iTRAQ联合质谱技术筛选COPD大鼠肺组织差异表达蛋白

doi:10.3969/j.issn.1000-8535.2020.06.002

广州医药 ›› 2020, Vol. 51 ›› Issue (6): 6-10.DOI: 10.3969/j.issn.1000-8535.2020.06.002

iTRAQ联合质谱技术筛选COPD大鼠肺组织差异表达蛋白

卢心鹏^1,2, 廖东江^1,2, 罗钰龙^1,2, 潘俊辉¹, 陈鸿生^1,2, 王峰¹

1 广州医科大学附属第一医院(广州 510120)
2 广州呼吸健康研究院呼吸疾病国家重点实验室(广州 510120)

收稿日期:2020-04-27 出版日期:2020-11-20 发布日期:2021-11-28
通讯作者: 王峰,E-mail:412475734@qq.com
基金资助:
广州市医药卫生科技项目(20161A010076)

Screening of differentially expressed proteins in the lung of COPD rats by iTRAQ and mass spectrometry

LU Xinpeng^1,2, LIAO Dongjiang^1,2, LUO Yulong^1,2, PAN Junhui¹, CHEN Hongsheng^1,2, WANG Feng¹

1 First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China
2 State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Diseases, Guangzhou 510120, China

Received:2020-04-27 Online:2020-11-20 Published:2021-11-28

摘要/Abstract

摘要： 目的应用iTRAQ联合质谱技术筛选COPD大鼠肺组织差异表达蛋白。方法 20只雄性SD大鼠(200~220 g),随机分为对照组和模型组,每组10只,采用熏烟法建立COPD大鼠模型。观察大鼠肺组织病理学改变,测定肺功能,BALF白细胞数,肺组织总蛋白iTRAQ标记后质谱鉴定,用生物信息学方法分析蛋白表达变化。结果与对照组相比,模型组大鼠支气管黏膜下肌层增厚,肺内可见大量炎性细胞浸润,肺功能降低,BALF白细胞数升高(均P<0.05)。质谱鉴定出4 916种蛋白,筛选出468个差异表达蛋白,其中285个表达上调,183个表达下调。筛选了上皮细胞粘着连接蛋白、fMLP、整合素等与COPD相关蛋白。结论基于iTRAQ技术的蛋白质组学方法筛选出COPD大鼠差异表达蛋白,为进一步研究COPD的发生机制奠定了基础。

关键词: 慢性阻塞性肺疾病, iTRAQ, 差异表达蛋白

Abstract: Objective iTRAQ and mass spectrometry were used to screen the differentially expressed proteins in the lung of COPD rats. Methods 20 male SD rats (200-220g)were randomly divided into control group and treatment group, with 10 rats in each group. COPD rat model was established by smoking. The lung function, the number of BALF leukocytes, the total protein iTRAQ in lung tissue were measured and identified by mass spectrometry. The differentially expressed proteins were identified by bioinformatic analysis. Results Compared with the control group, the submucous layer of bronchus in the model group was thickened, a large number of inflammatory cells were seen in the lung, the lung function was reduced, and the number of BALF leukocytes was increased. 4 916 proteins were identified by mass spectrometry, 468 differentially expressed proteins were screened, 285 of which were up-regulated and 183 down regulated. Among them, the important COPD related proteins were epithelial adhesion connexin, fMLP and integrins. Conclusion iTRAQ technology screened out the differentially expressed proteins of COPD rats, which laid the foundation for the further study of COPD mechanism

Key words: Chronic obstructive pulmonary disease, iTRAQ, Differential expressed protein

卢心鹏, 廖东江, 罗钰龙, 潘俊辉, 陈鸿生, 王峰. iTRAQ联合质谱技术筛选COPD大鼠肺组织差异表达蛋白[J]. 广州医药, 2020, 51(6): 6-10.

LU Xinpeng, LIAO Dongjiang, LUO Yulong, PAN Junhui, CHEN Hongsheng, WANG Feng. Screening of differentially expressed proteins in the lung of COPD rats by iTRAQ and mass spectrometry[J]. Guangzhou Medical Journal, 2020, 51(6): 6-10.

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iTRAQ联合质谱技术筛选COPD大鼠肺组织差异表达蛋白

Screening of differentially expressed proteins in the lung of COPD rats by iTRAQ and mass spectrometry

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