EGF在新生儿坏死性小肠结肠炎中肠道定位和表达特征

来源期刊：广州医药 | 14-18 发布时间：2021-12-01 收稿时间：2025/11/13 17:09:05 阅读量：24

作者：: 农一敏,

何秋明,

宋燕燕,

夏慧敏,

吕俊健,

关键词：: 表皮生长因子新生儿坏死性小肠结肠炎免疫组织化学; Epidermal growth factor Neonatal necrotizing enterocolitis Immunohistochemistry

DOI：: 10.3969/j.issn.1000-8535.2017.05.004

收稿时间：: 2017-04-07
修订日期：
接收日期：
引用总数：: 0

目的观察表皮生长因子(EGF)在新生儿坏死性小肠结肠炎(NEC)患儿肠组织中的动态表达情况,探讨EGF在NEC病程中起到的保护作用。方法选取15例NEC患儿行一期回肠造瘘手术治疗的回肠组织为实验组(NEC组),将以上15例NEC患儿行二期回肠封瘘手术治疗的回肠组织为对照组(封瘘组),采用免疫组织化学技术检测,通过光密度测算软件(IPP)分析回肠组织中的EGF表达。结果 EGF主要表达于肠壁黏膜层,少量表达于黏膜下层、肌层。EGF在NEC组各层表达平均光密度值为:黏膜层(0.241±0.075),黏膜下层(0.213±0.061),肌层(0.1397±0.026),差异有统计学意义(P＜0.05);在封瘘组各层表达情况为:黏膜层(0.211±0.028),黏膜下层(0.119±0.022),肌层(0.097±0.007),差异有统计学意义(P＜0.05)。EGF在NEC组总体表达平均光密度值为(0.198±0.071),明显高于封瘘组(0.146±0.058),差异有统计学意义(P＜0.05)。结论表皮生长因子(EGF)在新生儿坏死性小肠结肠炎(NEC)肠组织中的表达较封瘘组显著上调,推测EGF可能与NEC炎症相关,可能在NEC炎症过程中起到了一定的保护作用。

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