高浓度二甲双胍通过JNK通路抑制MIN6细胞增殖和迁移

doi:10.3969/j.issn.1000-8535.2018.01.001

广州医药 ›› 2018, Vol. 49 ›› Issue (1): 1-4.DOI: 10.3969/j.issn.1000-8535.2018.01.001

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高浓度二甲双胍通过JNK通路抑制MIN6细胞增殖和迁移

汪奇峰, 韦晓, 陈煜, 陈国芳, 茅晓东, 刘超

南京中医药大学附属中西医结合医院,江苏省中医药研究院(南京 210028)

收稿日期:2017-08-30 出版日期:2018-01-20 发布日期:2021-12-01
通讯作者: 刘超,E-mail:liuchao@nfmcn.com
基金资助:
江苏省科技计划项目(BK20141037)

High-concentration metformin inhibits the proliferation and migration of MIN6 cells through JNK signaling pathway

WANG Qifeng, WEI Xiao, CHEN Yu, CHEN Guofang, MAO Xiaodong, LIU Chao

Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine; Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China

Received:2017-08-30 Online:2018-01-20 Published:2021-12-01

摘要/Abstract

摘要： 目的本研究从细胞生物学角度检测二甲双胍对小鼠胰岛瘤MIN6的影响,并探讨此过程中包含的分子生物学机制。方法 MTT法检测不同浓度二甲双胍(1、2、5、10、20 mmol/L)对MIN6细胞活力的影响,细胞划痕实验检测二甲双胍对MIN6细胞迁移的影响,免疫印记实验检测此过程中细胞凋亡相关蛋白Bcl-2、Bax、caspase3表达的变化,及AMPK和JNK信号通路蛋白磷酸化水平的变化。结果二甲双胍浓度大于10 mmol/L时可以抑制MIN6细胞的活力(P<0.01),降低其迁移能力(P<0.01),高浓度二甲双胍可以上调细胞内凋亡蛋白Bax(P<0.05)和p-AMPK的表达(P<0.05),降低抗凋亡蛋白Bcl-2的表达,增加caspase3剪切体(P<0.05)。同时,二甲双胍可以降低MIN6细胞内JNK信号通路的磷酸化水平(P<0.05)。结论高浓度二甲双胍可以抑制MIN6细胞的增殖和迁移,其作用可能与降低了JNK信号的通路活化有关。

关键词: 二甲双胍, MIN6, 细胞凋亡, JNK

Abstract: Objective This study aims to investigate the effect of metformin on proliferation and migration of MIN6 cells, and to explore the underlying mechanism. Methods The viability of MIN6 cells that were treated with various metformin (1,2,5,10 and 20 mmol/L) was detected by MTT assay. The migration of MIN6 cells was determined by wound-healing assay. Meanwhile, the proteins expression of Bcl-2, Bax, caspase3 and the phosphorylation of AMPK, JNK was detected by western bolt assay. Results The cell viability and the migration of MIN6 cells were decreased when the concentration of metformin above 10 mmol/L(P<0.01). The expression of apoptosis-related protein Bax(P<0.05) and p-AMPK(P<0.05)was up-regulated, anti-apoptosis-related protein Bcl-2 was down-regulated and cleaved caspase3 (P<0.05)was increased after high metformin treatment. At the same time, the phosphorylation of JNK was down-regulated by metformin(P<0.05). Conclusion High concertration of metformin may inhibit the proliferation and migration of MIN6 cells through suppressing the activation of JNK signaling pathway.

Key words: Metformin, MIN6, Apoptosis, JNK

汪奇峰, 韦晓, 陈煜, 陈国芳, 茅晓东, 刘超. 高浓度二甲双胍通过JNK通路抑制MIN6细胞增殖和迁移[J]. 广州医药, 2018, 49(1): 1-4.

WANG Qifeng, WEI Xiao, CHEN Yu, CHEN Guofang, MAO Xiaodong, LIU Chao. High-concentration metformin inhibits the proliferation and migration of MIN6 cells through JNK signaling pathway[J]. Guangzhou Medical Journal, 2018, 49(1): 1-4.

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高浓度二甲双胍通过JNK通路抑制MIN6细胞增殖和迁移

High-concentration metformin inhibits the proliferation and migration of MIN6 cells through JNK signaling pathway

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