叶酸下调子宫内膜癌中NRBP2基因的表达

doi:10.3969/j.issn.1000-8535.2020.06.004

广州医药 ›› 2020, Vol. 51 ›› Issue (6): 19-23.DOI: 10.3969/j.issn.1000-8535.2020.06.004

叶酸下调子宫内膜癌中NRBP2基因的表达

朱婷婷¹, 何淑明²

1 南方医科大学第二临床医学院(广州 510515)
2 南方医科大学附属小榄医院(中山 528415)

收稿日期:2020-02-24 出版日期:2020-11-20 发布日期:2021-11-28
通讯作者: 何淑明,E-mail:13703036288@139.com
基金资助:
中山市社会公益科技研究项目重大专项(2018B1001)

Folic acid down-regulates NRBP2 gene expression in endometrial cancer

ZHU Tingting¹, HE Shuming²

1 The Second School of Clinical Medicine,Southern Medical University,Guangzhou 510515,China
2 Affiliated Xiaolan Hospital,Southern Medical University,Zhongshan 528415,China

Received:2020-02-24 Online:2020-11-20 Published:2021-11-28

摘要/Abstract

摘要： 目的探究叶酸对子宫内膜癌作用的靶基因。方法通过转录组测序筛选叶酸作用下子宫内膜癌细胞中的差异基因,生存分析寻找对子宫内膜癌具有生存意义的差异基因,qPCR及western blot检测其在叶酸作用下的表达。结果转录组测序发现36个差异基因,生存分析发现FMN1,TRIB3,INHBE及NRBP2的表达对子宫内膜癌具有生存意义,qPCR及western blot验证叶酸作用下NRBP2在子宫内膜癌细胞中的表达下调。结论叶酸下调子宫内膜癌中NRBP2基因的表达,NRBP2可能是叶酸对子宫内膜癌作用的靶标。

关键词: 子宫内膜癌, 叶酸, 转录组测序

Abstract: Objective To explore the target genes of folic acid on endometrial carcinoma. Methods The differential genes in endometrial cancer cells treated with folic acid were screened by transcriptome sequencing. Survival analysis was used to find the differential genes with survival significance. QPCR and western blot were used to detect their expression under the action of folic acid. Results 36 differential genes were found by transcriptional sequencing. Survival analysis showed that the expression of FMN1,TRIB3,INHBE and NRBP2 had survival significance in endometrial carcinoma. QPCR and western blot confirmed that the expression of NRBP2 in endometrial cancer cells was down-regulated by folic acid. Conclusion Folic acid down-regulates the expression of NRBP2 gene in endometrial carcinoma, and NRBP2 may be the target of the effect of folic acid on endometrial carcinoma.

Key words: Endometrial cancer, Folic acid, Transcriptome sequencing

朱婷婷, 何淑明. 叶酸下调子宫内膜癌中NRBP2基因的表达[J]. 广州医药, 2020, 51(6): 19-23.

ZHU Tingting, HE Shuming. Folic acid down-regulates NRBP2 gene expression in endometrial cancer[J]. Guangzhou Medical Journal, 2020, 51(6): 19-23.

叶酸下调子宫内膜癌中NRBP2基因的表达

Folic acid down-regulates NRBP2 gene expression in endometrial cancer

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