关键词: VITEK-MS质谱鉴定系统, 体液培养阳性, 直接鉴定

Abstract: Objective To find a fast method for detection of pathogens in positive culture bottles by using the VITEK-MS system. Methods VITEK-MS microbial identification system was used to directly identify the bacteria in the positive culture bottles, without culture on agar plates. The identification results were further compared with those by the traditional biochemical identification. Results Forty-seven bacterial strains were identified by traditional biochemical methods among 50 positive culture bottles, and 3 of them were negative. Of these 50 samples, thirty-one bacterial strains were identified by VITEK-MS and 3 were also negative. The sensitivity and specificity for direct VITEK-MS identification were 65.96% and 100%, and the clinical coincidence rate was 68%. The turn around time for identification was reduced from 24 to 2 hours. Conclusion Direct identification of bacterial pathogens in positive culture bottles by VITEK-MS could reduce turn around time, and lead to accurate and fast diagnosis.

Key words: VITEK-MS, Positive culture, Direct identification