低氧预处理人胎盘绒毛膜间充质干细胞环状RNAs的生物信息学分析

doi:10.3969/j.issn.1000-8535.2019.02.001

广州医药 ›› 2019, Vol. 50 ›› Issue (2): 1-6.DOI: 10.3969/j.issn.1000-8535.2019.02.001

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低氧预处理人胎盘绒毛膜间充质干细胞环状RNAs的生物信息学分析

郝文革^1,2, 孙逊沙³, 吴洁莹^1,2, 陈劲松^1,2, 喻秋霞^1,2, 李焱^1,2, 吴韶清^1,2

1 广州医科大学附属广州市妇女儿童医疗中心(广州 510623)
2 中山大学附属广州市妇女儿童医疗中心(广州 510623)
3 中山大学附属第一医院(广州 510080)

收稿日期:2018-11-10 出版日期:2019-03-20 发布日期:2021-12-17
通讯作者: 吴韶清,E-mail:wushaoqing2005@21cn.com
基金资助:
国家自然科学基金项目(81601004,81102248);广东省自然科学基金(2016A030310139);广州市妇女儿童医疗中心儿科研究所基金(YIP-2016-014)

Bioinformatic analysis of circular RNAs in human placenta chorionic mesenchymal stem cells pretreated with hypoxia

HAO Wenge^1,2, SUN Xunsha³, WU Jieying^1,2, CHEN Jinsong^1,2, YU Qiuxia^1,2, LI Yan^1,2, WU Shaoqing^1,2

1 Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China
2 Guangzhou Women and Children's Medical Center, Sun Yat-sen University, Guangzhou 510623, China
3 First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China

Received:2018-11-10 Online:2019-03-20 Published:2021-12-17

摘要/Abstract

摘要： 目的采用生物信息学方法预测低氧预处理人胎盘绒毛膜间充质干细胞环状RNAs相对应的miRNA及其靶基因,并分析靶基因所参与的生物学过程和信号通路。方法用Arraystar公司的商业软件为环状RNAs预测其相对应的miRNAs,分别用targetScan7.1和mirdbV5数据库预测miRNAs的靶基因,并取两个预测结果的合集,应用在线网站http://www.geneontology.org和http://www.genome.ad.jp/kegg对靶基因进行功能富集分析和信号通路富集分析。结果功能富集分析表明,circRNAs的靶基因主要涉及到细胞发育、细胞分化和细胞发育调控。东京基因和基因组百科全书信号通路富集分析表明肿瘤中转录失控和有丝分裂原激活蛋白激酶(MAPK)信号通路最有意义,而且分析发现MAPK信号通路为核心通路。本研究表明,低氧预处理使得间充质干细胞中部分circRNAs的表达量发生差异性变化。结论低氧预处理人胎盘绒毛膜间充质干细胞环状RNAs同低氧预处理间充质干细胞的生物学特性变化密切有关,为了解低氧预处理影响间充质干细胞特性发生变化的分子机制提供新思路。

关键词: 环状circRNA, 低氧, 间充质干细胞, 生物信息学

Abstract: Objective To predict the miRNA and its target genes of circular RNAs in hypoxia- preconditioned human palcenta chorionic mesenchymal stem cells using bioinformatics, and analyze the biological process and signaling pathway. Methods Arraystar's commercial software was used to predict the corresponding miRNAs of circular RNAs. The target genes of miRNAs were predicted by targetScan7.1 and mirdbV5 databases respectively, and an intersection of two prediction results was obtained. The online databases http://www. geneontology.org and http://www.genome.ad.jp/kegg performed functional enrichment analysis and signal pathway enrichment analysis of target genes. Results Functional enrichment analysis indicated that the target genes of circRNAs mainly involved cell development, cell differentiation and cell development regulation. The signal enrichment analysis of the Tokyo Gene and Genome Encyclopedia indicates that transcriptional misregulation in cancer and mitogen-activated protein kinase (MAPK) signaling pathway are most meaningful, and the MAPK signaling pathway is found to be the core pathway. This study showed that hypoxic preconditioning caused significant changes in the expression of mesenchymal stem cell circRNAs. Conclusion The changes of circular RNAs in hypoxia-preconditioned human placental chorionic mesenchymal stem cell is closely related to the biological characteristics of hypoxia-preconditioned mesenchymal stem cells. This study provides a new idea for understanding the molecular mechanism of hypoxic preconditioning affecting the changes of biological characteristics in mesenchymal stem cells.

Key words: Circular RNAs, Hypoxia, Mesenchymal stem cells, Bioinformatics

郝文革, 孙逊沙, 吴洁莹, 陈劲松, 喻秋霞, 李焱, 吴韶清. 低氧预处理人胎盘绒毛膜间充质干细胞环状RNAs的生物信息学分析[J]. 广州医药, 2019, 50(2): 1-6.

HAO Wenge, SUN Xunsha, WU Jieying, CHEN Jinsong, YU Qiuxia, LI Yan, WU Shaoqing. Bioinformatic analysis of circular RNAs in human placenta chorionic mesenchymal stem cells pretreated with hypoxia[J]. Guangzhou Medical Journal, 2019, 50(2): 1-6.

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低氧预处理人胎盘绒毛膜间充质干细胞环状RNAs的生物信息学分析

Bioinformatic analysis of circular RNAs in human placenta chorionic mesenchymal stem cells pretreated with hypoxia

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