肠道微生态-LPS-TLR4通路与新生儿坏死性小肠结肠炎炎症损伤的研究

doi:10.3969/j.issn.1000-8535.2022.03.011

广州医药 ›› 2022, Vol. 53 ›› Issue (3): 49-52.DOI: 10.3969/j.issn.1000-8535.2022.03.011

肠道微生态-LPS-TLR4通路与新生儿坏死性小肠结肠炎炎症损伤的研究

杨秀芳¹, 简伟华¹, 丁俊彩¹, 陈康², 施尚文¹

1 中山市人民医院新生儿科(中山 528403)
2 中山市人民医院检验中心(中山 528403)

收稿日期:2021-06-06 出版日期:2022-05-20 发布日期:2022-07-01
基金资助:
中山市卫健局立项(B20190035)

The study on intestinal flora-LPS-TLR4 pathway and inflammatory injury of neonatal necrotizing enterocolitis

YANG Xiufang ¹, JIAN Weihua ¹, DING Juncai ¹, CHEN Kang ², SHI Shangwen¹

1 Department of Neonatology, the People Hospital of Zhongshan City,Zhongshan 528403,China
2 Laboratory Center, the People Hospital of Zhongshan City,Zhongshan 528403,China

Received:2021-06-06 Online:2022-05-20 Published:2022-07-01

摘要/Abstract

摘要： 目的探讨新生儿坏死性小肠结肠炎(NEC)炎症损伤与肠道微生态-LPS-TLR4通路之间的关系。方法本研究收集2019年3月1日—2021年1月31日在中山市人民医院新生儿监护室确诊为NEC新生儿11例为实验组,随机选取30 例同期在新生儿科病房住院喂养顺利,排除NEC及败血症诊断的新生儿为对照组。采集2组新生儿的粪便标本,进行Real-time PCR表达谱分析2组粪便肠道菌群;取2组外周静脉血检测外周血单核细胞Toll样受体4(TLR4)和血清PCT、CRP、IL-6、SAA等指标,对比2组肠道菌群、外周血单核细胞TLR4和炎症指标水平,通过统计学分析组间差异。结果本研究结果提示实验组变形菌门占82%(9/11),厚壁菌门占9%(1/11),放线菌门占9%(1/11),对照组变形菌门占20%(6/30),厚壁菌门占73%(22/30),放线菌门占7%(2/30),2组患儿的粪便肠道菌群分布有差异(χ²=11.521,P<0.05);实验组患儿外周血单核细胞TLR4水平高于对照组,组间差异有统计学意义(P<0.001);实验组患儿血清PCT、CRP、IL-6和SAA等炎症指标高于对照组,组间差异有统计学意义(P<0.001)。结论 NEC患儿的肠道菌群以变形菌门为主,伴外周血单核细胞TLR4和外周血炎症指标升高。可见,肠道微生态-LPS-TLR4通路可能与新生儿坏死性小肠结肠炎炎症损伤相关,具体的机制仍需进一步深入研究。

关键词: 肠道菌群, Toll样受体4, 炎症指标, 新生儿坏死性小肠结肠炎

Abstract: Objective To investigate the relationship between intestinal flora-LPS-TLR4 pathway and the inflammatory injury of neonatal necrotizing enterocolitis (NEC). Methods Eleven neonates with NEC from March, 2019 to January, 2021 were enrolled as the experimental group, and 30 neonates without NEC and septicemia who were admitted in the department of neonatology in the same period were included as the control group. Faecal flora from the two groups were collected and analyzed by Real-time PCR. Toll-like receptor 4 (TLR4) and serum PCT, CRP, IL-6, SAA in peripheral blood were measured. The intestinal flora, the expression of TLR4 in peripheral blood leukocytes and inflammatory markers were compared between two groups. Results It showed that the ratio of Proteobacteria was 82% (9/11), Firmicutes was 9% (1/11), Actinobacteria was 9% (1/11) in the experimental group. In the control group, the ratio of Proteobacteria was 20% (6/30), Firmicutes was 73% (22/30), Actinobacteria was 7% (2/30). There was a significant difference in the distribution of faecal flora between the two groups (χ² = 11.521, P<0.05), and the level of TLR4 in peripheral blood of the experimental group was significantly higher than that of the control group (P＜0.001). The levels of serum PCT, CRP, IL-6 and SAA in the experimental group were significantly higher than those in the control group (P＜0.001). Conclusions The main intestinal flora of neonates with NEC is Proteobacteria, with elevated TLR4 expression and inflammatory markers in peripheral blood. Therefore, the intestinal flora-LPS-TLR4 pathway may be associated with inflammatory injury in neonatal necrotizing enterocolitis.The specific mechanism still needs further study.

Key words: intestinal flora, toll-like receptor 4, inflammatory markers, neonatal necrotizing enterocolitis

杨秀芳, 简伟华, 丁俊彩, 陈康, 施尚文. 肠道微生态-LPS-TLR4通路与新生儿坏死性小肠结肠炎炎症损伤的研究[J]. 广州医药, 2022, 53(3): 49-52.

YANG Xiufang. The study on intestinal flora-LPS-TLR4 pathway and inflammatory injury of neonatal necrotizing enterocolitis[J]. Guangzhou Medical Journal, 2022, 53(3): 49-52.

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肠道微生态-LPS-TLR4通路与新生儿坏死性小肠结肠炎炎症损伤的研究

The study on intestinal flora-LPS-TLR4 pathway and inflammatory injury of neonatal necrotizing enterocolitis

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