您的位置: 首页 > 2018年11月 第49卷 第6期 > 文字全文
2023年7月 第38卷 第7期11
目录

Wnt-1信号通路蛋白-3(WISP-3)在高氧诱导肺上皮细胞凋亡中的保护作用

The protective effect of Wnt-1 inducible signaling pathway protein-3 (WISP-3) in hyperoxia-induced apoptosis in lung epithelial Cells

来源期刊: 广州医药 | 6-12 发布时间:2021-11-30 收稿时间:2025/11/13 17:16:21 阅读量:34
作者:
关键词:
高氧细胞凋亡WISP-3ALI/ARDS
HyperoxiaApoptosisWISP-3ALI/ARDS
DOI:
10.3969/j.issn.1000-8535.2018.06.002
收稿时间:
2018-08-04 
修订日期:
 
接收日期:
 
引用总数:
0  
目的 在原来研究的基础上进一步研究Wnt-1信号通路蛋白-3(WISP-3)在高氧诱导肺上皮细胞凋亡中的作用。方法 通过Western blot检测和免疫组化检测不同肺上皮细胞中WISP-3的蛋白表达量。利用质粒转染和siRNA的方法在Beas-2B细胞中高表达和基因沉默WISP-3,通过细胞活性检测和流式细胞学技术检测高氧刺激后细胞的凋亡情况。结果 与空气对照相比,高氧刺激使肺上皮细胞的WISP-3蛋白表达量下降;WISP-3基因沉默或高表达使高氧诱导的肺上皮细胞凋亡增加或减少。结论 高氧刺激下,肺上皮细胞中WISP-3表达下降,WISP-3对高氧诱导的肺上皮细胞凋亡具有保护作用。
Objective To explore how Wnt-1 inducible signaling pathway protein-3 (WISP-3) participate in and play a regulatory role in the process of hyperoxia induced apoptosis in lung epithelial cells. Methods The expression of WISP-3 was detected via Western blot and immunohistochemistry. High expression and low expression of WISP-3 were performed by plasmid transfection and siRNA. Cell viability and flow cytometry were executed to detect the hyperoxia-induced apoptosis in Beas-2B. Results Compared to the group of air control,the expression of WISP-3 protein in lung epithelial cells decreased obviously after hyperoxia. Cell survival decrease and apoptosis increased after hyperoxia in Beas-2B cells with low expression of WISP-3. Vice versa. Conclusion The expression of WISP-3 decreased after hyperoxia in lung epithelial cells. The role of WISP-3 in this process may be protective.
1、 KLEER C G,ZHANG Y,PAN Q,et al.WISP3 (CCN6) is a secreted tumor-suppressor protein that modulates IGF signaling in inflammatory breast cancer[J]. Neoplasia,2004 ,6(2):179-185. KLEER C G,ZHANG Y,PAN Q,et al.WISP3 (CCN6) is a secreted tumor-suppressor protein that modulates IGF signaling in inflammatory breast cancer[J]. Neoplasia,2004 ,6(2):179-185.
2、 GOTTLIEB T M,LEAL J F,SEGER R,et al.Cross-talk between Akt,p53 and Mdm2: possible implications for the regulation of apoptosis[J]. Oncogene,2002,21(8):1299. GOTTLIEB T M,LEAL J F,SEGER R,et al.Cross-talk between Akt,p53 and Mdm2: possible implications for the regulation of apoptosis[J]. Oncogene,2002,21(8):1299.
3、 GOLEN K L V,DAVIES S,WU Z F,et al. A novel putative low-affinity insulin-like growth factor-binding protein,lIBC (lost in inflammatory breast cancer),and rhoc gtpase correlate with the inflammatory breast cancer phenotype[J]. clin cancer res,1999,5(9):2511-2519. GOLEN K L V,DAVIES S,WU Z F,et al. A novel putative low-affinity insulin-like growth factor-binding protein,lIBC (lost in inflammatory breast cancer),and rhoc gtpase correlate with the inflammatory breast cancer phenotype[J]. clin cancer res,1999,5(9):2511-2519.
4、 KLEER C G,ZHANG Y,PAN Q,et al.WISP3 (CCN6) Is a secreted tumor-suppressor protein that modulates igf signaling in inflammatory breast cancer[J]. Neoplasia,2004,6(2):179. KLEER C G,ZHANG Y,PAN Q,et al.WISP3 (CCN6) Is a secreted tumor-suppressor protein that modulates igf signaling in inflammatory breast cancer[J]. Neoplasia,2004,6(2):179.
5、 HUANG W,GONZALEZ M E,TOY K A,et al.Blockade of CCN6 (WISP3) activates growth factor-independent survival and resistance to anoikis in human mammary epithelial cells[J]. Cancer Res,2010,70(8):3340-3350. HUANG W,GONZALEZ M E,TOY K A,et al.Blockade of CCN6 (WISP3) activates growth factor-independent survival and resistance to anoikis in human mammary epithelial cells[J]. Cancer Res,2010,70(8):3340-3350.
6、 MILLER D S,SEN M.Potential role of WISP3 (CCN6) in regulating the accumulation of reactive oxygen species[J]. Biochem Biophys Res Commun,2007,355(1):156-161. MILLER D S,SEN M.Potential role of WISP3 (CCN6) in regulating the accumulation of reactive oxygen species[J]. Biochem Biophys Res Commun,2007,355(1):156-161.
7、 Milan P, Sushil K M,Deepsh K,et al.CCN6 regulates mitochondrial function[J]. J Cell Sci ,2016,129(14):2841. Milan P, Sushil K M,Deepsh K,et al.CCN6 regulates mitochondrial function[J]. J Cell Sci ,2016,129(14):2841.
8、 解立新. 高氧对呼吸危重症患者的危害及氧疗规范[J]. 中华医学杂志,2017,97(20): 1529-1530. 解立新. 高氧对呼吸危重症患者的危害及氧疗规范[J]. 中华医学杂志,2017,97(20): 1529-1530.
9、 DELL'ANNA A M,LAMANNA I,VINCENT J L,et al. How much oxygen in adult cardiac arrest?[J].Crit Care,2014,18(5):555. DELL'ANNA A M,LAMANNA I,VINCENT J L,et al. How much oxygen in adult cardiac arrest?[J].Crit Care,2014,18(5):555.
10、 曹洁,董丽霞,陈宝元. 规避高氧危害规范目标氧疗[J]. 中华结核呵呵呼吸杂志,2015,38(8):629-631. 曹洁,董丽霞,陈宝元. 规避高氧危害规范目标氧疗[J]. 中华结核呵呵呼吸杂志,2015,38(8):629-631.
11、 张静,陈宝元. 临床氧疗相关指南简介及解读[J]. 中华医学杂志,2017,97(20):1540-1544. 张静,陈宝元. 临床氧疗相关指南简介及解读[J]. 中华医学杂志,2017,97(20):1540-1544.
12、 WEI S,MOON H G,ZHENG Y,et al.Flotillin-2 modulates fas signaling mediated apoptosis after hyperoxia in lung epithelial cells[J]. PLoS One,2013,8(10):e77519. WEI S,MOON H G,ZHENG Y,et al.Flotillin-2 modulates fas signaling mediated apoptosis after hyperoxia in lung epithelial cells[J]. PLoS One,2013,8(10):e77519.
13、 曹洁,刘丹. 高氧对机体的损害及发生机制[J]. 中华医学杂志,2017,97(20):1537-1539. 曹洁,刘丹. 高氧对机体的损害及发生机制[J]. 中华医学杂志,2017,97(20):1537-1539.
14、 GIRARDIS M,BUSANI S,DAMIANI E,et al.Effect of conservative vs conventional oxygen therapy on mortality among patients in an intensive care unit: the oxygen-ICU randomized clinical trial[J]. JAMA,2016 ,316(15):1583-1589. GIRARDIS M,BUSANI S,DAMIANI E,et al.Effect of conservative vs conventional oxygen therapy on mortality among patients in an intensive care unit: the oxygen-ICU randomized clinical trial[J]. JAMA,2016 ,316(15):1583-1589.
15、 CHU D K,KIM L H,YOUNG P J,et al.Mortality and morbidity in acutely ill adults treated with liberal versus conservative oxygen therapy (IOTA): a systematic review and meta-analysis[J]. Lancet, 2018,391(10131):1693-1705. CHU D K,KIM L H,YOUNG P J,et al.Mortality and morbidity in acutely ill adults treated with liberal versus conservative oxygen therapy (IOTA): a systematic review and meta-analysis[J]. Lancet, 2018,391(10131):1693-1705.
16、 ESTEBAN A,ANZUETO A,FRUTOS F,et al.Characteristics and outcomes in adult patients receiving mechanical ventilation: a 28-day international study[J]. JAMA,2002,287(3):345-355. ESTEBAN A,ANZUETO A,FRUTOS F,et al.Characteristics and outcomes in adult patients receiving mechanical ventilation: a 28-day international study[J]. JAMA,2002,287(3):345-355.
上一篇
下一篇
出版者信息








《广州医药》公众号
目录