SHH、Gli-1、MMP-2在胃癌组织中的表达及临床意义

doi:10.3969/j.issn.1000-8535.2016.06.001

广州医药 ›› 2016, Vol. 47 ›› Issue (6): 1-5.DOI: 10.3969/j.issn.1000-8535.2016.06.001

• 论著 • 下一篇

SHH、Gli-1、MMP-2在胃癌组织中的表达及临床意义

陈世豪¹, 唐源¹, 卞永生²

1 承德医学院(承德 067000)
2 保定市第一医院(保定 071000)

收稿日期:2016-09-13 出版日期:2016-11-20 发布日期:2021-12-02

Expression and clinical significance of Shh,Gli-1 and MMP-2 in gastric cancer

Chen Shihao, Tang Yuan, Bian Yongsheng

Chen Shihao,Tang Yuan. Chengde Medical College, Chengde 067000,China
Bian Yongsheng. Baoding First Hospital, Baoding 071000,China

Received:2016-09-13 Online:2016-11-20 Published:2021-12-02

摘要/Abstract

摘要： 目的探讨胃癌组织中刺猬信号通路(Hedgehog signaling pathway, Hh)中的音猬因子(Sonic hedgehog, Shh)和胶质瘤相关癌基因同源物-1(Glioma-associated oncogene homolog -1, Gli-1)与金属基质蛋白酶-2(Matrix metalloproteinase-2, MMP-2)的表达和临床意义。方法采用免疫组织化学方法检测40例人胃癌组织、人胃息肉组织和40例正常胃黏膜组织中音猬因子、胶质瘤相关癌基因同源物-1、金属基质蛋白酶-2蛋白的表达。结果胃癌组织中音猬因子、胶质瘤相关癌基因同源物-1、金属基质蛋白酶-2的阳性表达率分别为62.5%、67.5%、72.5%,高于胃息肉组织(阳性表达率分别为27.5%、37.5%、32.5%)和正常胃黏膜组织(阳性表达率分别为22.5%、17.5%、12.5%)(P<0.05);以上三者的表达与患者性别、年龄、组织学类型无关(P>0.05);而与分化程度、浸润深度、淋巴结转移相关(P<0.05);音猬因子、胶质瘤相关癌基因同源物-1、金属基质蛋白酶-2表达呈正相关。结论刺猬信号通路可能通过某些机制可上调金属基质蛋白酶-2的表达,从而增强胃癌的侵袭性。联合检测胃癌组织中音猬因子、胶质瘤相关癌基因同源物-1、金属基质蛋白酶-2的表达水平在一定程度上可以作为胃癌预后的客观参考指标。

关键词: 胃癌, 音猬因子, 胶质瘤相关癌基因同源物-1, 金属基质蛋白酶-2, 免疫组织化学

Abstract: Objective To investigate the expression and clinical significance of Sonic hedgehog(Shh), Glioma-associated oncogene homolog -1(Gli-1) and Matrix metalloproteinase-2(MMP-2) in gastric cancer.Shh and Gli-1 are the molecules of Hedgehog(Hh) signaling pathway. MMP-2 is the member of matrix metalloproteinase family. Methods The expression of Shh,Gli-1 and MMP-2 proteins was examined by immunohistochemistry in the human gastric cancer tissues and the human gastric polyp and the normal gastric mucosa tissues of 40 cases. Results The positive expression rates of Shh,Gli-1 and MMP-2 in gastric cancer were 62.5%,67.5% and 72.5% respectively, which were significantly higher than those in the gastric polyp tissues (the positive expression rates were 27.5%,37.5% and 32.5% respectively) and normal gastric mucosa tissues (the positive expression rates were 22.5%,17.5% and 12.5% respectively),P<0.05.The expression of Shh,Gli-1 and MMP-2 was not correlated with the sex,age or histological type(P>0.05),but was correlated with depth of invasion,differentiation level and lymphonode metastasis in gastric cancer(P<0.05). The expression of Shh and Gli-1 was positive correlated with MMP-2. Conclusion Hedgehog(Hh)signaling pathway may have great effects on enhancing the invasive ability of gastric cancer by upregulating MMP-2 protein through some unknown mechanisms.The combined detection of the expression level of Shh,Gli-1 and MMP-2 in gastric cancer tissues might be used as an Objective references for assessing the prognosis of gastric cancer.

Key words: Gastric cancer, Sonic hedgehog, Glioma-associated oncogene homolog -1, Matrix metalloproteinase-2, Immunohistochemistry

陈世豪, 唐源, 卞永生. SHH、Gli-1、MMP-2在胃癌组织中的表达及临床意义[J]. 广州医药, 2016, 47(6): 1-5.

Chen Shihao, Tang Yuan, Bian Yongsheng. Expression and clinical significance of Shh,Gli-1 and MMP-2 in gastric cancer[J]. Guangzhou Medical Journal, 2016, 47(6): 1-5.

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SHH、Gli-1、MMP-2在胃癌组织中的表达及临床意义

Expression and clinical significance of Shh,Gli-1 and MMP-2 in gastric cancer

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