TIMELESS、RAB2A、ASPM在乳腺癌组织中的表达及与临床特征相关性

doi:10.3969/j.issn.1000-8535.2023.05.014

摘要/Abstract

摘要： 目的分析TIMELESS、鼠肉瘤病毒家族相关蛋白2A（RAB2A）、异常纺锤体样小头畸形相关基因（ASPM）在乳腺癌组织中的表达及与临床特征相关性。方法选取2019年2月—2021年2月我院乳腺癌组织标本84例作为研究组、正常乳腺组织标本53例作为对照组,采用荧光定量聚合酶测定TIMELESS、ASPM,采用Western blot检测RAB2A蛋白表达情况,分析上述三个指标在乳腺癌中表达及与临床特征相关性。结果对比对照组,研究组TIMELESS、ASPM表达较高,RAB2A较低（P<0.05）。TIMELESS、RAB2A、ASPM与乳腺癌淋巴结浸润、TNM分期、分化程度相关（P<0.05）。TIMELESS、RAB2A、ASPM为影响乳腺癌发生的危险因素（P<0.05）。TIMELESS、RAB2A负相关（r=-0.383、P=0.001）;TIMELESS、ASPM正相关（r=0.397、P=0.001）;RAB2A、ASPM负相关（r=-0.257、P=0.018）。对比TIMELESS、RAB2A、ASPM单一检测,三者联合检测对乳腺癌的诊断价值较高（P<0.05）。结论乳腺癌患者TIMELESS、ASPM呈高表达,RAB2A呈低表达,上述三个指标与乳腺癌高度相关,可作为乳腺癌发生的检测指标。

关键词: TIMELESS, 异常纺锤体样小头畸形相关基因, 乳腺癌, 临床特征

Abstract: Objective To analyze the expression of TIMELESS,murine sarcoma virus family related protein 2A（RAB2A）and abnormal spindle like microcephaly related gene（ASPM）in breast cancer tissues and their correlation with clinical features．Methods A total of 84 breast cancer tissue samples from our hospital from February 2019 to February 2021 were selected as the study group and 53 normal breast tissue samples were selected as the control group．Time,ASPM and RAB2A protein expression were determined by fluorescent quantitative polymerase,and RAB2A protein expression was detected by Western blot．The expression of the above three indicators in breast cancer and their correlation with clinical characteristics were analyzed．Results Compared with the control group,the study group had higher TIMELESS and ASPM expression levels and lower RAB2A level（P<0.05）．TIMELESS,RAB2A and ASPM expressions were correlated with lymph node infiltration,TNM stage and differentiation of breast cancer（P<0.05）．TIMELESS,RAB2A and ASPM were the risk factors of breast cancer（P<0.05）．TIMELESS and RAB2A were negatively correlated（r=-0.383,P=0.001）;TIMELESS and ASPM were positive correlated（r=0.397、P=0.001）;RAB2A and ASPM were negatively correlated（r=-0.257,P=0.018）．Compared with the single detection of TIMELESS,RAB2A and ASPM,the combined detection had higher diagnostic value for breast cancer（P<0.05）．Conclusions Patients with breast cancer had high expression of TIMELESS and ASPM,and low expression of RAB2A．The above three indicators were highly correlated with breast cancer and can be detection indicators for breast cancer．

Key words: TIMELESS, abnormal spindle like microcephaly related genes, breast cancer, clinical features

刘起鹏, 张婷, 郭婉莹. TIMELESS、RAB2A、ASPM在乳腺癌组织中的表达及与临床特征相关性[J]. 广州医药, 2023, 54(5): 80-86.

LIU Qipeng, ZHANG Ting, GUO Wanying. Expression of TIMELESS,RAB2A and ASPM in breast cancer and their correlation with clinical features[J]. Guangzhou Medical Journal, 2023, 54(5): 80-86.

参考文献

[1] TAURIN S,ALKHALIFA H.Breast cancers,mammary stem cells,and cancer stem cells,characteristics,and hypotheses[J].Neoplasia,2020,22(12):663-678.
[2] KIM T M,YANG I S,SEUNG B J,et al.Cross-species oncogenic signatures of breast cancer in canine mammary tumors[J].Nat Commun,2020,11(1):3616.
[3] LE NAOUR A,ROSSARY A,VASSON M P.EO771,is it a well-characterized cell line for mouse mammary cancer model? Limit and uncertainty[J].Cancer Med,2020,9(21):8074-8085.
[4] WHITE J.Benefit vs harm of internal mammary node irradiation for node- positive breast cancer[J].J Natl Cancer Inst,2021,113(10):1277-1278.
[5] TOCCI J M,FELCHER C M,GARCÍA SOLÁ M E,et al.R-spondin-mediated WNT signaling potentiation in mammary and breast cancer development[J].IUBMB Life,2020,72(8):1546-1559.
[6] 中国女医师协会临床肿瘤学专业委员会,中国抗癌协会乳腺癌专业委员会.中国进展期乳腺癌共识指南(CABC2015)[J].癌症进展,2015(3):223-245.
[7] HASHEM T,ABDELMOEZ A,ROZEKA A M,et al.Intra-mammary lymph nodes,an overlooked breast cancer prognostic tool[J].World J Surg Oncol,2021,19(1):114.
[8] LEDET M M,HARMAN R M,FAN J C,et al.Secreted sphingomyelins modulate low mammary cancer incidence observed in certain mammals[J].Sci Rep,2020,10(1):20580.
[9] HILLERS-ZIEMER L E,ARENDT L M.Weighing the risk:effects of obesity on the mammary gland and breast cancer risk[J].J Mammary Gland Biol Neoplasia,2020,25(2):115-131.
[10] ATTALLA S,TAIFOUR T,BUI T,et al.Insights from transgenic mouse models of PyMT-induced breast cancer:recapitulating human breast cancer progression in vivo[J].Oncogene,2021,40(3):475-491.
[11] FU N Y,NOLAN E,LINDEMAN G J,et al.Stem cells and the differentiation hierarchy in mammary gland development[J].Physiol Rev,2020,100(2):489-523.
[12] 粟连秀,陈静平,杨达平,等.沉默生物钟基因Timeless对卵巢癌SKOV3细胞凋亡和侵袭能力的影响[J].中国病理生理杂志,2019,35(7):1169-1175.
[13] 王静松,张建淮,孙虎.生物节律基因Timeless表达与不同部位直肠癌预后的关系[J].中华普通外科学文献(电子版),2019,13(5):350-353.
[14] 李淑晶,任薛莉,伍会健.生物钟蛋白TIMELESS对乳腺癌细胞增殖和侵袭转移的差异调控[J].中国生物化学与分子生物学报,2020,36(2):207-216.
[15] TOUMAZI D,EL DACCACHE S,CONSTANTINOU C.An unexpected link:The role of mammary and gut microbiota on breast cancer development and management(Review)[J].Oncol Rep,2021,45(5):80.
[16] 黄国定,卢宏全,潘敏丽,等.鼠肉瘤病毒家族相关蛋白2A对胃癌细胞增殖与侵袭和迁移能力的影响[J].中国临床药理学杂志,2021,37(23):3244-3248.
[17] CHARIFOU E,TRAUSTADOTTIR GA,BENTIRES-ALJ M,et al.Twelfth Annual ENBDC Workshop:methods in mammary gland biology and breast cancer[J].J Mammary Gland Biol Neoplasia,2021,26(3):221-226.
[18] 季家祥,赵扬,齐晓杰,等.ASPM在肝癌中的表达及其与肝癌肝移植预后的关系[J].中华器官移植杂志,2021,42(7):408-412.
[19] 王俊杰,何振宇,段仁慧.ASPM在肺腺癌中的表达及其与肺腺癌的进展和预后关系的研究[J].中国肺癌杂志,2020,23(1):29-35.
[20] 安晓静,柏苗苗,谢振年,等.结肠癌中ASPM对细胞增殖、侵袭和凋亡的影响[J].临床与实验病理学杂志,2021,37(10):1177-1182.