Wnt-1信号通路蛋白-3（WISP-3）在高氧诱导肺上皮细胞凋亡中的保护作用

doi:10.3969/j.issn.1000-8535.2018.06.002

广州医药 ›› 2018, Vol. 49 ›› Issue (6): 6-12.DOI: 10.3969/j.issn.1000-8535.2018.06.002

Wnt-1信号通路蛋白-3（WISP-3）在高氧诱导肺上皮细胞凋亡中的保护作用

魏树全, 王康玮, 黄晓梅, 唐万娜, 赵祝香, 赵子文

广州市第一人民医院,华南理工大学附属第二医院,呼吸与危重症医学科（广州 510180）

收稿日期:2018-08-04 出版日期:2018-11-20 发布日期:2021-11-30
通讯作者: 赵子文,E-mail：zhaozw2016@163.com
基金资助:
广东省医学科学技术研究基金项目（A2015385）

The protective effect of Wnt-1 inducible signaling pathway protein-3 （WISP-3） in hyperoxia-induced apoptosis in lung epithelial Cells

WEI Shuquan, WANG Kangwei, HUANG Xiaomei, TANG Wanna, ZHAO Zhuxiang, ZHAO Ziwen

The Department of Pulmonary and Critical Care Medicine,Guangzhou First People's Hospital,The Second Affiliated Hospital of South China University of Technology,Guangzhou 510180 ,China

Received:2018-08-04 Online:2018-11-20 Published:2021-11-30

摘要/Abstract

摘要： 目的在原来研究的基础上进一步研究Wnt-1信号通路蛋白-3（WISP-3）在高氧诱导肺上皮细胞凋亡中的作用。方法通过Western blot检测和免疫组化检测不同肺上皮细胞中WISP-3的蛋白表达量。利用质粒转染和siRNA的方法在Beas-2B细胞中高表达和基因沉默WISP-3,通过细胞活性检测和流式细胞学技术检测高氧刺激后细胞的凋亡情况。结果与空气对照相比,高氧刺激使肺上皮细胞的WISP-3蛋白表达量下降;WISP-3基因沉默或高表达使高氧诱导的肺上皮细胞凋亡增加或减少。结论高氧刺激下,肺上皮细胞中WISP-3表达下降,WISP-3对高氧诱导的肺上皮细胞凋亡具有保护作用。

关键词: 高氧, 细胞凋亡, WISP-3, ALI/ARDS

Abstract: Objective To explore how Wnt-1 inducible signaling pathway protein-3 （WISP-3） participate in and play a regulatory role in the process of hyperoxia induced apoptosis in lung epithelial cells. Methods The expression of WISP-3 was detected via Western blot and immunohistochemistry. High expression and low expression of WISP-3 were performed by plasmid transfection and siRNA. Cell viability and flow cytometry were executed to detect the hyperoxia-induced apoptosis in Beas-2B. Results Compared to the group of air control,the expression of WISP-3 protein in lung epithelial cells decreased obviously after hyperoxia. Cell survival decrease and apoptosis increased after hyperoxia in Beas-2B cells with low expression of WISP-3. Vice versa. Conclusion The expression of WISP-3 decreased after hyperoxia in lung epithelial cells. The role of WISP-3 in this process may be protective.

Key words: Hyperoxia, Apoptosis, WISP-3, ALI/ARDS

魏树全, 王康玮, 黄晓梅, 唐万娜, 赵祝香, 赵子文. Wnt-1信号通路蛋白-3（WISP-3）在高氧诱导肺上皮细胞凋亡中的保护作用[J]. 广州医药, 2018, 49(6): 6-12.

WEI Shuquan, WANG Kangwei, HUANG Xiaomei, TANG Wanna, ZHAO Zhuxiang, ZHAO Ziwen. The protective effect of Wnt-1 inducible signaling pathway protein-3 （WISP-3） in hyperoxia-induced apoptosis in lung epithelial Cells[J]. Guangzhou Medical Journal, 2018, 49(6): 6-12.

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Wnt-1信号通路蛋白-3（WISP-3）在高氧诱导肺上皮细胞凋亡中的保护作用

The protective effect of Wnt-1 inducible signaling pathway protein-3 （WISP-3） in hyperoxia-induced apoptosis in lung epithelial Cells

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