高脂血症大鼠血液中氨基酸代谢标志物分析

doi:10.3969/j.issn.1000-8535.2020.06.001

广州医药 ›› 2020, Vol. 51 ›› Issue (6): 1-5.DOI: 10.3969/j.issn.1000-8535.2020.06.001

• 论著 • 下一篇

高脂血症大鼠血液中氨基酸代谢标志物分析

江洁怡¹, 肖观林¹, 曾志浩², 蔡大可¹

1 广东省中医药工程技术研究院/广东省中医药研究开发重点实验室(广州 510095)
2 广州中医药大学第五临床医学院(广州 510405)

收稿日期:2020-04-29 出版日期:2020-11-20 发布日期:2021-11-28
基金资助:
广东省科技计划项目(2017A070701017);国家重大科技专项(2016ZX09101076); 广东省医学科学技术研究基金项目(B2018285)

Preliminary study on amino acid metabolism markers in blood of hyperlipidemia rats

JIANG Jieyi¹, XIAO Guanlin¹, ZENG Zhihao², CAI Dake¹

1 Guangdong Province Engineering Technology Research Institute of Traditional Chinese Medicine/Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine, Guangzhou 510095, China
2 The Fifth Clinical Medicine College Guangzhou University of Chinese Medicine, Guangzhou 510405, China

Received:2020-04-29 Online:2020-11-20 Published:2021-11-28

摘要/Abstract

摘要： 目的探讨高脂血症大鼠模型前后血液中氨基酸代谢谱的变化,寻找高脂血症大鼠血液中氨基酸代谢标志物。方法将SD大鼠随机分为正常对照组、模型组,连续灌胃给药4周后收集大鼠血液,测定各组大鼠血清中TG、TC、HDL-C、LDL-C含量,并运用超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF-MS/MS)法测定血清中氨基酸代谢谱,利用统计学分析研究不同组动物间的氨基酸代谢的差异。结果与正常对照组比较,模型组TG、TC、LDL-C含量升高,HDL-C含量降低,高脂血症大鼠模型建模成功;与正常对照组比较,模型组蛋氨酸、苯丙氨酸、脯氨酸、苏氨酸、缬氨酸、甘氨酸等6种氨基酸发生明显改变(P<0.05)。结论高脂血症大鼠存在氨基酸代谢的紊乱,其中蛋氨酸、苯丙氨酸、脯氨酸、苏氨酸、缬氨酸、甘氨酸等6种氨基酸为其潜在的生物标志物。

关键词: 高脂血症, UPLC-Q-TOF-MS/MS, 氨基酸代谢, 标志物

Abstract: Objective To investigate the amino acid metabolism profiles changes in the serum of SD rats, and identify the potential biomarkers. Methods SD rats were divided into normal group and model group. The contents of TG, TC, HDL-C, and LDL-C in the serum of each group were measured, after 4 weeks of continuous intragastric administration. Ultra performance liquid chromatography coupled with electrospray time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS)was used to determine amino acid metabolism profile in serum, and statistical analysis was applied to determine metabolic differences among different groups of rats. Results As compared with normal group, TG, TC, LDL-C were increased and HDL-C was decreased in model group, hyperlipidemia rat model successfully modeled. As compared with normal group, methionine, phenylalanine, proline, threonine, valine, glycine in the amino acid metabolic profiling were decreased in model group (P＜0.05). Conclusion Hyperlipidemia rats have disorders of amino acid metabolism, of which methionine, phenylalanine, proline, threonine, valine, and glycine are potential biomarkers.

Key words: Hyperlipidemia, UPLC-Q-TOF-MS/MS, Amino acid metabolism, Biomarkers

江洁怡, 肖观林, 曾志浩, 蔡大可. 高脂血症大鼠血液中氨基酸代谢标志物分析[J]. 广州医药, 2020, 51(6): 1-5.

JIANG Jieyi, XIAO Guanlin, ZENG Zhihao, CAI Dake. Preliminary study on amino acid metabolism markers in blood of hyperlipidemia rats[J]. Guangzhou Medical Journal, 2020, 51(6): 1-5.

参考文献

[1] 刘冲. 高脂血症的证型特点研究[D]. 福州: 福建中医药大学, 2016.
[2] 王远红, 徐家敏, 耿美玉, 等. 海洋硫酸多糖916对实验性高脂血症大鼠血浆中含硫氨基酸的影响[J]. 中国海洋药物, 2000(4): 23-24.
[3] 苗华. 基于代谢组学技术研究泽泻对高脂血症大鼠防治的生物化学作用机制[J], 西北大学学报(自然科学版), 2016, 46(3): 397-402.
[4] 罗朵生, 李坤平, 朴胜华, 等. 高脂血症大鼠造模过程中血液代谢组学研究[J]. 中药新药与临床药理, 2016, 27(1): 70-74.
[5] 冯雷, 陈章玉, 王保兴, 等. 柱前衍生化反相高效液相色谱法测定烟草中的游离氨基酸[J]. 云南大学学报: 自然科学版, 2003, 25(S1): 241-245.
[6] 宋彦强, 夏黎黎, 李玮, 等. 血液中氨基酸检测方法研究进展[J]. 检验医学与临床, 2013, 10(24): 3361-3362.
[7] OGLESBEE D, SANDERS K A, LACEY J M, et al, Second-tier test for quantification of alloisoleucine and branched-chain amino acids in dried blood spots to improve newborn screening for maple syrup urine disease (MSUD)[J]. Clin Chem, 2008, 54(3): 542-549.
[8] ALADDIN M, SAPNA G, HUDES G R, et al.Serum amino acid levels as a biomarker for renal cell carcinoma[J]. J Urol, 2011, 86(4):1206-1212.
[9] LEICHTLE A B, NUOFFER J M, CEGLAREK U, et al. Serum amino acid profiles and their alterations in colorectal cancer[J]. Metabolomics, 2012, 8(4):643-653.
[10] 段满乐, 伦立民. 缺血性脑血管疾病患者血浆氨基酸递质的测定及其意义[J]. 中华检验医学杂志, 2005, 28(12): 1256-1257.
[11] YUAN Y S, ZHOU X J, TONG Q, et al. Change in plasma levels of amino acid neurotransmitters and its correlation with clinical heterogeneity in early Parkinson＇s disease patients[J]. CNS Neurosci Ther, 2013, 19(11): 889-896.
[12] NAGATA C, WADA K, TSUJI M, et al. Plasma amino acid profiles are associated with biomarkers of breast cancer risk in premenopausal Japanese women[J]. Cancer Causes Control, 2014, 25(2): 143-149.
[13] MAGNUSSON M, LEWIS G D, ERICSON U, et al. A diabetes -predictive amino acid score and future cardiovascular disease[J]. Eur Heart J, 2013, 34(26): 1982-1989.

高脂血症大鼠血液中氨基酸代谢标志物分析

Preliminary study on amino acid metabolism markers in blood of hyperlipidemia rats

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价