复发性流产男性精浆弹性蛋白酶与精液参数及DNA碎片率的相关性分析

doi:10.3969/j.issn.1000-8535.2020.05.009

广州医药 ›› 2020, Vol. 51 ›› Issue (5): 41-45.DOI: 10.3969/j.issn.1000-8535.2020.05.009

复发性流产男性精浆弹性蛋白酶与精液参数及DNA碎片率的相关性分析

凌晓辉¹, 陈佳鸿², 陈志云¹, 朱善文¹, 刘利敏¹, 陈光乐¹, 柯洁荣¹

1 惠州市中心人民医院生殖中心(惠州 516001)
2 惠州市中心人民医院泌尿外科 (惠州 516001)

收稿日期:2020-03-29 出版日期:2020-09-20 发布日期:2021-11-28
通讯作者: 凌晓辉,E-mail: lxhui87@hotmail.com
基金资助:
惠州市科技计划项目(190409094571998);广东省自然科学基金项目(2017A030310593)

The correlative analysis of seminal elastase on semen parameters and DNA fragmentation in the male patients of recurrent pregnancy loss

LING Xiaohui¹, CHEN Jiahong², CHEN Zhiyun¹, ZHU Shanwen¹, LIU Limin¹, CHEN Guangle¹, KE Jierong¹

1 Reproductive Medicine Centre, Huizhou Central People's Hospital, Guangdong Medical University,Huizhou 516001, China
2 Department of Urology, Huizhou Central People's Hospital, Guangdong Medical University, Huizhou 516001, China

Received:2020-03-29 Online:2020-09-20 Published:2021-11-28

摘要/Abstract

摘要： 目的我们探讨2019年6月—2020年1月复发性流产夫妇男性患者精浆弹性蛋白酶同精液参数及DNA碎片率的可能关系。方法研究对象纳入80例复发性流产的男性患者及25例因女方输卵管因素行IVF-ET正常生育的男性患者。精液标本用来进行精浆弹性蛋白酶、精液常规分析、精子核染色质分析及精子形态学等参数分析。结果结果表明同正常生育男性相比,复发性流产的弹性蛋白酶是增高(P=0.010)。我们将复发性流产男性患者分为正常组(<600 ng/mL)及异常组(≥600 ng/mL)。结果表明异常组患者的精子前向运动比例(P=0.002)及正常形态百分率(P=0.009)均降低,而精子DNA碎片率(P=0.002)增高。Spearman相关性分析发现精浆弹性蛋白酶同精子前向运动比例(r=-0.43,P<0.001)及正常形态百分率(r=-0.39,P<0.001)负相关,而同精子DNA碎片率(r=0.36,P=0.001)正相关。结论精浆弹性蛋白酶可能影响复发性流产男性患者的精子活力、形态及DNA碎片率。复发性流产男性患者的生殖道隐性感染值得重视,其相关临床探讨性值得深入研究。

关键词: 复发性流产, 精浆弹性蛋白酶, 精子DNA碎片率, 精液参数

Abstract: Objective Our study is aim to investigate the possible relationship of seminal elastase, on semen parameters and DNA fragmentation in male patients of recurrent pregnancy loss (RPL) between June 2019 and January 2020. Methods The patients included 80 male patients of RPL couple and 25 male patients from couples with clinical pregnancy through in vitro fertilization due to the female tubal factor. The semen samples were used to determine the seminal elastase, computed assisted semen analysis, sperm dispersion test and sperm morphology analysis. Results Compared to the control group, the levels of seminal elastase was increased in the RPL group. The RPL group was divided into the normal group (Elastase<600 ng/mL) and abnormal group (Elastase ≤ 600 ng/mL).The abnormal group exhibited the lower percentage of progressive sperm (P=0.002) and normal morphology (P=0.009),but higher precentage of DNA fragmentation (P=0.002). Meanwhile, the seminal elastase was positively associated with DNA fragmentation (r=0.36,P=0.001), but was inversely associated with the sperm motility (r=-0.43,P<0.001) and normal morphology (r=-0.39,P<0.001). Conclusion Our study may unveil the possible effects of the seminal elastase on the semen parameters and DNA fragmentation in the male patients of RPL couples. Further studies should put more emphasis on the silent genital tract inflammation of the patients.

Key words: Recurrent pregnancy loss, Elastase, DNA fragmentation, Semen parameters

凌晓辉, 陈佳鸿, 陈志云, 朱善文, 刘利敏, 陈光乐, 柯洁荣. 复发性流产男性精浆弹性蛋白酶与精液参数及DNA碎片率的相关性分析[J]. 广州医药, 2020, 51(5): 41-45.

LING Xiaohui, CHEN Jiahong, CHEN Zhiyun, ZHU Shanwen, LIU Limin, CHEN Guangle, KE Jierong. The correlative analysis of seminal elastase on semen parameters and DNA fragmentation in the male patients of recurrent pregnancy loss[J]. Guangzhou Medical Journal, 2020, 51(5): 41-45.

参考文献

[1] HONG LI Y, MARREN A. Recurrent pregnancy loss: a summary of international evidence-based guidelines and practice [J].Aust J Gen Pract, 2018,47(7):432-436.
[2] PUSCHECK E E, JEYENDRAN R S.The impact of male factor on recurrent pregnancy loss [J].Curr Opin Obstet Gynecol, 2007,19(3):222-228.
[3] OSMAN A, ALSOMAIT H, SESHADRI S, et al. The effect of sperm DNA fragmentation on live birth rate after IVF or ICSI: a systematic review and meta-analysis [J].Reprod Biomed Online, 2015,30(2):120-127.
[4] ALVAREZ SEDó C1, BILINSKI M, LORENZI D, et al.Effect of sperm DNA fragmentation on embryo development: clinical and biological aspects [J].JBRA Assist Reprod, 2017,21(4):343-350.
[5] BAGHERI S, ROGHANIAN R, GOLBANG N, et al. Molecular evidence of chlamydia trachomatis infection and its relation to miscarriage [J]. Int J Fertil Steril, 2018,12(2):152-156.
[6] SOLOMON M, HENKEL R. Semen culture and the assessment of genitourinary tract infections [J]. Indian J Urol, 2017,33(3):188-193.
[7] KOPA Z, WENZEL J, PAPP G K, et al.Role of granulocyte elastase and interleukin-6 in the diagnosis of male genital tract inflammation [J].Andrologia, 2005,37(5):188-194.
[8] 陆金春.精液生化指标的全自动检测及临床应用[J].中华男科学杂志, 2018,24(4): 291-296.
[9] 陈浩, 李梦颖, 许珊丹, 等. 前列腺按摩液/精浆弹性蛋白酶联合诊断ⅢA型前列腺炎合并不育症的价值[J]. 中华男科学杂志,2017,23(9):786-792.
[10] VIRRO M R, LARSON-COOK K L, EVENSON D P.Sperm chromatin structure assay (SCSA) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles [J]. Fertil Steril, 2004, 81(5):1289-1295.
[11] MCQUEEN D B, ZHANG J, ROBINS J C.Sperm DNA fragmentation and recurrent pregnancy loss: a systematic review and meta-analysis [J].Fertil Steril, 2019, 112(1):54-60.e3.
[12] IBRAHIM Y, JOHNSTONE E.The male contribution to recurrent pregnancy loss [J]. Transl Androl Urol, 2018, 7(Suppl 3):S317-S327.
[13] MORIYAMA A, SHIMOYA K, KAWAMOTO A, et al. Secretory leukocyte protease inhibitor (SLP) concentrations in seminal plasma: SLPI restores sperm motility reduced by elastase [J].Mol Hum Reprod, 1998, 4(10):946-950.
[14] KAMKAR N, RAMEZANALI F, SABBAGHIAN M. The relationship between sperm DNA fragmentation, free radicals and antioxidant capacity with idiopathic repeated pregnancy loss [J].Reprod Biol, 2018, 18(4):330-335.
[15] IBRAHIM Y, JOHNSTONE E.The male contribution to recurrent pregnancy loss [J]. Transl Androl Urol,2018, 7(Suppl 3):S317-S327.
[16] ZORN B, IHAN A, KOPITAR A N, et al.Changes in sperm apoptotic markers as related to seminal leukocytes and elastase [J]. Reprod Biomed Online, 2010, 21(1):84-92.
[17] 郑慧澜, 袁启龙, 何君伟,等.不育男性精浆弹性蛋白酶水平与精液主要参数及精子DNA完整性的关系分析[J].热带医学杂志, 2019,19(2): 145-147,169.

复发性流产男性精浆弹性蛋白酶与精液参数及DNA碎片率的相关性分析

The correlative analysis of seminal elastase on semen parameters and DNA fragmentation in the male patients of recurrent pregnancy loss

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