您的位置: 首页 > 2022年9月 第52卷 第5期 > 文字全文
2023年7月 第38卷 第7期11
目录

青藤碱对肾癌细胞786-O细胞增殖、细胞周期及凋亡的影响

Effects of sinomenine on cell cycle and apoptosis of 786-O renal carcinoma cells

来源期刊: 广州医药 | 38-42 发布时间:2022-10-11 收稿时间:2025/11/13 18:17:29 阅读量:26
作者:
关键词:
肾癌青藤碱增殖细胞周期凋亡
renal cancersinomenineproliferationcell cycleapoptosis
DOI:
10.3969/j.issn.1000-8535.2022.05.007
收稿时间:
2022-01-15 
修订日期:
 
接收日期:
 
引用总数:
5  
目的 探讨不同浓度的青藤碱对肾癌细胞增殖、细胞周期及凋亡的影响。方法 以不同浓度的青藤碱处理肾癌细胞786-O,采用四氮唑蓝盐法检测细胞增殖能力,流式细胞术检测细胞周期分布,Annexin-v FITC/PI双染流式细胞分析仪检测细胞凋亡率;采用实时荧光定量PCR及蛋白免疫印迹(WB)检测c-myc、Bax、Caspase-3等细胞周期、凋亡相关基因表达情况。结果 青藤碱显著抑制786-O细胞的增殖能力,诱导细胞周期G1/S期阻滞及细胞凋亡;且随着青藤碱浓度的增加,其抑制率也逐渐增加;青藤碱显著下调c-myc蛋白表达,而诱导凋亡蛋白Bax、Caspase-3表达上调。结论 青藤碱可以显著抑制786-O细胞中c-myc表达,使其增殖能力减弱,诱导细胞周期阻滞及凋亡。青藤碱可能具有潜在的抑制肾癌生长作用。
Objective To investigate the effects of different concentrations of sinomenine on proliferation, cell cycle and apoptosis of renal carcinoma cells. Methods The renal carcinoma cells were treated with different concentrations of sinomenine. MTS was used to analyze the effects of sinomenine on proliferation in 786-O renal carcinoma cells, the cell cycle changes were determined using flow cytometry, while the changes of apoptosis were detected by Annexin V-FITC / PI double staining. The expression of apoptosis-related proteins such as c-myc, Bax and Caspase-3 were detected by Western blot. Results Sinomenine significantly inhibited the proliferation of 786-O cells and induced cell cycle arrest and apoptosis in G1/S phase. With the increase of sinomenine concentration, the inhibition rate increased gradually. Sinomenine significantly down-regulated the expression of c-myc protein, while the expressions of the apoptotic protein Bax, Caspase-3 were up-regulated. Conclusions Sinomenine can significantly inhibit the expression of c-myc in 786-O cells, reduce proliferation ability, and induce cell cycle arrest and apoptosis. Sinomenine may have a potential therapeutic effect on renal cancer.
1、 ZHOU L, LUAN H, LIU Q, et al. Activation of PI3K/Akt and ERK signaling pathways antagonized sinomenine-induced lung cancer cell apoptosis [J]. Mol Med Rep, 2012, 5(5): 1256-1260. ZHOU L, LUAN H, LIU Q, et al. Activation of PI3K/Akt and ERK signaling pathways antagonized sinomenine-induced lung cancer cell apoptosis [J]. Mol Med Rep, 2012, 5(5): 1256-1260.
2、 LIU Z, DUAN Z J, CHANG J Y, et al. Sinomenine sensitizes multidrug-resistant colon cancer cells (Caco-2) to doxorubicin by downregulation of MDR-1 expression [J]. PLoS One, 2014, 9(6): e98560. LIU Z, DUAN Z J, CHANG J Y, et al. Sinomenine sensitizes multidrug-resistant colon cancer cells (Caco-2) to doxorubicin by downregulation of MDR-1 expression [J]. PLoS One, 2014, 9(6): e98560.
3、 QU X, YU B, ZHU M, et al. Sinomenine inhibits the growth of ovarian cancer cells through the suppression of mitosis by down-regulating the expression and the activity of CDK1 [J]. Onco Targets Ther, 2021(14): 823-834. QU X, YU B, ZHU M, et al. Sinomenine inhibits the growth of ovarian cancer cells through the suppression of mitosis by down-regulating the expression and the activity of CDK1 [J]. Onco Targets Ther, 2021(14): 823-834.
4、 LI X, LI P, LIU C, et al. Sinomenine hydrochloride inhibits breast cancer metastasis by attenuating inflammation-related epithelial-mesenchymal transition and cancer stemness [J]. Oncotarget, 2017, 8(8): 13560-13574. LI X, LI P, LIU C, et al. Sinomenine hydrochloride inhibits breast cancer metastasis by attenuating inflammation-related epithelial-mesenchymal transition and cancer stemness [J]. Oncotarget, 2017, 8(8): 13560-13574.
5、 LIU W, ZHANG Y, ZHU W, et al. Sinomenine inhibits the progression of rheumatoid arthritis by regulating the secretion of inflammatory cytokines and monocyte/macrophage subsets [J]. Front Immunol, 2018(9): 2228. LIU W, ZHANG Y, ZHU W, et al. Sinomenine inhibits the progression of rheumatoid arthritis by regulating the secretion of inflammatory cytokines and monocyte/macrophage subsets [J]. Front Immunol, 2018(9): 2228.
6、 LEE J Y, KIM K J, KIM J, et al. Anti-osteoclastogenic effects of isoquinoline alkaloids from the rhizome extract of Sinomenium acutum [J]. Arch Pharm Res, 2016, 39(5): 713-20. LEE J Y, KIM K J, KIM J, et al. Anti-osteoclastogenic effects of isoquinoline alkaloids from the rhizome extract of Sinomenium acutum [J]. Arch Pharm Res, 2016, 39(5): 713-20.
7、 陆淼炯,褚卫建. 魔芋葡甘露聚糖逆转大肠癌5-氟尿嘧啶耐药的作用及其机制研究[J]. 中国现代应用药学,2021,38(10):1188-1194. 陆淼炯,褚卫建. 魔芋葡甘露聚糖逆转大肠癌5-氟尿嘧啶耐药的作用及其机制研究[J]. 中国现代应用药学,2021,38(10):1188-1194.
8、 鹿蓓蓓,傅鹤秀,ERIC ACHIBOREBADOR OKRAH 等,穿心莲内酯对小鼠乳腺癌的抑制作用和机制[J]. 中国药理学通报,2022,38(1):26-32. 鹿蓓蓓,傅鹤秀,ERIC ACHIBOREBADOR OKRAH 等,穿心莲内酯对小鼠乳腺癌的抑制作用和机制[J]. 中国药理学通报,2022,38(1):26-32.
9、 RATHMELL W K, GODLEY P A. Recent updates in renal cell carcinoma [J]. Curr Opin Oncol,2010, 22(3):250-256. RATHMELL W K, GODLEY P A. Recent updates in renal cell carcinoma [J]. Curr Opin Oncol,2010, 22(3):250-256.
10、 LJUNGBERG B, CAMPBELL S C, CHOI H Y, et al. The epidemiology of renal cell carcinoma [J]. Eur Urol, 2011, 60(4):615-621. LJUNGBERG B, CAMPBELL S C, CHOI H Y, et al. The epidemiology of renal cell carcinoma [J]. Eur Urol, 2011, 60(4):615-621.
11、 LINEHAN W M. Genetic basis of kidney cancer: role of genomics for the development of disease-based therapeutics [J]. Genome Res,2012, 22(11):2089-100. LINEHAN W M. Genetic basis of kidney cancer: role of genomics for the development of disease-based therapeutics [J]. Genome Res,2012, 22(11):2089-100.
12、 MAURANO M T, RICHARD H, ERIC R, et al. Systematic localization of common disease-associated variation in regulatory DNA [J]. Science, 2012, 337(6099):1190-1195. MAURANO M T, RICHARD H, ERIC R, et al. Systematic localization of common disease-associated variation in regulatory DNA [J]. Science, 2012, 337(6099):1190-1195.
13、 LV Y, LI C, LI S, et al. Sinomenine inhibits proliferation of SGC-7901 gastric adenocarcinoma cells via suppression of cyclooxygenase-2 expression [J]. Oncol Lett, 2011, 2(4): 741-745. LV Y, LI C, LI S, et al. Sinomenine inhibits proliferation of SGC-7901 gastric adenocarcinoma cells via suppression of cyclooxygenase-2 expression [J]. Oncol Lett, 2011, 2(4): 741-745.
14、 王昊,刘天盛,陈旭义. 青藤碱对胶原诱导性关节炎大鼠治疗作用 [J]. 中华实用诊断与治疗杂志, 2015, 29(6): 535-537. 王昊,刘天盛,陈旭义. 青藤碱对胶原诱导性关节炎大鼠治疗作用 [J]. 中华实用诊断与治疗杂志, 2015, 29(6): 535-537.
15、 罗进芳,朱易浪,董燕,等.青藤碱对LPS、IL-4诱导的小鼠RAW264.7巨噬细胞极化的影响 [J]. 中国免疫学杂志, 2015, 31(1):56-60. 罗进芳,朱易浪,董燕,等.青藤碱对LPS、IL-4诱导的小鼠RAW264.7巨噬细胞极化的影响 [J]. 中国免疫学杂志, 2015, 31(1):56-60.
16、 ZHU Q, SUN Y, MAO L, et al. Antinociceptive effects of sinomenine in a rat model of postoperative pain [J]. Br J Pharmacol, 2016, 173(10): 1693-1702. ZHU Q, SUN Y, MAO L, et al. Antinociceptive effects of sinomenine in a rat model of postoperative pain [J]. Br J Pharmacol, 2016, 173(10): 1693-1702.
17、 LIU S, MAN Y, ZHAO L. Sinomenine inhibits lipopolysaccharide-induced inflammatory injury by regulation of miR-101/MKP-1/JNK pathway in keratinocyte cells [J]. Biomed Pharmacother, 2018(101): 422-429. LIU S, MAN Y, ZHAO L. Sinomenine inhibits lipopolysaccharide-induced inflammatory injury by regulation of miR-101/MKP-1/JNK pathway in keratinocyte cells [J]. Biomed Pharmacother, 2018(101): 422-429.
18、 ZHAO M M, YANG B, ZHANG Q, et al. Sinomenine hydrochloride attenuates renal fibrosis by inhibiting excessive autophagy induced by adriamycin: an experimental study [J]. Evid Based Complement Alternat Med, 2017(2017): 6878795. ZHAO M M, YANG B, ZHANG Q, et al. Sinomenine hydrochloride attenuates renal fibrosis by inhibiting excessive autophagy induced by adriamycin: an experimental study [J]. Evid Based Complement Alternat Med, 2017(2017): 6878795.
19、 张洪长, 刘明昕, 张莹,等.青藤碱对类风湿关节炎成纤维样滑膜细胞MyD88、TRAF-6表达的影响 [J]. 中国免疫学杂志, 2015(4): 485-489. 张洪长, 刘明昕, 张莹,等.青藤碱对类风湿关节炎成纤维样滑膜细胞MyD88、TRAF-6表达的影响 [J]. 中国免疫学杂志, 2015(4): 485-489.
20、 YAMASAKI H. Pharmacology of sinomenine, an anti-rheumatic alkaloid from Sinomenium acutum [J]. Acta Med Okayama, 1976, 30(1): 1-20. YAMASAKI H. Pharmacology of sinomenine, an anti-rheumatic alkaloid from Sinomenium acutum [J]. Acta Med Okayama, 1976, 30(1): 1-20.
1、罗燕仙.青风藤雌雄株青藤碱含量及功能差异分析[D].中南林业科技大学,2025.DOI:10.27662/d.cnki.gznlc.2025.000548. 罗燕仙.青风藤雌雄株青藤碱含量及功能差异分析[D].中南林业科技大学,2025.DOI:10.27662/d.cnki.gznlc.2025.000548.
2、张广丽,黎壮伟,宋卉.龟板固本方对肺癌骨髓抑制小鼠骨髓细胞周期及凋亡相关蛋白表达的影响[J].现代中西医结合杂志,2023,32(16):2249-2252. 张广丽,黎壮伟,宋卉.龟板固本方对肺癌骨髓抑制小鼠骨髓细胞周期及凋亡相关蛋白表达的影响[J].现代中西医结合杂志,2023,32(16):2249-2252.
3、贾梦雪,朱静平,王振兴,等.青风藤化学成分、药理和临床应用研究进展[J].中华中医药学刊,2025,43(02):187-193+294.DOI:10.13193/j.issn.1673-7717.2025.02.039. 贾梦雪,朱静平,王振兴,等.青风藤化学成分、药理和临床应用研究进展[J].中华中医药学刊,2025,43(02):187-193+294.DOI:10.13193/j.issn.1673-7717.2025.02.039.
4、王志丹,张微.青藤碱调控COX-2/VEGF通路抑制肝癌HepG2细胞增殖和侵袭[J].解剖科学进展,2024,30(04):429-431+435.DOI:10.16695/j.cnki.1006-2947.2024.04.025. 王志丹,张微.青藤碱调控COX-2/VEGF通路抑制肝癌HepG2细胞增殖和侵袭[J].解剖科学进展,2024,30(04):429-431+435.DOI:10.16695/j.cnki.1006-2947.2024.04.025.
5、胡伊凌,张文戈,陈远帆,等.癌症患者生命质量测定量表体系之肾癌量表(QLICP-REV2.0)的条目筛选[J].广州医药,2025,56(01):77-81.DOI:10.20223/j.cnki.1000-8535.2025.01.011. 胡伊凌,张文戈,陈远帆,等.癌症患者生命质量测定量表体系之肾癌量表(QLICP-REV2.0)的条目筛选[J].广州医药,2025,56(01):77-81.DOI:10.20223/j.cnki.1000-8535.2025.01.011.
上一篇
下一篇
出版者信息








《广州医药》公众号
目录