中华生殖与避孕杂志,2020,40(2):115-123. 胡頲,胡蓉,倪亚莉,等.卵泡微环境中外泌体对体外受精-胚胎移植妊娠结局的影响[J].中华生殖与避孕杂志,2020,40(2):115-123.
11、 ZHANG A,WANG G,JIA L,et al.Exosome-mediated microRNA-138 and vascular endothelial growth factor in endometriosis through in?ammation and apoptosis via the nuclear factor-κB signaling pathway[J].Int J Mol Med,2019,43(1):358-370. ZHANG A,WANG G,JIA L,et al.Exosome-mediated microRNA-138 and vascular endothelial growth factor in endometriosis through in?ammation and apoptosis via the nuclear factor-κB signaling pathway[J].Int J Mol Med,2019,43(1):358-370.
12、 JIANG Y,CHAI X,CHEN S,et al.Exosomes from the uterine cavity mediate immune dysregulation via inhibiting the JNK signal pathway in endometriosis[J].Biomedicines,2022,10(12):3110. JIANG Y,CHAI X,CHEN S,et al.Exosomes from the uterine cavity mediate immune dysregulation via inhibiting the JNK signal pathway in endometriosis[J].Biomedicines,2022,10(12):3110.
13、 DU J,SHEN J,WANG Y,et al.Boar seminal plasma exosomes maintain sperm function by infiltrating into the sperm membrane[J].Oncotarget,2016,7(37):58832-58847. DU J,SHEN J,WANG Y,et al.Boar seminal plasma exosomes maintain sperm function by infiltrating into the sperm membrane[J].Oncotarget,2016,7(37):58832-58847.
14、 da SILVEIRA J C,de áVILA ACFCM,GARRETT H L,et al.Cell-secreted vesicles containing microRNAs as regulators of gamete maturation[J].Endocrinol,2018,236(1):R15-R27. da SILVEIRA J C,de áVILA ACFCM,GARRETT H L,et al.Cell-secreted vesicles containing microRNAs as regulators of gamete maturation[J].Endocrinol,2018,236(1):R15-R27.
15、 LOBB R J,LIMA L G,MOLLER A.Exosomes:Key mediators of metastasis and pre-metastatic niche formation[J].Semin Cell Dev Biol,2017(67):3-10. LOBB R J,LIMA L G,MOLLER A.Exosomes:Key mediators of metastasis and pre-metastatic niche formation[J].Semin Cell Dev Biol,2017(67):3-10.
16、 NAWAZ M,SHAH N,ZANETTI B R,et al.Extracellular vesicles and matrix remodeling enzymes:The emerging roles in extracellular matrix remodeling,progression of diseases and tissue repair[J]Cells,2018,7(10):167. NAWAZ M,SHAH N,ZANETTI B R,et al.Extracellular vesicles and matrix remodeling enzymes:The emerging roles in extracellular matrix remodeling,progression of diseases and tissue repair[J]Cells,2018,7(10):167.
17、 IRANIFAR E,SERESHT B M,MOMENI F,et al.Exosomes and microRNAs:New potential therapeutic candidates in Alzheimer disease therapy[J].J Cell Physiol,2019,234(3):2296-2305. IRANIFAR E,SERESHT B M,MOMENI F,et al.Exosomes and microRNAs:New potential therapeutic candidates in Alzheimer disease therapy[J].J Cell Physiol,2019,234(3):2296-2305.
18、 ZHANG W,JIANG X,BAO J,et al.Exosomes in pathogen infections:a bridge to deliver molecules and link functions[J].Front Immunol,2018(9):90. ZHANG W,JIANG X,BAO J,et al.Exosomes in pathogen infections:a bridge to deliver molecules and link functions[J].Front Immunol,2018(9):90.
19、 谢幸,孔北华,段涛.妇产科学[M].9版.北京:人民卫生出版社,2019:361-363. 谢幸,孔北华,段涛.妇产科学[M].9版.北京:人民卫生出版社,2019:361-363.
20、 杨倩,刘兰心,黄荷凤.多囊卵巢综合征患者卵泡液外泌体的提取鉴定及其 miRNAs 的提取和检测[J].上海交通大学学报(医学版),2017,37(8):1085-1089. 杨倩,刘兰心,黄荷凤.多囊卵巢综合征患者卵泡液外泌体的提取鉴定及其 miRNAs 的提取和检测[J].上海交通大学学报(医学版),2017,37(8):1085-1089.
21、 PLA I,SANCHEZ A,PORS S E,et al.Proteome of fluid from human ovarian small antral follicles reveals insights in folliculogenesis and oocyte maturation[J].Hum Reprod,2021,36(3):756-770. PLA I,SANCHEZ A,PORS S E,et al.Proteome of fluid from human ovarian small antral follicles reveals insights in folliculogenesis and oocyte maturation[J].Hum Reprod,2021,36(3):756-770.
22、 MUTESHI C M,OHUMA E O,CHILD T,et al.The effect of endometriosis on live birth rate and other reproductive outcomes in ART cycles:A cohort study[J].Hum Reprod Open,2018,2018(4):hoy016. MUTESHI C M,OHUMA E O,CHILD T,et al.The effect of endometriosis on live birth rate and other reproductive outcomes in ART cycles:A cohort study[J].Hum Reprod Open,2018,2018(4):hoy016.
23、 中国医师协会妇产科医师分会,中华医学会妇产科学分会子宫内膜异位症协作组.子宫内膜异位症诊治指南(第三版)[J].中华妇产科杂志,2021,56(12):812-824. 中国医师协会妇产科医师分会,中华医学会妇产科学分会子宫内膜异位症协作组.子宫内膜异位症诊治指南(第三版)[J].中华妇产科杂志,2021,56(12):812-824.
1、林桂娇,周金宵,栾毅峰,等.miRNA与子宫内膜异位症不孕关系的研究进展[J].广西医学,2024,46(09):1423-1427. 林桂娇,周金宵,栾毅峰,等.miRNA与子宫内膜异位症不孕关系的研究进展[J].广西医学,2024,46(09):1423-1427.
2、黄素珍,陈燕萍,倪志翔.对比超声和磁共振成像诊断子宫内膜异位症中的临床价值研究[J].中国医疗器械信息,2024,30(20):27-29+113.DOI:10.15971/j.cnki.cmdi.2024.20.025. 黄素珍,陈燕萍,倪志翔.对比超声和磁共振成像诊断子宫内膜异位症中的临床价值研究[J].中国医疗器械信息,2024,30(20):27-29+113.DOI:10.15971/j.cnki.cmdi.2024.20.025.
3、严兆华,方醒艺,姚美华,等.外泌体和免疫细胞在子宫内膜异位症发生与发展中的作用研究进展[J].陕西医学杂志,2025,54(02):285-288. 严兆华,方醒艺,姚美华,等.外泌体和免疫细胞在子宫内膜异位症发生与发展中的作用研究进展[J].陕西医学杂志,2025,54(02):285-288.
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