LINC02038与子宫内膜癌发生、发展的关联性分析

doi:10.3969/j.issn.1000-8535.2024.01.003

广州医药 ›› 2024, Vol. 55 ›› Issue (1): 18-24.DOI: 10.3969/j.issn.1000-8535.2024.01.003

LINC02038与子宫内膜癌发生、发展的关联性分析

赵二勇, 陈悦霖, 易莉莎, 林佩萱

广州市妇女儿童医疗中心（广东广州 510180）

收稿日期:2023-08-17 出版日期:2024-01-20 发布日期:2024-01-24
通讯作者: 林佩萱,E-mail：lpx20062007@126.com
作者简介:注：赵二勇与陈悦霖对本文具有同等贡献
基金资助:
广州市科技计划项目（2023A03J0870）

Association analysis of LINC02038 with endometrial carcinoma progression

ZHAO Eryong, CHEN Yuelin, YI Lisha, LIN Peixuan

Guangzhou Women and Children's Medical Center,Guangzhou 510180,China

Received:2023-08-17 Online:2024-01-20 Published:2024-01-24

摘要/Abstract

摘要： 目的分析长链非编码RNA LINC02038的表达与子宫内膜癌发生、发展的关联性,并探讨其潜在的生物学调控机制,为子宫内膜癌的精准诊治提供科学线索。方法采用荧光定量PCR技术检测LINC02038 在2019年—2020年期间于我院收集的42例子宫内膜癌标本及相应癌旁正常组织中的表达差异。构建LINC02038过表达载体并转染子宫内膜癌Ishikawa 细胞系,通过CCK-8、Transwell等功能实验验证其对肿瘤细胞增殖、侵袭能力的影响。利用TCGA公共数据库分析LINC02038与子宫内膜癌预后的相关性,并通过基因本体论（GO）、京都基因组百科全书（KEGG）及基因集富集分析（GSEA）等生物信息学方法预测其潜在的下游调控机制。结果 LINC02038在子宫内膜癌组织中的表达高于癌旁正常组织（P<0.001）。过表达LINC02038可促进子宫内膜癌细胞Ishikawa的增殖和迁移。生物信息学分析提示LINC02038可能通过调控细胞分化、激素分泌、细胞外基质重塑等过程,激活NF-κB、细胞外基质受体等信号通路影响子宫内膜癌的发生。结论 LINC02038的异常表达与子宫内膜癌的发生、发展相关联,可作为评估子宫内膜癌发病风险的候选生物标志物。

关键词: 子宫内膜癌, 长链非编码RNA, LINC02038

Abstract: Objective To analyze the expression of long non-coding RNA LINC02038 and its relationship with the occurrence and development of endometrial carcinoma（EC）,explore its potential biological mechanisms,and provide potential biomarkers for targeted therapy of EC．Methods Quantitative real-time PCR was used to detect the expression levels of LINC02038 in 42 EC tissues and their adjacent tissues．The LINC02038 overexpressin vector was constructed and transfected into EC Ishikawa cells．CCK-8,Transwell migration and invasion assays were performed to examine the effects of LINC02038 overexpression on cancer cell proliferation,migration and invasion．Public TCGA data were analyzed to investigate the associations between LINC02038 and EC pathogenesis and prognosis．GO,KEGG and GSEA enrichment analyses were conducted to elucidate the potential biological mechanisms of LINC02038．Results LINC02038 expression was significantly upregulated in EC tissues compared to adjacent non-tumor tissues（P<0.001）．Overexpression of LINC02038 markedly promoted the proliferation and migration of Ishikawa cells．Bioinformatics analysis suggested that LINC02038 may participate in regulating cell differentiation,hormone secretion,extracellular matrix remodeling and other processes,as well as activating NF-κB,extracellular matrix receptor and other signaling pathways involved in endometrial carcinogenesis．Conclusions The aberrant expression of LINC02038 is associated with EC occurrence and may serve as a potential biomarker for assessing the risk of this cancer．

Key words: endometrial carcinoma, long non-coding RNA, LINC02038

赵二勇, 陈悦霖, 易莉莎, 林佩萱. LINC02038与子宫内膜癌发生、发展的关联性分析[J]. 广州医药, 2024, 55(1): 18-24.

ZHAO Eryong, CHEN Yuelin, YI Lisha, LIN Peixuan. Association analysis of LINC02038 with endometrial carcinoma progression[J]. Guangzhou Medical Journal, 2024, 55(1): 18-24.

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LINC02038与子宫内膜癌发生、发展的关联性分析

Association analysis of LINC02038 with endometrial carcinoma progression

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