低出生体重早产儿应用抗生素后的肠道菌群动态变化

doi:10.3969/j.issn.1000-8535.2021.03.013

广州医药 ›› 2021, Vol. 52 ›› Issue (3): 61-67.DOI: 10.3969/j.issn.1000-8535.2021.03.013

低出生体重早产儿应用抗生素后的肠道菌群动态变化

罗梅娟, 胡家奇, 魏颖, 欧巧群, 王丽娜, 张又祥

广州市第一人民医院,华南理工大学医学院附属第二医院儿科(广州 510180)

收稿日期:2021-01-25 发布日期:2021-11-24
通讯作者: 张又祥,E-mail: eyzhangyouxiang@scut.edu.cn
基金资助:
广州市卫生计生科技一般引导项目(2017A011244)

Dynamic changes of gut microbiota in low birth weight preterm infants after antibiotics therapy

LUO Meijuan, HU Jiaqi, WEI Ying, OU Qiaoqun, WANG Lina, ZHANG Youxiang

Department of Pediatrics, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou 510180,China

Received:2021-01-25 Published:2021-11-24

摘要/Abstract

摘要： 目的观察低出生体重早产儿应用抗生素后肠道菌群的动态变化。方法选取2018年6月—2019年7月在广州市第一人民医院住院的10名低出生体重早产儿,在出生时、出生后1~2周、出生后2~3周、出生后3~4周、出生后4~5周、出生后5~6周时分别收集粪便样本,通过16s高通量测序检测患儿粪便菌群变化并统计分析。结果应用抗生素后的低出生体重早产儿肠道菌群α多样性(Shannon指数、Simpson指数、ACE指数和PD_whole_tree指数)下降(P均<0.05),肠道菌群结构在门、科、属水平均发生改变,其中Alistipes、Bacteroides、Lactobacillus、unidentified_Lachnospiraceae、unidentified_Ruminococcaceae、Alloprevotella、unidentified_Cyanobacteria、Bacillus、Stenotrophomonas和Acinetobacter菌属相对丰度减少(P均<0.05)。结论低出生体重早产儿应用抗生素后肠道菌群多样性下降,肠道菌群结构发生改变,并在抗生素停用后仍持续,针对性补充益生菌或益生元可能有助于肠道菌群恢复稳态。

关键词: 低出生体重, 早产儿, 肠道菌群, 抗生素, 高通量测序

Abstract: Objective To observe the dynamic changes of gut microbiota in premature infants with low birth weight after antibiotics therapy. Methods 10 low birth weight premature infants hospitalized in Guangzhou First People's Hospital from June 2018 to July 2019 were included. Fecal samples were collected at birth, 1~2 weeks after birth, 2~3 weeks after birth, 3~4 weeks after birth, 4~5 weeks after birth and 5~6 weeks after birth, respectively. The changes of fecal microbiota were detected and analyzed by 16s high-throughput sequencing. Results The α-diversity of gut microbiota (Shannon index, Simpson index, ACE index and PD_whole_tree index) in low birth weight preterm infants treated with antibiotics decreased (P<0.05). The structure of gut microbiota changed at phylum, family and genus levels, among which Alistipes, Bacteroides, Lactobacillus, unidentified_Lachnospiraceae, unidentified_Ruminococcaceae, Alloprevotella, unidentified_Cyanobacteria, Bacillus, Stenotrophomonas and Acinetobacter decreased (P<0.05). Conclusion The diversity of gut microbiota in low birth weight preterm infants decreased and the structure of gut microbiota changed after antibiotic therapy. Targeted supplementation of probiotics or prebiotics may contribute to the recovery of gut microbial homeostasis.

Key words: Low birth weight, Premature infants, Gut microbiota, Antibiotics, High-throughput sequencing

罗梅娟, 胡家奇, 魏颖, 欧巧群, 王丽娜, 张又祥. 低出生体重早产儿应用抗生素后的肠道菌群动态变化[J]. 广州医药, 2021, 52(3): 61-67.

LUO Meijuan, HU Jiaqi, WEI Ying, OU Qiaoqun, WANG Lina, ZHANG Youxiang. Dynamic changes of gut microbiota in low birth weight preterm infants after antibiotics therapy[J]. Guangzhou Medical Journal, 2021, 52(3): 61-67.

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低出生体重早产儿应用抗生素后的肠道菌群动态变化

Dynamic changes of gut microbiota in low birth weight preterm infants after antibiotics therapy

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