不明原因的自发流产与精子DNA损失的关系

doi:10.3969/j.issn.1000-8535.2019.02.014

广州医药 ›› 2019, Vol. 50 ›› Issue (2): 54-57.DOI: 10.3969/j.issn.1000-8535.2019.02.014

不明原因的自发流产与精子DNA损失的关系

刘成军¹, 涂艳琴¹, 王蔼明², 商微², 王伟周², 李敏², 沈玉良²

1 肇庆市西江医院生殖中心(肇庆526000)
2 海军总医院生殖中心(北京100048)

收稿日期:2019-01-10 出版日期:2019-03-20 发布日期:2021-12-17

Relationship between unexplained spontaneous abortion and sperm DNA damage

LIU Chengjun¹, TU Yanqin¹, WANG Aiming², SHANG Wei², WANG Weizhou², LI Min², SHEN Yuliang²

1 Reproductive Center, Xijiang Hospital, Zhaoqing 526000, China
2 Reproductive Center, Navy General Hospital, Beijing 100048, China

Received:2019-01-10 Online:2019-03-20 Published:2021-12-17

摘要/Abstract

摘要： 目的探讨一次不明原因流产与精子DNA损伤的关系。方法收集有一次不明原因流产史的患者作为实验组,同时以有正常妊娠史的患者为对照组,分别比较两组男方年龄、精子密度、精子活力、精液量和精子DNA断裂指数有无差异。以SPSS 16.0为统计软件,进行独立样本的t检验。结果两组的精液量、精子密度及活力均无差异,实验组男方年龄小于对照组(P<0.05),但实验组的DFI要高于对照组(P<0.01)。结论本研究对照组年龄高于实验组,而DFI正好相反。这说明不明原因的自发流产与男方精子DFI密切相关,随着DFI的增加,流产风险增加。

关键词: 不明原因自发流产, 精子DNA损伤, 一次, DFI

Abstract: Objective To investigate the relationship between unexplained abortion and sperm DNA damage. Methods Patients with a history of unexplained abortion were enrolled as an experimental group, and patients with a normal pregnancy history were used as a control group. The differences in age, sperm density, sperm motility, semen volume, and sperm DNA break index were compared between the two groups. The independent sample t test was performed using the statistical software of SPSS 16.0. Results There was no significant difference in semen volume, sperm density and motility between the two groups. The age of the male group in the experimental group was lower than that in the control group (P<0.05), but the DFI of the experimental group was higher than that of the control group, and the difference was statistical significance (P<0.01). Conclusion The age of the study control group was higher than that of the experimental group, while DNA fragmentation index DFI was the opposite, which indicated that unexplained spontaneous abortion was closely related to the male sperm DFI, and the risk of miscarriage may increase with the increase of DFI.

Key words: Unexplained spontaneous abortion, Sperm DNA damage, Once, DNA fragmentation index

刘成军, 涂艳琴, 王蔼明, 商微, 王伟周, 李敏, 沈玉良. 不明原因的自发流产与精子DNA损失的关系[J]. 广州医药, 2019, 50(2): 54-57.

LIU Chengjun, TU Yanqin, WANG Aiming, SHANG Wei, WANG Weizhou, LI Min, SHEN Yuliang. Relationship between unexplained spontaneous abortion and sperm DNA damage[J]. Guangzhou Medical Journal, 2019, 50(2): 54-57.

参考文献

[1] HAMAMAH S, FIGNON A, LANSAC J. The effect of male factors in repeated spontaneous abortion: lesson from in-vitro fertilization and intracytoplasmic sperm injection[J]. Hum Reprod Update, 1997,3(4):393-400.
[2] MICHAEL R, VIRRO M D,KJERSTEN L,et al. Sperm chromatin structure assay (SCSA)parameters are related to fertilization,blastocyst development, and ongoing pregnancy in vitro fertilization and intracytoplasmic sperm injection cycles[J]. Fertil Steril, 2004,81(5): 1289-1295.
[3] KHADEM N, POORHOSEYNI A, JALALI M, et al. Sperm DNA fragmentation in couples with unexplained recurrent spontaneous abortions[J]. Andrologia, 2014,46(2):126-130.
[4] 刘成军,王蔼明,商微. 精子DNA损失与不明原因复发性流产的关系[J]. 中华男科学杂志,2011,22(7):21-22.
[5] FERN ANDEZ J L, MURIEL L, GOYANES V, et al. Simple determination of human sperm DNA fragmentation with an improved sperm chromatin dispersion (SCD) test[J]. Fertil Steril, 2005, 84(4):833-842.
[6] CHO C, JUNG-HA H, WILLIS W D, et al. Protamine deficiency leads to sperm DNA damage and embryo death in mice[J]. Biol Reprod, 2003,69(1):211-217.
[7] OLIVA R. Protamines and male infertility[J]. Hum Reprod Update, 2006,12(4):417-435.
[8] SAKKAS D, SELI E, BIZZARO D, et al. Abnormal spermatozoa in the ejaculate: abortive apoptosis and faulty nuclear remodeling during spermatogenesis[J]. Reprod Biomed Online, 2003,7(4):428-432.
[9] SINHA HIKIM A P, SWERDLOFF R S. Hormonal and genetic control of germ cell apoptosis in the testis[J]. Rev Reprod, 1999, 4(1):38-47.
[10] AGARWAL A,SAID T M. Role of sperm chromatin abnormalitiesand DNA damage in male infertility[J]. Hum Reprod,2003,9 (4):331-345.
[11] KIM J M, GHOSH S R, WEIL A, et al. Caspase3 and caspase2 activated deoxyribonuclease are associated with testicular germ cell apoptosis resulting from reduced intratesticular testosterone[J]. Endocrinol, 2001, 142 (9): 3809-3816.
[12] MCVICAR C M,MCCLURE N,WILLIAMSON K, et al. Incidence of Fas positivity and deoxyribonucleic acid double2st randed breaks in human ejaculated sperm[J]. Fertil Steril, 2004, 81(1):767-774.
[13] MOUSTAFA M H, SHARMA R K, THORNTON J, et al. Relationship between ROS production, apoptosis and DNA denaturation in spermatozoa from patients examined for infertility[J ]. Hum Reprod, 2004, 19 (1): 129-138.
[14] AGARWAL A, SAID T M. Role of sperm chromatin abnormalities and DNA damage in male infertility[J]. Hum Reprod Update, 2003, 9(4):331-345.
[15] GANDINI L, LOMBARDO F, PAOLI D, et al. Study of apoptotic DNA fragmentation in human spermatozoa[J]. Hum Reprod, 2000, 15(4):830-839.
[16] BUNGUM M,HUMAIDAN P,SPANO M,et al. The predictive value of sperm chromatin t ructure assay (SCSA) parameters for the outcome of intrauterine insemination IVF and ICSI[J]. Hum Reprod, 2004, 19 (6):1401-1408.
[17] DURAN E H,MORSHEDI M, TAYLOR S,et al. Sperm DNA quality predict s intrauterine insemination, outcome: a prospective cohort study[J]. Hum Reprod, 2002, 17 (12):3122-3128.
[18] MORRIS I D, ILOTT S, DIXON L, et al. The spectrum of DNA damage in human sperm assessed by single cell gelelectrophoresis (Comet assay) and its relationship to fertilization and embryo development[J]. Hum Reprod, 2002, 17 (4): 990-998.
[19] BORINI A, TAROZZI N, BIZZARO D, et al. Sperm DNA fragmentation: paternal effect on early post-implantation embryo development in ART[J]. Hum Reprod, 2006, 21 (11): 2876-2881.
[20] EVENSON D P, JOST L K, MARSHALL D, et al. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic[J]. Hum Reprod, 1999, 14(4):1039-1049.
[21] LYNNE R, IOANNIS D G, SARAH J, et al. The effect of sperm DNA fragmentation on miscarriage rates: a systematic review and meta-analysis[J]. Hum Reprod, 2012, 27(10): 2908-2917.
[22] ATIF A K, KHALID A H, WAEL MOTAIR, et al. Secondary infertility and the aging male, overview[J]. Cent European, 2014, 67(2): 184-188.
[23] BANKS S, KING S A, IRVINE D S, et al. Impact of a mild scrotal heat stress on DNA integrity in murine spermatozoa[J]. Reprod, 2005,129(4):505-514.
[24] FOSSA S D, de ANGELIS P, KRAGGERUD S M, et al. Prediction of post-treatment spermatogenesis in patients with testicular cancer by flow cytometric sperm chromatin structure assay[J]. Cytometry, 1997,30(4):192-196.
[25] ERENPREISS J, HLEVICKA S, ZALKALNS J, et al. Effect of leukocytospermia on sperm DNA integrity: a negative effect in abnormal semen samples[J]. J Androl, 2002,23(5):717-723.
[26] GUADALUPE G, BENITO R, REBECA S, et al. Sperm DNA fragmentation in infertile men with genitourinary infection by Chlamydia trachomatis and Mycoplasma[J]. Fertil Steril, 2008, 90(2):328-334.

不明原因的自发流产与精子DNA损失的关系

Relationship between unexplained spontaneous abortion and sperm DNA damage

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价

[1]	邱明燕, 吴礼武, 刘伯念, 刘丽嫚. 一次性带冲洗球囊胃管的临床应用[J]. 广州医药, 2017, 48(5): 36-38.
[2]	黄韬, 吴世皓, 曾任创, 石宇强, 张信基. 一次性包皮切割缝合器与两种传统包皮环切术的应用比较[J]. 广州医药, 2017, 48(4): 73-76.
[3]	黄润镟. 一次性囊式宫颈扩张器用于治疗产后出血的临床研究[J]. 广州医药, 2017, 48(2): 102-104.