血糖波动对糖尿病胃轻瘫大鼠Cajal间质细胞的影响及其机制研究

doi:10.3969/j.issn.1000-8535.2016.02.001

摘要/Abstract

摘要： 目的探讨糖尿病胃轻瘫大鼠不同血糖水平对Cajal间质细胞(ICC)的影响及其机制。方法选择雌性Wista大鼠60只进行随机分组,实验组40只,对照组20只。实验组糖尿病Wista大鼠模型以单次腹腔注射链脲佐菌素法诱导。免疫组织化学荧光染色检测不同血糖浓度大鼠胃ICC数量及网络结构。结果实验组大鼠血糖浓度高于对照组,ICC数量,低于对照组,且比较差异有统计学意义(P<0.05)。实验组大鼠中血糖浓度越高,ICC数量越低,说明血糖浓度升高可能与平滑肌及神经末梢之间缝隙连接的减少及其ICC网络的超微结构损伤及异常有关。结论 DM小鼠胃组织中血糖水平的升高,可能是DM胃中ICC数量减少的原因;外源性降低血糖能改善DM相关的胃肠道ICC病变。

关键词: 血糖, 糖尿病, 胃轻瘫, 胰岛素, cajal间质细胞

Abstract: Objective To observe the effects of glucose fluctuations on Cajal interstitial cells (ICC) of rats with diabetic gastroparesis(DGP) and its mechanistic. Methods 60 Wistar rats were selected and randomly divided into two groups. 20 rats in experimental group and 40 rats in control group. Used immunofluorescence staining to detect the amount of gastric ICC and network structure in DGP rats with different glucose levels. Results The blood glucose concentration in the experimental group was significantly higher than that in the control group, the amount of ICC in the experimental group was significantly lower than that in the control group(P<0.05). The amount of ICC decreased with the increase of glucose levels. In the experimental group, The gap junctions between smooth muscle and nerve endings, ultrastructural damage and abnormalities of the ICC network were probably related to glucose level. Conclusion The increase of glucose level was probably the cause of the decrease of the amount in ICC. Exogenousy decrease glucose levels probably can help to improve the lesion of ICC with DGP.

Key words: Blood glucose, Diabetes, Gastroparesis, Insulin, Cajal interstitial cells

王艳, 黄萍, 廖淑金. 血糖波动对糖尿病胃轻瘫大鼠Cajal间质细胞的影响及其机制研究[J]. 广州医药, 2016, 47(2): 1-3.

Wang Yan, Huang Ping, Liao Shujin. Effects of blood glucose fluctuations on Cajal interstitial cells of rats with diabetic gastroparesis an its mechanistic studies[J]. Guangzhou Medical Journal, 2016, 47(2): 1-3.

参考文献

[1] 孙井军,周琦,尤承忠,等.大鼠胃空肠Roux-en-Y吻合口Cajal间质细胞再生与胃排空功能关系[J]. 中华外科杂志,2015,53(4):310-313.
[2] 黄颖,邵宇,叶道雄,等.肠道手术对肠道硫化氢生成及Cajal间质细胞破坏的影响[J]. 中华胃肠外科杂志,2015( 4):382-387.
[3] OKUMURA T,TANNO S,OHHIRA M,et al. Preva-lence of functional dyspepsia in an outpatient clinic-with primary care physicians in Japan[J]. J Gastroen-terol,2010,45(2):187-194.
[4] THOMPSON WG, HEATON KW. Functional bowel disorders in apparently healthy people[J]. Gastroenter-ology,1980,79(2):283-288.
[5] 王曙逢,仇广林,赵志浩,等.大鼠小肠Cajal间质细胞的体外分离、培养及鉴定[J]. 中国普通外科杂志,2014,23(4):494-498.
[6] 陈思伟,唐哲,刘莉,等.小儿腹泻外敷散对腹泻大鼠小肠Cajal细胞超微结构及血管活性肠肽受体1表达的影响[J]. 解放军医学杂志,2015,40(1):22-25.
[7] TALLEY NJ,VAKIL N. Guidelines for the manage-ment of dyspepsia[J]. Am J Gastroenterol,2005,100(10):2324-2337.
[8] 郭璇,谭华梁,王小娟,等.舒胃汤对功能性消化不良大鼠Cx43蛋白的分布及Cajal间质细胞的修复与再生的影响[J]. 中国中西医结合消化杂志,2014,22(11):652-657,661.
[9] 潘慧斌,陆建锋,汪瑶.Cajal间质细胞与胃肠道动力疾病的相关性研究进展[J]. 医学综述,2014,20(15):2702-2705.
[10] 尹平,徐世芬,高宁阳,等.穴位埋线对慢传输型便秘大鼠Cajal间质细胞的影响[J]. 上海针灸杂志,2014,33(6):585-588.
[11] LIN L,XU LM,ZHANG W,el aI. RoIes of stem ceIIhctor on the depIetion of interstitial cells of Caja in the colon of diabetic mice[J].Am J Phvsiol GastmintesI Liver Phvsiol,2010,298:241-247.
[12] 张磊,朱骏,朱晓亮,等.Cajal间质细胞介导急性胰腺炎引起的胃肠道动力障碍[J]. 第三军医大学学报,2014,36(15):1592-1595
[13] 韩鹃,梅峰,乔着意,等.Kit信号通路调控成年小鼠Cajal间质细胞的表型改变[J]. 第三军医大学学报,2014,36(12):1268-1272.
[14] JOHNSTON L,WOOLSEY S,CUNNINGHAM RM,et al. Morphologoical expres sion of KIT positive inIerstitial cells of CaiaJ in human bladder[J].J Urol,2010,184(1):370-377.
[15] ANDERSON UA, CARSON C, MCCLOSKEY KD. KCNO currents and their contribution to resting membrane potential and the excitabilitv of in-terstitial cells of Cajal from th8 guine8 pig bladder[J]. J Uml,2009(182):330-336.
[16] FORD AC. Eradicating Helicobacter pylori in Iunc-tional dyspepsia[J]. Gastroenterology,2012,142(7):1613-1614.
[17] 高纺,盛红梅,张田宁,等.针刺上巨虚穴对慢传输型便秘小鼠结肠组织Cajal间质细胞数的影响[J]. 安徽中医药大学学报,2014,33(3):66-69.