Fmr1基因敲除小鼠悬尾实验的观察

doi:10.3969/j.issn.1000-8535.2016.04.003

广州医药 ›› 2016, Vol. 47 ›› Issue (4): 9-11.DOI: 10.3969/j.issn.1000-8535.2016.04.003

Fmr1基因敲除小鼠悬尾实验的观察

胡丽婵¹, 郭艺², 孙祺章¹, 余国汉¹, 黄月玲³, 戴丽军³, 党亚梅¹, 黄雄¹, 陈盛强², 黄海樱²

1 广州医科大学附属脑科医院,广州市惠爱医院(广州 510370)
2 广州医科大学附属第二医院(广州 510260)
3 广州医科大学实验动物中心(广州 510182)

收稿日期:2016-04-01 出版日期:2016-07-20 发布日期:2021-12-02
通讯作者: 郭艺,E-mail: chenshengq66@gmail.com
基金资助:
广东省科技计划项目 (2013B021800075, 2015A0030302090);广州市科技计划项目(2014Y2-00046);广东省中医药局项目(B1533015);广东市卫生局科技项目(20141A10020,20151A011080)

Observation of tail suspension test in Fmr1 gene knockout mice

Hu Lichan, Guo yi, Sun Qizhang, Yu Guohan, Huang Yueling, Dai Lijun, Dang Yamei, Huang Xiong Chen Shengqiang, Huang Haiying,

Hu Lichan,Sun Qizhang,Yu Guohan, et al. The Brain Hospital affiliated to Guangzhou Medical University, Guangzhou 510370, China
Guo yi, Chen Shengqiang,Huang Haiying,et al. Institute of Neuroscience of the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China
Huang Yueling, Dai Lijun.The Laboratory Animal Research Center of Guangzhou Medical University,Guangzhou 510182,China

Received:2016-04-01 Online:2016-07-20 Published:2021-12-02

摘要/Abstract

摘要： 目的对不同周龄的KO小鼠与WT小鼠进行悬尾实验进行观察,探讨KO小鼠与WT小鼠的行为差别。方法采用健康的试验动物180只分两组:①KO组(4、6、8周龄,各周龄30只,雌雄各半,共90只)②WT组(4、6、8周龄,各周龄30只,雌雄各半,共90只);通过悬尾实验观察性别,年龄对不动时间的影响。结果同龄KO雌性小鼠比雄性小鼠的静止时间差别不大;随着年龄增大,静止时间增长。同龄同性别的KO鼠比WT鼠的不动时间长。P<0.05;同龄雄性小鼠比雌性小鼠的不动时间短; 随年龄增长各种系小鼠不动时间增长,KO鼠的不动时间比WT鼠长,P<0.05。结论 KO小鼠存在抑郁行为表型。

关键词: 悬尾实验, 脆性X综合征, 抑郁行为

Abstract: Objective To observe tail suspension test in Fmr1 gene knockout mice and to explore whether there are differences in mobility of KO and WT mice. Methods 180 test mice were divided into two groups: ① KO group (4,6,8 weeks old, each age group of mice is 30, male and female in half, a total of 90) ② WT group (4,6, 8 weeks old, each group of mice is 30, male and female on half, a total of 90). Through forced swimming test and tail suspension test to observe gender, age effect on immobility time. Results With the same age of the same sex,the KO mice's immobility time was longer than WT mice's. P<0.05. With the same age,the male mice's immobility time was shorter than female mice's. With the age increase, the immobility time of KO mice was longer than WT mice. P<0.05. Conclusion Fmr1 gene knockout mice have anxiety and depressive behavior.

Key words: Fragile X Syndrome, Tail suspension test, Depressive behavior

胡丽婵, 郭艺, 孙祺章, 余国汉, 黄月玲, 戴丽军, 党亚梅, 黄雄, 陈盛强, 黄海樱. Fmr1基因敲除小鼠悬尾实验的观察[J]. 广州医药, 2016, 47(4): 9-11.

Hu Lichan, Guo yi, Sun Qizhang, Yu Guohan, Huang Yueling, Dai Lijun, Dang Yamei, Huang Xiong Chen Shengqiang, Huang Haiying,. Observation of tail suspension test in Fmr1 gene knockout mice[J]. Guangzhou Medical Journal, 2016, 47(4): 9-11.

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Fmr1基因敲除小鼠悬尾实验的观察

Observation of tail suspension test in Fmr1 gene knockout mice

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