吴茱萸碱通过阻滞细胞周期而抑制人骨肉瘤细胞增殖

doi:10.3969/j.issn.1000-8535.2020.02.003

广州医药 ›› 2020, Vol. 51 ›› Issue (2): 10-14.DOI: 10.3969/j.issn.1000-8535.2020.02.003

吴茱萸碱通过阻滞细胞周期而抑制人骨肉瘤细胞增殖

徐绘华¹, 苏文洲¹, 陈存特¹, 黄千峰², 黄智通¹, 郭奇峰¹

1 广州市第一人民医院(广州 510180)
2 广东省妇幼保健院(广州 511400)

收稿日期:2019-11-01 出版日期:2020-03-20 发布日期:2021-11-28
通讯作者: 郭奇峰,E-mail:guoqifenggz@163.com
基金资助:
国家自然科学基金资助项目(31601031)

Evodiamine inhibits proliferation of human osteosarcoma cells by arresting the cell cycle

XU Huihua¹, SU Wenzhou¹, CHEN Cunte¹, HUANG Qianfeng², HUANG Zhitong¹, GUO Qifeng¹

1 Guangzhou First People's Hospital, Guangzhou 510180,China
2 Guangdong Women and Children Hospital, Guangzhou 511400,China

Received:2019-11-01 Online:2020-03-20 Published:2021-11-28

摘要/Abstract

摘要： 目的体外细胞实验检测吴茱萸碱对骨肉瘤HOS细胞株细胞周期及体外增殖能力的影响。方法通过利用浓度为0、3、6、12 μmol/L吴茱萸碱处理骨肉瘤HOS细胞48 h后,Hoechst-33258荧光染色观察不同浓度吴茱萸碱处理后HOS细胞核的形态学变化。利用流式细胞术检测3 μmol/L的吴茱萸碱处理后骨肉瘤HOS细胞的细胞周期分布变化。结果 3、6、12 μmol/L的骨肉瘤吴茱萸碱处理细胞,细胞呈现凋亡核碎裂等典型变化,而且随药物剂量增加而更趋明显。呈剂量依赖性抑制其体外增殖能力。3 μmol/L吴茱萸碱处理骨肉瘤HOS细胞0、24、48、72 h,各组细胞周期变化如下:G0/G1期:对照组(51.12±2.13)%、24 h(19.17±1.02)%、48 h(16.94±1.67)%、72 h(11.05±1.25)%;S期:对照组(32.92±0.73)%、24 h(31.00±1.42)%、48 h(32.38±3.03)%、72 h(29.18±2.87)%;G2/M期:对照组(16.01±2.26)%、24 h(49.82±0.62)%、48 h(50.6767±2.80)%、72 h(59.56±1.97)%。结论吴茱萸碱可诱导人骨肉瘤HOS细胞发生G2/M期阻滞,而S期变化不明显。说明吴茱萸碱可以抑制骨肉瘤细胞的增殖能力,并阻滞细胞周期于G2/M期。

关键词: 吴茱萸碱, 人骨肉瘤HOS细胞, 增殖, 细胞周期

Abstract: Objective Using transcriptome sequencing and in vitro cell assay to detect the effect of evodiamine on cell cycle and proliferation in osteosarcoma HOS cell line. Methods HOS cells were treated with evodiamine at 0, 3, 6, and 12 μmol/L for 48 hours, Hoechst-33258 fluorescence staining was used to observe the morphological changes of HOS nuclei after treatment with different concentrations of evodiamine.The cell cycle distribution of HOS cells treated with 3 μmol/L evodiamine was detected by flow cytometry. Results 3,6,12 μmol/L osteosarcoma treated with evodiamine, the cells showed typical changes such as apoptotic nuclear fragmentation, and it became more obvious with the increase of drug dosage. Inhibition of proliferation in vitro in a dose-dependent manner.HOS cells were treated with 3 μmol/L evodiamine for 0, 24, 48, 72 h. The cell cycle changes of each group were as follows: G0/G1 phase: control group(51.12±2.13)%, 24 h(19.17±1.02)%, 48 h(16.94±1.67) %, 72 h(11.05±1.25)%;S phase: control group(32.92±0.73)%, 24 h(31.00±1.42)%, 48 h(32.38±3.03)%, 72 h(29.18±2.87)%;G2/M period: control group(16.01±2.26)%, 24 h(49.82±0.62)%, 48 h(50.6767±2.80)%, 72 h(59.56±1.97)%. Conclusion Analysis of the above results revealed that evodiamine can induce G2/M phase arrest in human osteosarcoma HOS cells, but the S phase changes are not obvious. It indicated that evodiamine would inhibit the proliferation of osteosarcoma cells and block the cell cycle in G2/M phase.

Key words: Evodiamine, Human osteosarcoma HOS cells, Proliferation, Cell cycle

徐绘华, 苏文洲, 陈存特, 黄千峰, 黄智通, 郭奇峰. 吴茱萸碱通过阻滞细胞周期而抑制人骨肉瘤细胞增殖[J]. 广州医药, 2020, 51(2): 10-14.

XU Huihua, SU Wenzhou, CHEN Cunte, HUANG Qianfeng, HUANG Zhitong, GUO Qifeng. Evodiamine inhibits proliferation of human osteosarcoma cells by arresting the cell cycle[J]. Guangzhou Medical Journal, 2020, 51(2): 10-14.

参考文献

[1] DORFMAN H D, CZERNIAK B. Bone cancers[J]. Cancer, 2015(75):203-210.
[2] KAGER L, ZOUBEK A, DOMINKUS M, et al. Osteosarcoma in very young children: experience of the Cooperative Osteosarcoma Study Group[J]. Cancer, 2010, 116(22):5316-5324.
[3] MIRABELLO L, TROISI R J, SAVAGE S A. International osteosarcoma incidence patterns in children and adolescents, middle ages and elderly persons[J]. Int J Cancer, 2010, 125(1):229-234.
[4] OTTAVIANI G, JAFFE N. The etiology of osteosarcoma[J]. Cancer Treat Res, 2009, 152(152):15-32.
[5] DHAMMI I K, KUMAR S. Osteosarcoma: A journey from amputation to limb salvage[J]. Indian J Orthop, 2014, 48(3):233-234.
[6] MEYERS P A, SCHWARTZ C L, KRAILO M D, et al. Osteosarcoma: the addition of muramyl tripeptide to chemotherapy improves overall survival--a report from the Children's Oncology Group[J]. J Clin Oncol, 2015, 26(4):633-638.
[7] WHELAN J, PATTERSON D, PERISOGLOU M, et al. The role of interferons in the treatment of osteosarcoma[J]. Pediatr Blood Cancer, 2010, 54(3):350-354.
[8] 张涵妮,祝文浩,王慧茹,等. 吴茱萸碱对人胃癌BGC-823细胞增殖的影响及分子机制研究[J]. 亚太传统医药,2019(4):19-23.
[9] 周鹏,吕艳伟,李静,等. 吴茱萸碱通过NF-κB-p65信号通路抑制人结肠癌细胞SW480的迁移和侵袭[J]. 第三军医大学学报,2019,41(6):549-555.
[10] HONG J Y,PARK S H,MIN H Y,et al. Anti-proliferative effects of evodiamine in human lung cancer cells[J]. J Cancer Prev,2014,19(1):7-13.
[11] 徐俊杰,杨然,杨芳景,等. 吴茱萸碱抗肿瘤机制的研究进展[J]. 上海交通大学学报,2018,38(5):578-583.
[12] 吕艳伟,郭星娴,周鹏,等. 吴茱萸碱结肠癌细胞自噬抑制其增殖的研究[J]. 中草药,2018(20):4851-4856.
[13] KAN S F, YU C H, PU H F, et al. Anti-proliferative effects of evodiamine on human prostate cancer cell lines DU145 and PC3[J]. J Cell Biochem,2007(101):44-56.
[14] LIAO C H, PAN S L, GUH J H, et al. Antitumor mechanism of evodiamine, a constituent from Chinese herb Evodiae fructus, in human multiple-drug resistant breast cancer NCI/ADR-RES cells in vitro and in vivo[J]. Carcinogenesis,2005,26(5):968-975.
[15] 张庆然,周昭伶,潘振海,等. 吴茱萸碱抑制Huh7细胞生长并增强细胞对TRAIL的敏感性[J]. 中国病理生理杂志,2018,34(2) :212-217.

吴茱萸碱通过阻滞细胞周期而抑制人骨肉瘤细胞增殖

Evodiamine inhibits proliferation of human osteosarcoma cells by arresting the cell cycle

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价

[1]	郎吉萍, 戴秋月, 吕萍, 郭志刚. 阿司匹林抗结直肠癌的作用机制的生物信息学分析[J]. 广州医药, 2021, 52(6): 23-34.
[2]	黄千峰, 武丽, 欧燕兰, 艾君, 张秀. 细胞毒素-1对人卵巢癌细胞体外增殖、凋亡的影响[J]. 广州医药, 2020, 51(6): 24-27.