Objective To explore the relationship between helicobacter pylori infection and reflux esophagitis. Methods 364 patients with reflux esophagitis were enrolled in our hospital from Jan to Dec in 2010.The severity grade of reflux esophagitis was according to Los Angles standard. All the patients received biopsies from gastric antrum,then received pathologic examinations. Results Reflux esophagitis in men was more common, with an average age of onset earlier than the female(52.4±11.6 years vs 56.6±12.4 years,P=0.002);Reflux esophagitis in patients with H.pylori infection rate was 90.9% overall,mainly with mild infection,four groups of H.pylori infection rate and extent of infection was no significant difference (P values were 0.419,0.702);reflux esophagitis in patients with chronic superficial gastritis(CSG),moderate active inflammation based was more common,there was no statistically significant difference in four group(P values were 0.146,0.496). There was no significant difference between H.pylori positive patients and H.pylori negative patients in esophageal mucosal damage(P> 0.05). There was no significant difference in reflux esophagitis with varying degrees of H.pylori infection on the degree of esophagitis. Conclusion Four groups of H.pylori infection rate and extent of infection was no significant difference; there was also no significant difference between H.pylori positive patients and H.pylori negative patients in esophageal mucosal damage. There was no significant difference in RE with varying degrees of H.pylori infection on the degree of esophagitis. Our research showed H.pylori infection had no relationship with degree of RE.