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miR-221在前列腺癌细胞中的表达及对增殖的影响

Effect of miR-221 expression on proliferation in prostate cancer cells

来源期刊: 广州医药 | 1-3 发布时间:2021-12-02 收稿时间:2025/11/13 16:54:14 阅读量:14
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关键词:
前列腺癌miR-221细胞增殖
Prostate cancermiR-221Cell proliferation
DOI:
10.3969/j.issn.1000-8535.2016.05.001
收稿时间:
2016-04-05 
修订日期:
 
接收日期:
 
引用总数:
0  
目的 研究前列腺癌细胞中miR-221的表达情况及其对癌细胞增殖的影响。方法 运用实时荧光定量PCR(qRT-PCR)检测miR-221在前列腺正常细胞株与前列腺癌细胞株中表达的差异情况,利用细胞转染构建miR-221过表达LNCaP和DU145细胞株,再通过CCK8细胞增殖实验检测细胞增殖情况的变化。结果 qRT-PCR检测细胞株发现miR-221在PC3、LNCaP和DU145三种前列腺癌细胞株中表达量均比前列腺正常细胞株PrEC低 (F=254.197,P<0.001),其中两两比较差异也均有统计学意义。细胞转染技术构建的miR-221过表达LNCaP和DU145细胞株,经qRT-PCR结果显示,miR-221在LNCaP和DU145细胞株中的表达水平明显升高(LNCaP,倍数变化=2.24,t=3.46,P<0.01;Du145,倍数变化=2.24,t=4.29,P<0.01)。细胞增殖实验结果显示,过表达了miR-221的LNCaP(P<0.001)和DU145(P<0.001)细胞生长速度慢于对照组。结论 实验证明miR-221表达过度能减慢前列腺癌细胞的增殖,miR-221有可能成为前列腺肿瘤治疗的生物学标志物。
Objective To investigate miR-221 expression in prostate cancer cells and its influence on prostate cancer cell proliferation. Methods miR-221 expressions in prostate normal cell lines and cancer cell lines were measured by qRT-PCR. Overexpression of the miR-221 in LNCaP and DU145 cell lines used by cell transfection. Effects of the depletion on cell proliferation were assessed in vitro with CCK8. Results qRT-PCR showed miR-221 was lower expressed in PC3, LNCaP and DU145 than in PrEC(F=254.197, P<0.001), in which pairwise comparison also had significant differences. qRT-PCR showed miR-221 expression rose significantly in LNCaP and DU145 cell lines whose miR-221 was overexpression with cell transfection(LNCaP, Fold Change=2.24,t=3.46,P<0.001;Du145, Fold Change=2.24,t=4.29,P<0.001). Cell proliferation assay showed that growth of LNCaP(P<0.001) and DU145(P<0.001) cells whose miR-221 was overexpression was slower than the control group. Conclusion This study demonstrates miR-221 overexpression can inhibited the proliferation of prostate cancer cells for the first time, it also suggests that miR-221 has the potential to serve as a biomarker for PCa therapy.
1、 CARROLL AP, GOODALL GJ, LIU B. Understanding principles of miRNA target recognition and function through integrated biological and bioinformatics approaches[J]. Wiley Interdiscip Rev RNA,2014,5(3):361-379. CARROLL AP, GOODALL GJ, LIU B. Understanding principles of miRNA target recognition and function through integrated biological and bioinformatics approaches[J]. Wiley Interdiscip Rev RNA,2014,5(3):361-379.
2、 GRIFFITHS-JONES S. The miRNA registry[J]. Nucl Acids Res, 2004,32: D109-111. GRIFFITHS-JONES S. The miRNA registry[J]. Nucl Acids Res, 2004,32: D109-111.
3、 DUARTE FV, PALMEIRA CM, ROLO AP. The Emerging Role of MitomiRs in the Pathophysiology of Human Disease[J]. Adv Exp Med Biol, 2015,888:123-154. DUARTE FV, PALMEIRA CM, ROLO AP. The Emerging Role of MitomiRs in the Pathophysiology of Human Disease[J]. Adv Exp Med Biol, 2015,888:123-154.
4、 GORDANPOUR A, STANIMIROVIC A, NAM RK, et al. miR-221 Is down-regulated in TMPRSS2:ERG fusion-positive prostate cancer[J]. Anticancer Res, 2011,31(2):403-410. GORDANPOUR A, STANIMIROVIC A, NAM RK, et al. miR-221 Is down-regulated in TMPRSS2:ERG fusion-positive prostate cancer[J]. Anticancer Res, 2011,31(2):403-410.
5、 TOMLINS SA, RHODES DR, PERNER S, et al. Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer. Science, 2015, 310 (5748):644-648. TOMLINS SA, RHODES DR, PERNER S, et al. Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer. Science, 2015, 310 (5748):644-648.
6、 GU Y, LEI D, QIN X,et al. Integrated Analysis Reveals together miR-182, miR-200c and miR-221 Can Help in the Diagnosis of Prostate Cancer[J]. PLoS On,2015,10(10):e0140862. GU Y, LEI D, QIN X,et al. Integrated Analysis Reveals together miR-182, miR-200c and miR-221 Can Help in the Diagnosis of Prostate Cancer[J]. PLoS On,2015,10(10):e0140862.
7、 LI B, LU Y, WANG H,et al. miR-221/222 enhance the tumorigenicity of human breast cancer stem cells via modulation of PTEN/Akt pathway[J]. Biomed Pharmacother,2016,79:93-101. LI B, LU Y, WANG H,et al. miR-221/222 enhance the tumorigenicity of human breast cancer stem cells via modulation of PTEN/Akt pathway[J]. Biomed Pharmacother,2016,79:93-101.
8、 CAI C, CHEN QB, HAN ZD, et al. miR-195 inhibits tumor progression by targeting RPS6KB1 in human prostate cancer[J]. Clin Cancer Res, 2015, 21(21):4922-4934. CAI C, CHEN QB, HAN ZD, et al. miR-195 inhibits tumor progression by targeting RPS6KB1 in human prostate cancer[J]. Clin Cancer Res, 2015, 21(21):4922-4934.
9、 DEMARZO AM, NELSON WG, ISAACS WB, et al. Pathological and molecular aspects of prostate cancer[J]. Lancet,2013,361:955-964. DEMARZO AM, NELSON WG, ISAACS WB, et al. Pathological and molecular aspects of prostate cancer[J]. Lancet,2013,361:955-964.
10、 SIEGEL RL, MILLER KD, JEMAL A. Cancer statistics, 2016[J]. CA Cancer J Clin,2016,66(1):7-30. SIEGEL RL, MILLER KD, JEMAL A. Cancer statistics, 2016[J]. CA Cancer J Clin,2016,66(1):7-30.
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