雷公藤红素对阿霉素耐药乳腺癌细胞生长的抑制作用研究

来源期刊：广州医药 | 42-45 发布时间：2021-12-01 收稿时间：2025/11/13 17:10:52 阅读量：21

作者：: 谢闺娥,

杜晶春,

徐霞,

关键词：: 阿霉素耐药雷公藤红素 MCF-7/ADR 凋亡细胞周期; Adriamycin resistance Celastrol MCF-7/ADR Apoptosis Cell cycle

DOI：: 10.3969/j.issn.1000-8535.2017.04.010

收稿时间：: 2017-03-25
修订日期：
接收日期：
引用总数：: 0

目的研究雷公藤红素对人阿霉素耐药MCF-7/ADR乳腺癌细胞生长的作用。方法采用MTT试验检测MCF-7/ADR细胞对阿霉素的耐药情况以及雷公藤红素对MCF-7/ADR细胞生长的影响;采用Annexin V-FITC/PI双染试验分析雷公藤红素对MCF-7/ADR耐药细胞凋亡的诱导作用;应用流式细胞周期分析检测雷公藤红素对MCF-7/ADR耐药细胞周期的影响。结果 MCF-7/ADR细胞对阿霉素耐药指数达14.54;而雷公藤红素能有效抑制阿霉素耐药细胞MCF-7/ADR的生长,并呈现浓度依赖性,作用48 h的IC50是1.04 μmol/L,MCF-7/ADR对雷公藤红素的耐药指数仅为0.87。2 μmol/L雷公藤红素作用8 h后,Annexin V-FITC染色阳性的MCF-7/ADR细胞比例较对照显著升高,差异有统计学意义(P＜0.05)。在1 μmol/L雷公藤红素作用24 h后,G1期细胞比例由对照(59.22±3.78)%升高至(77.44±4.21)%,而S期细胞比例由对照(37.51±2.91)%降至(19.65±2.25)%,差异有统计学意义(P＜0.05)。结论雷公藤红素能激活MCF-7/ADR细胞凋亡的发生,并诱导MCF-7/ADR发生 G1/S细胞周期阻滞,从而对阿霉素耐药MCF-7/ADR细胞的生长发挥高效抑制作用。

1、 CANAVESE M, SANTO L, RAJE N. Cyclin dependent kinases in cancer: potential for therapeutic intervention[J]. Cancer Biol Ther, 2012,13(7):451-457.

2、 SUI X, KONG N, YE L, et al. p38 and JNK MAPK pathways control the balance of apoptosis and autophagy in response to chemotherapeutic agents[J]. Cancer Lett, 2014,344(2):174-179.

3、 GHOBRIAL I M, WITZIG T E, ADJEI A A. Targeting apoptosis pathways in cancer therapy[J]. CA Cancer J Clin, 2005,55(3):178-194.

4、 LI P P, HE W, YUAN P F, et al. Celastrol induces mitochondria-mediated apoptosis in hepatocellular carcinoma Bel-7402 cells[J]. Am J Chin Med, 2015,43(1):137-148.

5、 MA J, HAN L Z, LIANG H, et al. Celastrol inhibits the HIF-1α pathway by inhibition of mTOR/p70S6K/eIF4E and ERK1/2 phosphorylation in human hepatoma cells[J]. Oncol Rep, 2014, 32(1): 235-242.

6、 ZHAO X, GAO S, REN H, et al. Inhibition of autophagy strengthens celastrol-induced apoptosis in human pancreatic cancer in vitro and in vivo models[J]. Curr Mol Med, 2014,14(4): 555-563.

7、 LEE H W, JANG K S, CHOI H J, et al. Celastrol inhibits gastric cancer growth by induction of apoptosis and autophagy[J]. BMB Rep, 2014,47(12):697-702.

8、全军利,贺文兴,吴斯敏,等.STAT3基因沉默对乳腺癌阿霉素耐药性的影响[J]. 实用医学杂志, 2015, 31(11):1748-1751.

9、 YU P, YU H, GUO C, et al. Reversal of doxorubicin resistance in breast cancer by mitochondria-targeted pH-responsive micelles[J]. Acta Biomater, 2015,14:115-124.

10、 FRIEDENREICH C M. Physical activity and breast cancer: review of the epidemiologic evidence and biologic mechanisms[J]. Recent Results Cancer Res, 2011,188:125-139.