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细胞间质液压升高对肝星状细胞的影响

Influence of interstitial cell fluid pressure rising to hepatic stellate cell

来源期刊: 广州医药 | 130-134 发布时间:2021-11-28 收稿时间:2025/11/13 17:41:25 阅读量:34
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关键词:
肝星状细胞细胞间质液压RhoA/ROCK1信号通路
DOI:
10.3969/j.issn.1000-8535.2020.05.030
收稿时间:
2020-03-15 
修订日期:
 
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引用总数:
0  
目的 从生物力学应力调控角度,探讨组织间质液压升高诱发肝纤维化的分子细胞机制。方法 人肝星状细胞随机分为3组,模型组:将其置于压力箱中施加恒定的高于大气压50 mmHg压力;实验组:加入ROCK抑制剂Y-27632(浓度10 μmol)置于与实验组相同条件;对照组:不加压置于相同培养箱中。应用RC-PCR检测其α-SMA、RhoA、ROCK mRNA的表达量,并应用免疫荧光染色分析其α-SMA、ROCK1、ROCK2蛋白表达情况。结果 间质液压变化对人肝星状细胞中α-SMAmRNA的表达量比对照组表达量增加,RhoA mRNA的表达先上升后下降,只对早期压力有变化,而 ROCK mRNA的表达量无明显变化。抑制剂组和压力组α-SMA 、ROCK1荧光强度较对照组增强,ROCK2荧光强度无明显变化,其中模型变化更显著。结论 细胞间质液压升高能通过RhoA/ROCK1信号通路引起肝星状细胞的活化。
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