基因芯片初步探讨乳腺癌转移相关蛋白Nucleobindin-2介导的信号通路

来源期刊：广州医药 | 56-67 发布时间：2022-04-12 收稿时间：2025/11/13 18:24:08 阅读量：24

作者：: 符青云,

欧阳小明,

郅程,

曾亮,

关键词：: 乳腺癌通路基因芯片核结合蛋白2; breast cancer pathway gene chip nucleobindin-2

DOI：: 10.3969/j.issn.1000-8535.2022.02.013

收稿时间：: 2021-09-23
修订日期：
接收日期：
引用总数：: 0

目的探讨核结合蛋白2(NUCB2)介导的下游信号分子和通路,为阐明NUCB2在乳腺癌中的功能提供依据。方法构建NUCB2-RNAi慢病毒载体,感染MDA-MB-231细胞株。然后将MDA-MB-231分为阴性对照病毒感染细胞组(NC组)、感染NUCB2基因shRNA病毒细胞组(KD组),用Affymetrix基因表达谱芯片对NUCB2下游基因进行筛选,并对所有数据进行独创性通路分析(IPA)分析。用qPCR测定mRNA水平。统计采用SPSS 20.0软件。结果 Path-Array研究筛选了KD组与NC组的差异基因,其中上调基因186个,下调基因356个,部分差异表达基因的检测表明,这些基因的mRNA水平与Path-Array筛选结果一致。IPA分析显示,经典途径中差异表达基因的显著富集表明胆固醇生物合成的超途径被显著抑制。上游调节因子分析显示了所有不同表达基因的上游调节因子,包括转录因子、细胞因子、小RNA、受体、激酶、化学分子和药物。疾病和功能差异表达基因的显著丰富表明,与NUCB2相关的差异表达基因与41种疾病和功能显著相关,更多与癌症、组织损伤和异常相关。结论 NUCB2的功能涉及多种基因和多种信号通路。

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