献血者HBV核酸检测非重复反应性确认及追踪结果分析

doi:10.3969/j.issn.1000-8535.2021.05.018

广州医药 ›› 2021, Vol. 52 ›› Issue (5): 83-87.DOI: 10.3969/j.issn.1000-8535.2021.05.018

献血者HBV核酸检测非重复反应性确认及追踪结果分析

高磊¹, 赵梅², 高文杰¹

1 周口市中心血站检验科(周口 466000);
2 周口市第二人民医院妇产科(周口 466000)

收稿日期:2021-03-09 出版日期:2021-09-20 发布日期:2021-11-24

Confirmation of nonresponsiveness reactivity of HBV nucleic acid detection in blood donors and analysis of follow-up results

GAO Lei¹, ZHAO Mei², GAO Wenjie¹

1 Department of Laboratory Medicine,Zhoukou Central Blood Station, Zhoukou 466000, China;
2 Department of Obstetrics and Gynecology, Second People's Hospital of Zhoukou City, Zhoukou 466000, China

Received:2021-03-09 Online:2021-09-20 Published:2021-11-24

摘要/Abstract

摘要： 目的分析献血者乙型肝炎病毒(HBV)核酸检测非重复反应性确认及追踪结果。方法对1 200例于我站无偿献血者的血液样本进行非重复反应性确认,补充乙肝“两对半”检测,并对其部分单项核酸检测反应的患者予以回访结果追踪。结果 1 200份无偿献血血液样本单项核酸检测有反应性者150份(12.50%)。150份单项核酸检测有反应性样本经重新病毒核酸检测后,仍有58例样本有反应性(38.67%)、92例样本为非反应性(61.33%)。其中有18例献血者实际召回抽血,完成两次追踪,第一次追踪60~125天,第二次追踪间隔天数在160~356天,第一次追踪HBV DNA有反应(+)8例,第二次追踪仍存在反应(+);7例HBV DNA有反应者存在抗-HBc阳性(+)。核酸反应检测HBV-DNA阳性值10~17有37.25%,高于核酸反应检测值<10的4.17%(P<0.05)。结论部分单项核酸检测反应无偿献血者存在一定的输血传播HBV风险,多为HBV隐匿性感染,此时需要重视其输血情况,屏蔽单项核酸反应性献血者。

关键词: 核酸检测, 献血者, 乙型肝炎病毒, 非重复反应性确认, 追踪

Abstract: Objective To analyze the results of nonrepetitive reactivity of HBV nucleic acid detection in blood donors. Methods 1 200 blood samples from our station were confirmed by nonrepetitive reaction, supplemented with the “two to half” test of hepatitis B, and some patients with single nucleic acid detection were followed up. Results One hundred and fifty samples (12.50%) were reactive in single nucleic acid detection in 1 200 blood samples. After the virus nucleic acid detection, 58 samples were reactive (38.67%), 92 were nonreactive (61.33%). 18 donors were actually recalled and drew blood, completed two tracking. The first tracking time was 60-125 days, the second time interval was 160-356 days. In the first tracking 8 cases had HBV DNA reaction (+) , which still had reaction in the second tracking; 7 HBV DNA positive donors had HBC antibody (+).The proportion of HBV-DNA positive with 10-17 value in mucleic acid reaction test(37.25%) was higher than that with value below 10(4.17%,P＜0.05). Conclusion Some donors with single nucleic acid detection reaction have a certain risk of transmission of HBV, most of them had hidden infection of HBV. At this time, we should pay attention to the blood transfusion and avoid the single nucleic acid reactive donors.

Key words: nucleic acid detection, blood donors, hepatitis B virus, nonrepetitive reactivity confirmation, tracking

高磊, 赵梅, 高文杰. 献血者HBV核酸检测非重复反应性确认及追踪结果分析[J]. 广州医药, 2021, 52(5): 83-87.

GAO Lei, ZHAO Mei, GAO Wenjie. Confirmation of nonresponsiveness reactivity of HBV nucleic acid detection in blood donors and analysis of follow-up results[J]. Guangzhou Medical Journal, 2021, 52(5): 83-87.

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献血者HBV核酸检测非重复反应性确认及追踪结果分析

Confirmation of nonresponsiveness reactivity of HBV nucleic acid detection in blood donors and analysis of follow-up results

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