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乳腺癌关键基因的生物信息学分析

Bioinformatic analysis of key genes in breast cancer

来源期刊: 广州医药 | 65-71 发布时间:2021-11-22 收稿时间:2025/11/13 18:13:23 阅读量:23
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关键词:
乳腺癌差异基因生物信息学生存率
Breast cancerDifferentially expressed genesBioinformaticsSurvival
DOI:
10.3969/j.issn.1000-8535.2021.01.013
收稿时间:
2020-06-18 
修订日期:
 
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引用总数:
0  
目的 乳腺癌是世界范围内最常见的恶性肿瘤之一。目前,人们对乳腺癌的发病机制进行了大量的研究,但对其分子机制的认识尚不清楚。本研究采用生物信息学技术,筛选乳腺癌潜在的关键基因,最终为乳腺癌的诊断、治疗及预后判断提供潜在的生物标记物。方法 从基因表达综合数据库(GEO)下载基因芯片GSE36295、GSE71053和GSE86374,通过GEO2R鉴定差异表达基因(DEGs),并进行功能富集分析。利用STRING构建了蛋白质-蛋白质相互作用网络(PPI),并采用Cytoscape进行了模块分析。结果 共鉴定出95个DEGs,包括62个上调基因和33个下调基因。共鉴定出10个Hub基因:CENPF、KIF2C、TOP2A、NUSAP1、HMMR、MELK、KIF4A、ASPM、CEP55、CCNB1。结论 本研究发现的Hub基因可能对乳腺癌的发展和预后存在一定影响,为乳腺癌的诊断和治疗提供候选靶点。
Objective Breast cancer is one of the most common cancers worldwide. At present, a lot of researches have been carried out on the pathogenesis of breast cancer, but the molecular mechanisms of breast cancer are still not well understood. In this study, bioinformatics technology was used to screen the potential key genes of breast cancer, and ultimately to provide potential biomarkers for the diagnosis, treatment and prognosis of breast cancer. Methods The microarray datasets GSE36295、GSE71053和GSE86374 were downloaded from Gene Expression Omnibus (GEO), and the differentially expressed genes (DEGs) were identified by GEO2R, and the enriched functions and pathways of the DEGs were analyzed. Protein-protein interaction network (PPI) was constructed by using String, and the module analysis was performed using Cytoscape. Results A total of 95 DEGs were identified, consisting of 62 upregulated genes and 33 downregulated genes.Ten hub genes were identified: CENPF,KIF2C,TOP2A,NUSAP1,HMMR,MELK,KIF4A,ASPM,CEP55,CCNB1. Conclusion The hub gene was found in this study may be involved in the development and prognosis of breast cancer. It may provide candidate targets for diagnosis and treatment of breast cancer.
1、 SONG Y, ZHAO C, DONG L, et al. Overexpression of cyclin B1 in human esophagealsquamous cell carcinoma cells induces tumor cell invasive growth andmetastasis[J]. Carcinogenesis, 2008, 29(2):307-315. SONG Y, ZHAO C, DONG L, et al. Overexpression of cyclin B1 in human esophagealsquamous cell carcinoma cells induces tumor cell invasive growth andmetastasis[J]. Carcinogenesis, 2008, 29(2):307-315.
2、 JEFFERY J, SINHA D, SRIHARI S, et al. Beyond cytokinesis:the emerging roles of CEP55 in tumorigenesis[J]. Oncogene, 2016,35(6):683-690. JEFFERY J, SINHA D, SRIHARI S, et al. Beyond cytokinesis:the emerging roles of CEP55 in tumorigenesis[J]. Oncogene, 2016,35(6):683-690.
3、 XUE D, CHENG P, HAN M J, et al. An Integrated bioinformatical analysis to evaluate the role of KIF4A as a prognostic biomarker for breast cancer [J]. Onco Targets Ther, 2018,10(11):4755-4768. XUE D, CHENG P, HAN M J, et al. An Integrated bioinformatical analysis to evaluate the role of KIF4A as a prognostic biomarker for breast cancer [J]. Onco Targets Ther, 2018,10(11):4755-4768.
4、 CHRISTOPHER J G, ANN L, JOAN C S, et al. MELK expression correlates with tumormitotic activity but is not required forcancer growth[J]. ELife, 2018,8(7):e32838. CHRISTOPHER J G, ANN L, JOAN C S, et al. MELK expression correlates with tumormitotic activity but is not required forcancer growth[J]. ELife, 2018,8(7):e32838.
5、 ZHANG H Z, REN L L, DING Y, et al. Hyaluronan-mediated motility receptor confers resistance to chemotherapy via TGFβ/Smad2-induced epithelial-mesenchymal transition in gastric cancer[J]. ASEB J, 2019,33(5):6365-6377. ZHANG H Z, REN L L, DING Y, et al. Hyaluronan-mediated motility receptor confers resistance to chemotherapy via TGFβ/Smad2-induced epithelial-mesenchymal transition in gastric cancer[J]. ASEB J, 2019,33(5):6365-6377.
6、 ZHANG X, PAN Y L,FU H Q, et al. Nucleolar and spindle associated protein 1 (nusap1) inhibits cell proliferation and enhances susceptibility to epirubicin in invasive breast cancer cells by regulating Cyclin D Kinase (CDK1) and DLGAP5 expression[J]. Med Sci Monit, 2018,26(24):8553-8564. ZHANG X, PAN Y L,FU H Q, et al. Nucleolar and spindle associated protein 1 (nusap1) inhibits cell proliferation and enhances susceptibility to epirubicin in invasive breast cancer cells by regulating Cyclin D Kinase (CDK1) and DLGAP5 expression[J]. Med Sci Monit, 2018,26(24):8553-8564.
7、 ENGSTR M J, YTTERHUS B, VATTEN L J, et al. TOP2A gene copy numberchange in breast cancer[J]. J Clin Pathol, 2014, 67(5):420-425. ENGSTR M J, YTTERHUS B, VATTEN L J, et al. TOP2A gene copy numberchange in breast cancer[J]. J Clin Pathol, 2014, 67(5):420-425.
8、 WANG C Q, XIANG F G, LI Y J, et al. Relation between the expression of mitotic centromere-associated kinesin and the progression ofsquamous cell carcinoma of the tongue[J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2014,117(3):353-360. WANG C Q, XIANG F G, LI Y J, et al. Relation between the expression of mitotic centromere-associated kinesin and the progression ofsquamous cell carcinoma of the tongue[J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2014,117(3):353-360.
9、 ZHANG L Y, SHAO H Y, HUANG Y J, et al. PLK1 phosphorylates mitotic centromere-associated kinesin and promotes its depolymerase activity[J]. J Biol Chem, 2010,286(4):3033-3046. ZHANG L Y, SHAO H Y, HUANG Y J, et al. PLK1 phosphorylates mitotic centromere-associated kinesin and promotes its depolymerase activity[J]. J Biol Chem, 2010,286(4):3033-3046.
10、 SUN B, LIN G, JI D, et al. Dysfunction of sister chromatids separation promotes progression of hepatocellular carcinoma according to analysis of gene expression profiling[J]. Front Physiol, 2018,27(9):1019. SUN B, LIN G, JI D, et al. Dysfunction of sister chromatids separation promotes progression of hepatocellular carcinoma according to analysis of gene expression profiling[J]. Front Physiol, 2018,27(9):1019.
11、 WANG Y, CHENG J, XU C, et al. Quantitative methylation analysis reveals gender and age differences in p16INK4a hypermethylation in hepatitis B virus-related hepatocellular carcinoma[J]. Liver Int, 2012,32(3):420-428. WANG Y, CHENG J, XU C, et al. Quantitative methylation analysis reveals gender and age differences in p16INK4a hypermethylation in hepatitis B virus-related hepatocellular carcinoma[J]. Liver Int, 2012,32(3):420-428.
12、 CHEN W, ZHENG R, BAADE P D, et al. Cancer statistics in China,2015[J]. CA Cancer J Clin, 2016,66(2):115-132. CHEN W, ZHENG R, BAADE P D, et al. Cancer statistics in China,2015[J]. CA Cancer J Clin, 2016,66(2):115-132.
13、 FREDDIE B, JACQUES F, ISABELLE S, et al. Global cancer statistics 2018: globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2018,68(6):394-424. FREDDIE B, JACQUES F, ISABELLE S, et al. Global cancer statistics 2018: globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2018,68(6):394-424.
14、 MA X J, SONIKA D, ELIZABETH R, et al. Gene expression profiling of the tumor microenvironment during breast cancer progression[J]. Breast Cancer Res, 2009,11(1):R7. MA X J, SONIKA D, ELIZABETH R, et al. Gene expression profiling of the tumor microenvironment during breast cancer progression[J]. Breast Cancer Res, 2009,11(1):R7.
15、 CHRISTINA C, SOHRAB P S, SUET-FEUNG C, et al. The Genomic and transcriptomic architecture of 2 000 breast tumours reveals novel subgroups[J]. Nature, 2012,486(7403):346-352. CHRISTINA C, SOHRAB P S, SUET-FEUNG C, et al. The Genomic and transcriptomic architecture of 2 000 breast tumours reveals novel subgroups[J]. Nature, 2012,486(7403):346-352.
16、 LONG T, LIU Z, ZHOU X, et al. Identification of differentially expressed genes and enriched pathways in lung cancer using bioinformatics analysis[J]. Mol Med Rep, 2019,19(3):2029-2040. LONG T, LIU Z, ZHOU X, et al. Identification of differentially expressed genes and enriched pathways in lung cancer using bioinformatics analysis[J]. Mol Med Rep, 2019,19(3):2029-2040.
17、 BRETTHAUER M, KALAGER M. Principles, effectiveness and caveats in screening for cancer[J]. Br J Surg, 2013,100(1):55-65. BRETTHAUER M, KALAGER M. Principles, effectiveness and caveats in screening for cancer[J]. Br J Surg, 2013,100(1):55-65.
18、 FEI W. Current status of breast cancer prevention in China[J]. Chron Dis Transl Med,2015,1(1):2-8. FEI W. Current status of breast cancer prevention in China[J]. Chron Dis Transl Med,2015,1(1):2-8.
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