Objective To investigate the applicative value of Propofol intravenous anesthesia combining with local infiltration anesthesia in vacuum assisted biopsy minimal invasive system for multiple breast lumps excision. Methods Encor minimally invasive surgery was performed under Propofol intravenous anesthesia together with local infiltration anesthesia in 380 cases with multiple breast lumps admitted to our department from March 2012 to March 2015. Anesthesia effect, SpO₂, MAP and HR were analyzed retrospectively. Results All 380 patients achieved good anesthetic effect for completing multiple breast lumps excision. The postoperative complications were less. There was no obvious change of oxyhemoglobin saturation (SpO₂) before, during and after the surgery period (P>0.05). The mean arterial pressure (MAP) and heart rate (HR) had statistical significance compared the surgery period with the pre-operative time (P<0.05). Conclusion Vacuum assisted biopsy minimal invasive system under Propofol intravenous anesthesia combining with local infiltration anesthesia is a safety and feasible method for multiple breast lumps excision. It has more advantages including perfect anesthetic effect, good patient compliance, higher satisfaction and higher tolerance level.